Transfection particles

Abstract

Transfection particles used to deliver nucleic acids into cells of higher eukaryotes in vivo and in vitro, including one or more nucleic acid molecules condensed by organic cationic molecules. The dispersed stable particles are formed by complexing the nucleic acid molecules with the same or different organic cation precursor molecules, rather than cross-linking between the nucleic acid molecules, and the precursor molecules are covalently bonded to each other on the nucleic acid template and made. For unambiguous cell orientation, the particles are loaded with orientation molecules, such as sugars. Preferred cationic precursor molecules are lipophilic detergents bonded to form lipids. The particle preferably contains only one nucleic acid molecule making it effective for gene therapy and delivery of large DNA molecules.

Description

technical field

[0001] The present invention relates to the field of delivery of nucleic acids into cells of higher eukaryotes, particularly for gene therapy. The present invention provides novel transfection particles for in vitro and in vivo transfection of higher eukaryotic cells. Background technique

[0002] A critical step in modern recombinant nucleic acid technology is the efficient transfection of cells with foreign genetic material both in vitro and in vivo.

[0003] Typically, recombinant viruses are used to efficiently deliver foreign genetic material into cells in vitro and in vivo (Mulligan, 1993). Recombinant viruses are often specific cells with advantages or disadvantages, depending on the individual experiment. In addition to foreign material, they also carry their own genetic material and are very susceptible to mutations during the process of replicating. Another disadvantage of in vivo viruses in organisms with immune systems is that viral particles s...

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