Biodegradable mixed polymeric micelles for gene delivery

A polymer and polyester technology, applied in gene therapy, use of microcapsules, applications, etc., can solve the problems of replicating host immune responses, cytotoxic complaints, and only orientation.
CN1281355AInactive Publication Date: 2001-01-24三阳有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
三阳有限公司
Publication Date
2001-01-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

A biodegradable, mixed polymeric micelle used to deliver a selected nucleic acid into a targeted host cell contains an amphiphilic polyester-polycation copolymer and an amphiphilic polyester-sugar copolymer. The polyester-polycation copolymer forms an electrostatic interaction with polyanionic nucleic acids, and the polyester-sugar copolymer directs the micelle-nucleic acid complex to cells in vivo. Additional copolymers with similar properties may also be included. The composition improves delivery efficiency by providing a particulate gene carrier for which particle size and charge density are easily controlled by multivariate means. Various kinds of ligands and other functional compounds may be also be introduced using the composition. The composition may be used in a method for transforming a targeted host cell with a selected nucleic acid.
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Description

[0001] INTERNATIONAL REFERENCE TO RELATED APPLICATIONS This application claims the benefit of US Provisional Patent Application No. 60 / 069,551 (filed December 12, 1997). Background of the invention

[0002] The present invention relates to systems for gene delivery into eukaryotic cells. In particular, the present invention relates to compositions and methods for delivering selected nucleic acids into host cells via biodegradable mixed polymeric micelles containing amphiphilic polyester-polycationic copolymers and amphiphilic polyester-sugars copolymer.

[0003] As early as the mid-1950s, methods for the delivery of nucleic acids into tissue culture cells were established, see H.E. Alexander et al., Virology, Vol. 5, pp. 172-173 (1958). Since then, steady progress has been made in improving the in vivo and in vitro delivery of functional DNA, RNA and antisense oligonucleotides (inhibitors of RNA function). In the late 1970s, due to the integration of transfection technology ...

Claims

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