Method of in vitro culturing medicine ox gallstone with natural ox gall

A technology for ox gallstones and ox bile is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, unknown raw materials, etc., which can solve the problems that ox gallstones cannot meet medicinal requirements, and achieve good social and economic benefits. The effect of low production cost and high stone formation efficiency

Inactive Publication Date: 2002-05-08
武汉键民大鹏药业有限公司
View PDF2 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the bovine gallstones made by the above method cannot meet the medic

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] 1. Implementation mode one:

[0022] (1) Obtaining natural bovine bile: Take out fresh bovine bile, adjust the taurine content to 7% according to the requirements of high-quality bezoar, put it in a sterilization tank, and store it at low temperature for later use.

[0023] (2) Preparation of fermented bovine bile: Put bovine bile in a fermentation tank, and inoculate the combined bacterial solution of Escherichia coli and Proteus into bovine bile at the ratio of 100 ml of bacterial solution per 10000 ml of bovine bile, and ferment for 80 hours for standby use .

[0024] (3) Preparation of calcium bilirubin compound:

[0025] ① Take 20000ml of fermented beef bile and add 22000ml of clarified saturated calcium oxide solution, stir, boil, and after cooling, take out the brown-red precipitate.

[0026] ②Add 10000ml of pure water to the brown-red precipitate, stir well, and then pour off the supernatant, add bilirubin until its content reaches more than 45%, then add 3g of zinc...

Example Embodiment

[0031] 2. Implementation mode two:

[0032] (1) Obtaining natural cattle bile: When killing cattle, under aseptic operation conditions, take out the gallbladder, select the one that is free of impurities, uncontaminated and golden yellow, and pour the bile into the sterilization tank. According to the requirements of high-quality bezoar, Adjust the taurine to 6% and store it at low temperature for later use.

[0033] (2) Preparation of fermented bovine bile: put bovine bile in a fermenter, and inoculate E. coli into bovine bile at a ratio of 200 ml of bacterial solution per 10,000 ml of bovine bile, and ferment for 96 hours before use.

[0034] (3) Preparation of calcium bilirubin compound:

[0035] ① Take 20,000 ml of fermented beef bile and add 60,000 ml of clarified saturated calcium hydroxide solution, stir, boil, and cool to 50°C, and take out the brown-red precipitate.

[0036] ②Add 10000ml of cold boiled water to the brown-red precipitate, stir and let stand for 30 minutes,...

Example Embodiment

[0041] 3. Implementation mode three:

[0042] (1) Obtaining natural beef bile: Take out fresh beef bile, put it in a sterilization tank, and store it at low temperature for later use.

[0043] (2) Preparation of fermented bovine bile: Put bovine bile in a fermentation tank, and inoculate enterococcus into bovine bile at the ratio of 10000ml bovine bile to inoculate 300ml of bacterial solution, and ferment for 92 hours before use.

[0044] (3) Preparation of calcium bilirubin compound:

[0045] ① Take 29600ml of fermented beef bile, filter and add 88800ml of clarified saturated calcium oxide solution, stir, boil, and take out the brown-red precipitate after cooling.

[0046] ②The brown-red precipitate is fully stirred with water. After standing, the supernatant is poured and the bilirubin is added to the content of 45% or more, and then 100g of cholic acid, 40g of deoxycholic acid, 3g of zinc sulfate and 3g of magnesium sulfate are added. Stir and mix well.

[0047] (4) Cultivate me...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

Natural ox gall is first taurine regulated and bacteria fermented to prepare fermented ox gall. The fermented ox gall is gall is then mixed with clear saturated calcium salt solution through stirring, heated to boil, filtered to obtain red brown precipitate, which is stirred, washed, filtered to eliminate supernatant and mixed with bilirubin, cholalic acid, deoxycholic acid, inorganic salt and other effective components to obtain composite bilirubin calcium. Fermented ox gall and bilirubin calcium in certain proportion are mixed and the pH of mixture is regulated to below 6.8 and directional off axis rotated to form gallstone, and the gallstone is rest cultured, taken out and dried. The said process can obtain ox gallstone product with the same characteristics, structure, component and medicinal effect as high quality natural bezoar.

Description

1. Technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a method for manufacturing a traditional Chinese medicine raw material. 2. Background technology [0002] Bovine gallstone, namely bezoar, is a precious Chinese herbal medicine. It has the functions of clearing heart, clearing phlegm, resuscitating, cooling liver, relieving wind, clearing heat and detoxifying. Many famous and precious Chinese patent medicines in our country, such as Angong Niuhuang Pills and Liushen Pills, are all formulated with bezoar as the main medicine. However, the natural stone formation rate in cattle is only 1-2%. Therefore, the source of medicine is extremely scarce, expensive, and dependent on imports, which not only costs a lot of foreign exchange, but also restricts the development of my country's traditional medicine industry. [0003] Since 1956, Chinese medical workers have been actively engaged in the research of artificial bezo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/413
Inventor 蔡红娇
Owner 武汉键民大鹏药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap