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Prepn of osteological material containing nano phase calcium phosphate, collagen and alginate

A technology of alginate and calcium phosphate salt, applied in the field of bioengineering, can solve the problems of limited immune rejection, infectious disease risk, etc., and achieve excellent biocompatibility, good biocompatibility, reasonable strength and porosity. Effect

Inactive Publication Date: 2002-02-27
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, autologous bone and allogeneic bone have the problems of limited quantity and risk of immune rejection and infection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The materials used are type I collagen gel (concentration: 1% solid content, the collagen contained is purified cowhide collagen) purchased from Yierkang Company, analytically pure CaCl 2 ·6H 2 O. Analytical pure Na 2 HPO 4 , Sodium Alginate from Acros Co.

[0018] (1) Dissolve 20g collagen gel in 300ml 0.5M acetic acid solution, slowly add 18.3ml 1mol / l CaCl dropwise 2 and 11ml 1mol / l Na 2 HPO 4 , stirring with a magnetic stirrer while adding dropwise;

[0019] (2) continue to stir, slowly dropwise simultaneously the NaOH solution of 0.5mol / l to pH value 7;

[0020] (3) Let the solution stand for 1 day, remove the supernatant, centrifuge and separate the precipitate, wash it repeatedly with deionized water three times, put it into a lyophilizer for lyophilization, and grind it to obtain a dry powder for subsequent use;

[0021] (4) Mix 2.4 grams of the dry powder prepared in step (3) with 0.6 grams of sodium alginate, and then add 5 milliliters of distilled wate...

Embodiment 2

[0026] The materials used are type I collagen gel from Tianjin Second Affiliated Hospital (concentration: solid content 1%, the collagen contained is purified bovine Achilles tendon collagen), analytically pure CaCl 2 2H 2 O, analytically pure (NH 4 ) 2 HPO 4 , Sodium alginate came from Beijing Xisi Chemical Raw Material Company.

[0027] (1) 20g collagen gel dissolved in 300ml 0.1M HNO 3 Into the solution, slowly drop 10ml 1mol / l CaCl 2 and 10ml 1mol / l (NH 4 ) 2 HPO 4 , stirring with a magnetic stirrer while adding dropwise;

[0028] (2) continue to stir, slowly dropwise simultaneously the NaOH solution of 0.5mol / l to pH value 7;

[0029] (3) Let the solution stand for 4 days, remove the supernatant, centrifuge and separate the precipitate, wash it repeatedly with deionized water three times, put it into a lyophilizer for lyophilization, and grind it to obtain a dry powder for subsequent use;

[0030] (4) Mix 1.8 grams of the dry powder prepared in step (3) with 0.6...

Embodiment 3

[0035] The materials used are liquid type I collagen purchased by CELLON company (concentration: 0.3% solution pH value is adjusted by HCl and the contained collagen is purified bovine skin collagen), analytically pure CaCl 2 ·6H 2 O, analytically pure H 3 PO 4 (content>=85% density 1.689 g / ml), sodium alginate from Acros Co.

[0036] (1) Add dropwise 1.125ml H2O dissolved in 10ml deionized water to 100ml collagen solution 3 PO 4 , stirring with a magnetic stirrer while adding dropwise;

[0037] (2) Weigh 6.01g of CaCl with good crystallization 2 6H 2Dissolve O in 20ml of deionized water to make it completely dissolved, add it dropwise to the solution prepared in the previous step, and continue to stir for 1 hour;

[0038] (3) continue to stir, slowly dropwise simultaneously the NaOH solution of 0.75mol / l to pH value 7;

[0039] (4) Let the solution stand for 1 day, remove the supernatant, centrifuge and separate the precipitate, wash it repeatedly with deionized water...

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Abstract

The preparation method of bone material containing nanometer phase calcium phosphorus salt, collagen and aliginate includes the following steps: slowly drop-adding solution containing calcium iron into acid-soluble collagen solution, then slowly drop-adding aqueous solution containing phosphoric acid radical ion into said solution while stirring, and slowly drop-adding alkali liquor to regulate pH value while stirring, standing still and removing supernatant fluid, centrifugal separation, precipitation, grinding to obtain dried powder, mixing the dried powder with sodium alginate and water toobtain a product, placing said product into mould, forming, demoulding, soaking in distilled water, drying to obtain the invented bone material with excellent biological compatibility and imitation bone property in structure, reasonable strengthen and void volume. Said invented bone material can be used as implanted degradable bone material.

Description

Technical field: [0001] The invention relates to a preparation method of a bone material containing nano-phase calcium phosphate salt, collagen and alginate, belonging to the technical field of bioengineering. Background technique: [0002] The research and development of bone repair has always been the direction of efforts of scientists in the field of biomedical engineering. A variety of bone repair materials including metals, ceramics, polymers, and their composite materials have been developed, but due to the lack of mechanical properties, biocompatibility, and degradability, the actual bone repair materials used in surgery are not effective. The best ones are autograft and allograft. However, autologous bone and allogeneic bone have the problems of limited quantity and the risk of immune rejection and infection. Invention content: [0003] The purpose of the present invention is to propose a preparation method of bone material containing nano-phase calcium phosphate...

Claims

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Application Information

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IPC IPC(8): A61F2/28A61K38/39A61L27/24A61L27/44A61L27/56
Inventor 崔福斋张曙明廖素三冯庆玲
Owner TSINGHUA UNIV
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