Process for preparing tetra-hexose ganglioside monosialate

A ganglioside and monosialic acid technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of reduced curative effect, time-consuming, expensive and other problems, and achieve improved safety and environmental protection properties, reducing the amount of organic solvents used, improving yield and purity

Inactive Publication Date: 2002-11-13
LITUO DEV GUANGZHOU CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the similar structure and properties of ganglioside components, the traditional method of separating and purifying a single component is time-consuming, laborious, and requires the use of a large amount of harmful organic solvents. At present, similar imported products for domestic clinical applications are expensive.
The length of the hydrophilic and hydrophobic ends of gangliosi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment ( 1

[0014] 1. Prepare acetone powder with fresh pig brains: collect fresh pig brains from the meat joint factory, remove bones, meninges, skull base blood vessels, etc., wash with distilled water, add 10 times of ice acetone after weighing, and homogenize at 6000 rpm After 30 minutes, pour it into a 100-mesh stainless steel sieve to collect the residue, filter the filtrate through a 400-mesh stainless steel sieve to collect the residue, and continue to filter the filtrate through a 800-mesh stainless steel sieve to collect the residue. All the residues were collected and rotary evaporated under reduced pressure in a 40°C water bath to constant weight to obtain porcine brain acetone powder with a yield of about 1 / 8-1 / 10. Recycled acetone can be reused after distillation.

[0015] 2. Total lipid extraction and fractionation:

[0016] The above-mentioned acetone powder was weighed, firstly, 10 times volume of chloroform:methanol:water (50:50:10, v / v / v, the same below) was added, sti...

Embodiment ( 2

[0048] 1. Preparation of acetone powder:

[0049] Collect fresh pig brains from the meat joint factory, remove bones, meninges, skull base blood vessels, etc., wash with distilled water, add 6-20 times of ice acetone after weighing, homogenize at 6000 rpm for 30 minutes, pour into a 100-mesh stainless steel sieve , collect the residue, filter the filtrate through a 400-mesh stainless steel sieve, collect the residue, continue to filter the filtrate through an 800-mesh stainless steel sieve, and collect the residue. All the residues were collected and rotary evaporated under reduced pressure in a 40°C water bath to constant weight to obtain pig brain acetone powder with a yield of about 1 / 8-10. Recycled acetone can be reused after distillation.

[0050] 2. Total lipid extraction and fractionation:

[0051] The above-mentioned acetone powder was weighed, firstly, 5-20 times volume of chloroform:methanol:water (50:50:10, v / v / v, the same below) was added, stirred for 60 minutes,...

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Abstract

A process for preparing monosialic acid tetra-hexose ganglioside preparation from pig's brain includes such steps as ultrafilter membrane separation, chromatography by Iatrobeads column and DEAE sephadex A-25 column, and efficient liquid-phase chromatography technique. In the preparation process, it retains the GMI amphipathy physico-chemical characteristic of ganglioside. Its advantages are highpurity (more than 95%), low cost, environment protection and high curative effect.

Description

technical field [0001] The invention belongs to the field of biochemical pharmacy, and in particular relates to a method for preparing a monosialotetrahexosyl ganglioside preparation. Background technique [0002] Gangliosides are a class of amphiphilic molecules composed of sphingosine, fatty acids, and oligosaccharides containing sialic acid residues. They are widely found on the surface of mammalian cell membranes, especially in the central nervous system. They are the main components of nerve cell membranes. One of the lipid components. Gangliosides play an indispensable role in the occurrence, differentiation and growth of the nervous system, and are also very important for the repair of the nervous system after injury. They can promote nerve growth and regeneration, promote synapse formation, restore innervation function, protect Nerve cell membrane, etc. [0003] Monosialotetrahexosyl ganglioside (ganglioside GM1) is one of the main gangliosides, which can promote n...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H15/10
Inventor 钟春玖胡必文周康刘康达
Owner LITUO DEV GUANGZHOU CITY
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