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Multiple strain microorganism ferment production method

A technology of microbial starter and production method, which is applied to bacteria, animal feed, animal feed, etc., can solve the problems of high elimination rate of dairy cows' tri-fat, excess energy in formula, and short service life, so as to maintain stable intestinal ecology and increase Yield and quality, efficacy in preventing and treating diarrhea

Inactive Publication Date: 2007-07-04
包头市天赋食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the research and development of concentrated feed for dairy cows is limited to the mixing of corn, bran, and cakes, the ratio of fish meal, meat and bone meal, feather meal, and corn protein, or the formulation research of adding enzymes, mineral elements, and antibiotics, resulting in Excess energy in the formula, improper combination of single amino acid in protein feed, severe lack of minerals, trace elements and vitamins, resulting in low feed conversion efficiency, low milk production, low milk fat rate and milk protein rate, ruminal acidosis of dairy cows, and nutritional and metabolic diseases High incidence of disease, high three-fat elimination rate and short service life of dairy cows affect the production potential of dairy cows
and high feed cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0043] Four, the specific implementation mode: (best embodiment of the present invention)

[0044] The production steps of starter are as follows:

[0045] The original slant strain → pure strain separation → access to the slant → shake tube culture → enlarged culture in the triangular flask → tank culture → mix the fermentation products of fourteen strains in different proportions → starter.

[0046] The production steps of starter are as follows:

[0047] The production steps of multi-strain microbial starter are as follows:

[0048] Bacteria culture: (1) Original slant strains: 1) 8 kinds of bacteria: Cellulomonas bizotea; Cellulomonas flavigena; Bacillus subtilis 10264 (Bacillus subtilis); Bacillus coagulans Bacillus coagulans; Lactobacillus acidophilus; Lactobacillus amylopHllus; propionibacterium freudenreichii, Bacillus subtilis TQ13055 (Bacillus subtilis);

[0049] 2) 3 kinds of yeast: Candida utilis; Saccharomyces diastaticus; Saccharomyces cerevisiae;

[0050] 3)...

Embodiment 1

[0076] Embodiment one: produce the preparation method of 1000kg multi-strain microbial starter and its application in concentrated feed for dairy cows: with crop corn 410Kg, sunflower cake 160Kg, gourd hemp cake 135Kg, bean cake 130Kg, bran 70Kg, stalk powder 50Kg , minerals 40Kg (including: CaCO 3 40-60%, CaHPO 4 .2H 2 O15-25%, NaCl20-30%, MgSO 4 .7H 2 O0.3-0.8%, Se20-70PPm, I1000-150PPm, Co20-30PPm, Mn0.05-0.15%), urea 5Kg and other raw materials are pulverized and sieved through 20 meshes, and the amount of water added is 100Kg. Hours, add 100L of mixed starter to 1000Kg of moistened raw materials. The mixing ratio of raw materials and water is 1:0.5-1. After mixing evenly, pack them into bags for fermentation. Each bag is 20-40kg. Fermentation time: according to the strain It depends, such as using natural fermentation for 4-7 days in summer, 12-17 days in spring and autumn, and 1-2 months in winter. After fermentation, it will be a finished product.

[0077] Finishe...

Embodiment 2

[0080] Embodiment two: produce the preparation method of 1000kg multi-strain microbial fermentation agent and its application in concentrated feed for dairy cows: with crop corn 500Kg, sunflower cake 170Kg, gourd hemp cake 100Kg, bean cake 100Kg, bran 50Kg, stalk powder 40Kg , minerals 32Kg (including: CaCO 3 40-60%, CaHPO 4 .2H 2 O15-25%, NaCl20-30%, MgSO 4 .7H 2 O0.3-0.8%, Se 20-70PPm, I 100-150PPm, Co20-30PPm, Mn0.05-0.15%), urea 8Kg and other raw materials are pulverized, sieved through 20 mesh, and the amount of water added is 100Kg after adding water. Feed for 1 hour, add 100L of mixed starter to 1000Kg of moistened raw materials, the mixing ratio of raw materials and water is 1:0.5-1, mix well and then pack into bags for fermentation, each bag is 20-40kg, fermentation time: according to Depending on the strain, such as natural fermentation for 4-7 days in summer, 12-17 days in spring and autumn, and 1-2 months in winter, the finished product will be fermented.

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Abstract

The invention relates to a preparing method for poly strains microbe leaven and its application in cow concentrated feed. It belongs to microbe ferment field of bioengineering. The invention adopts multiple microbe strains, and combines them in ratio to produce the leaven. Evenly mixing the raw material and straws, then adding to activated leaven, packing them to the container to ferment for obtaining the finished product. The feeding stuff manufactured by the leaven raises the milk output 0.5 to 1kg per day, prolongs the stage of lactation, inhibits the intestinal canal bacteria growth, increases the cow appetite, raises the feeding stuff utilization factor by 15%. The analysis to the feeding stuff shows that crude protein is increased by 20-25%, the raw fiber is decreased by 10-15%.

Description

1. Technical field: [0001] The invention relates to a preparation method of a multi-strain microbial fermentation agent, which belongs to the field of microbial fermentation in biological engineering. 2. Background technology: [0002] At present, the research and development of concentrated feed for dairy cows is limited to the mixing of corn, bran, and cakes, the ratio of fish meal, meat and bone meal, feather meal, and corn protein, or the formulation research of adding enzymes, mineral elements, and antibiotics, resulting in Excess energy in the formula, improper combination of single amino acid in protein feed, severe lack of minerals, trace elements and vitamins, resulting in low feed conversion efficiency, low milk production, low milk fat rate and milk protein rate, ruminal acidosis of dairy cows, and nutritional and metabolic diseases High incidence of disease, high three-fat elimination rate and short service life of dairy cows affect the production potential of da...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/18A23K10/12A23K10/30A23K10/37A23K20/20A23K50/15
CPCY02P60/87
Inventor 方向东丁友昉秦刚
Owner 包头市天赋食品有限公司
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