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Process for preparing internally degradable tubular liver tissue frame material

A technology of frame material and liver tissue, which is applied in the field of three-dimensional liver tissue frame materials, can solve the problems of material exchange, nutrients cannot be replenished, and metabolic waste cannot be discharged in time, so as to achieve the effect of prolonging the survival period

Inactive Publication Date: 2002-08-14
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The literature "B.L.Seal, T.C.Otero, A.Panitch, MaterialsScience and Engineering: R: Reports 34 (2001) 147" pointed out that the possible reason is that the unfavorable material exchange in the system makes the nutrients not replenished and the metabolic wastes cannot be discharged in time. Walk

Method used

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  • Process for preparing internally degradable tubular liver tissue frame material
  • Process for preparing internally degradable tubular liver tissue frame material
  • Process for preparing internally degradable tubular liver tissue frame material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Heat and mix sliced ​​paraffin wax and stearic acid in a 1:1 (W / W) ratio in a water bath at 60°C to make a blank, and then carve it into a fine mesh mold.

[0037] (2) Dissolve a certain amount of polylactic acid (Mw: 3×10) in 1,4-dioxane to make a solution with a concentration of 5 (W / V)% and add 0.5 (W / V)% The pore-forming agent ammonium bicarbonate is uniformly mixed and coated on the mold in (1), freeze-dried and formed into a film.

[0038] (3) Soak the sample with 0.05M hydrochloric acid to remove the pore-forming agent, and demold it with xylene to prepare a multi-tubular scaffold material with a pore diameter of 50-100 μm and a porosity of 45%.

[0039] (4) After the above sample is activated with 0.5M NaOH solution, the excess sodium hydroxide is neutralized with 0.1mol / L hydrochloric acid solution, and rinsed with deionized water; in 0.5wt% 1-ethyl-3- Soak in (3-dimethylaminopropyl)carbodiimide hydrochloride aqueous solution at 4°C for 12h, use 2-(N-morphol...

Embodiment 2

[0044] (1) Paraffin wax and stearic acid were mixed in a beaker at a ratio of 1.25:1 (W / W) to 65°C to make a blank, and then carved into a fine mesh mold.

[0045] (2) Take a certain amount of polylactic acid and polyglycolic acid copolymer (70:30M / M) and dissolve it in 1,4-dioxane to make a solution with a concentration of 15 (W / V)% and add 0.5 (W / V)% disodium hydrogen phosphate, uniformly mixed and coated on the mold in (1), freeze-dried and film-formed.

[0046] (3) Soak the sample in deionized water to remove the pore-forming agent, and demold it with xylene to prepare a multi-tubular scaffold material with a pore diameter of 80-150 μm and a porosity of 60%.

[0047] (4) After the above sample is activated with 0.75M NaOH solution, the excess sodium hydroxide is neutralized with 0.5mol / L hydrochloric acid solution, and rinsed with deionized water, in 0.75wt% 1-ethyl-3- Soak in (3-dimethylaminopropyl) carbodiimide hydrochloride aqueous solution at 4°C for 24 hours, use 2-(...

Embodiment 3

[0051] (1) Paraffin wax and stearic acid are mixed in a beaker at a ratio of 1.4:1 (W / W) to 70°C to make a blank, and then carved into a fine mesh mold.

[0052] (2) Take a certain amount of polycaprolactone (Mw: 5×10 4 ) was dissolved in 1,4-dioxane to make a solution with a concentration of 25 (W / V)%, and 1 (W / V)% disodium hydrogen phosphate was added, mixed evenly and coated on (1 ) on the mold, freeze-dried and film-formed.

[0053] (3) Soak the sample in deionized water, and demold it with xylene to obtain a multi-tubular scaffold material with a pore diameter of 100-300 μm and a porosity of 75%.

[0054] (4) After the above-mentioned sample is activated with 1M NaOH solution, excessive sodium hydroxide is neutralized with 1mol / L hydrochloric acid solution, and rinsed with deionized water; In dimethylaminopropyl) carbodiimide hydrochloride aqueous solution, soak 36h at 4 ℃, adopt 2-(N-morpholine)-ethane-sulfonic acid as stabilizer, adjust pH=6.0, in 1wt% Chitosan aceti...

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Abstract

An internally degradable 3D frame material with tubular structure for culturing liver cells in body is prepared through such steps as preparing mould from paraffine and stearic acid, preparing degradable high-molecular solution of polylactic acid, adding pore-forming agent, coating on the mould, physical and chemical treating, treating it with anti-coagulating medicine, and sterilizing. Its advantages include high cell and blood compatibility, and high survival period of liver cells.

Description

technical field [0001] The invention relates to a three-dimensional liver tissue frame material with a tubular structure for culturing hepatocytes in vivo, belonging to the technical field of bioengineering. Background technique [0002] Liver transplantation is an effective treatment for acute and chronic liver failure caused by extensive damage / loss of liver tissue and end-stage liver disease. However, the severe shortage of donor livers has become the main obstacle to the development of this treatment. To solve this contradiction, it is necessary to solve the risk of infection transmission between humans and animals and the greater immune rejection of xenogeneic liver transplantation. At present, extracorporeal artificial liver assisting devices, including non-biological type, biological type and hybrid type, can assist and support liver function in a short time, serve as a bridge for patients waiting for liver transplantation, or support liver function to survive the dan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L29/04A61L29/14A61L31/04
Inventor 冯庆玲崔福斋李大鹏王小红徐迎新
Owner TSINGHUA UNIV
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