Composite fluorescent nano particle used for immunoassay and its preparation method
An immunological detection and nanoparticle technology, which is applied in the direction of measuring devices, scientific instruments, biological testing, etc., can solve the problems of difficult multi-index simultaneous detection, low resolution, photobleaching or photodegradation, etc., and achieve excellent chemical Effects of Stability and Biocompatibility
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Embodiment 1
[0015] Get 5 mg of CdS nanoparticles with a particle diameter of 1.9 nm, dissolve them in 120 ml of methanol containing 0.17% triethoxymercaptopropylsilane and 25% dimethyl sulfoxide, and use (CH 3 ) 4 NOH 5H 2 O to adjust the pH value between 10-11, after stirring the reaction for 12 hours, add 40ml10% trimethylchlorosilane solution, use (CH 3 ) 4 NOH 5H 2 O adjust the pH value between 10-11, stir the reaction for 12 hours, then concentrate in vacuo at 60°C to an oily liquid, add acetone:isopropanol=1:1 mixed solvent to precipitate CdS. After the precipitate was washed twice with acetone, 50ml of ethanol was added for ultrasonic redispersion, 2ml of concentrated ammonia water with a concentration of 23%, 10L of ethyl orthosilicate, and 2L of triethoxyaminopropylsilane were added, and the reaction was stirred for 6 hours to obtain a coating SiO 2 The luminescent particle sol with a layer thickness of 10nm was centrifuged at a speed of 5000rpm for 60 minutes, and the super...
Embodiment 2
[0017] Take 6mg of CdS nanoparticles with a particle size of 2.4nm as the luminescent core, carry out surface modification according to the steps in Example 1 to obtain a sol ultrasonically dispersed in ethanol, add 2ml concentration of 23% concentrated ammonia water, 20L ethyl orthosilicate, 4L triethoxyaminopropylsilane, stirred and reacted for 8 hours to obtain coated SiO 2 The luminescent particle sol with a layer thickness of 20 nm was subjected to gold deposition according to the reaction conditions in Example 1. Composite luminescent particles with a luminescence peak of 680 nm were finally obtained.
Embodiment 3
[0019] Take 10 mg of CdSe nanoparticles with a particle size of 3.6 nm as the luminescent core, and perform surface modification according to the steps in Example 1 to obtain a sol ultrasonically dispersed in ethanol, then add 2 ml of concentrated ammonia water with a concentration of 23%, 25 L of ethyl orthosilicate , 4L triethoxyaminopropylsilane, stirred and reacted for 8 hours to obtain coated SiO 2 The luminescent particle sol with a layer thickness of 25nm was transferred to the aqueous phase according to the method in Example 1, and 3ml containing 2nm gold particles (1×10 -3 mol / L) sol, stirred and reacted for 2 hours to obtain a composite luminescent particle whose core is CdSe with gold deposited on the surface with a thickness of 7nm. The luminescent peak of the composite particle is at 680nm.
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