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Blood substitute of hemoglobin microcapsule and preparing method thereof

A technology of blood substitutes and hemoglobin, which is applied in the field of hemoglobin microcapsule blood substitutes and its preparation, can solve the problems of low concentration of embedded hemoglobin, easy peroxidation of phospholipids, and poor stability, so as to improve the embedding rate and facilitate amplification , the effect of reducing particle size

Inactive Publication Date: 2003-05-28
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hemoglobin liposomes still have the following disadvantages: poor stability during storage and after infusion; phospholipids have potential effects on the reticuloendothelial system; phospholipids are prone to peroxidation; the concentration of embedded hemoglobin is low
However, the particle size he prepared is a bit too large, 200 μm, while the diameter of the thinnest capillary in the human body is only 4 μm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Dissolve 50mg of 30,000 (5000) (PLA / MPEG molecular weight is 30,000, of which MPEG molecular weight is 5000, the same below) in 3ml of dichloromethane, add 600ul bovine hemoglobin, 10000rpm colostrum for 30s, pour 100ml of NaCl 0.9%, PVA6 % double-emulsion, double-emulsified at 8000rpm for 1min, then poured into 200ml of physiological saline, and magnetically stirred at 1000rpm under normal temperature and pressure for 1.5 hours. Finally, centrifuge and wash four times with 10,000 g of physiological saline, and collect hemoglobin microcapsule precipitates. (The weight ratio of the copolymer and the hemoglobin in the microcapsule is 1:2, and the particle size is less than 4 μm).

Embodiment 2

[0044] Dissolve 40 mg of 60,000 (5000) in a mixed solvent of 2 ml of dichloromethane and 1 ml of ethyl acetate, add 800 ul of hemoglobin, perform primary emulsification at 10,000 rpm for 30 seconds, pour into 100 ml of 1% glycerin, PVA6% double emulsion, and double emulsify at 8,000 rpm for 1 min. Then pour it into 200ml of physiological saline, and stir it by magnetic force at 1000rpm under normal temperature and pressure for 1.5 hours. Finally, centrifuge and wash four times with 10,000 g of physiological saline, and collect hemoglobin microcapsule precipitates. (The weight ratio of the copolymer and the hemoglobin in the microcapsule is 1:3, and the particle diameter is less than 4 μm).

Embodiment 3

[0046] Dissolve 20mg 20,000 (1000) and 30mg 60,000 (10000) in 3ml of dichloromethane, add 600ul hemoglobin, 12000rpm for 30s, pour into 100ml of 2% glucose, PVA4% double emulsion, double-emulsify at 10000rpm for 1min, then pour into 200ml of 2% glucose aqueous solution, and stirred by magnetic force at 1000rpm under normal temperature and pressure for 1.5 hours. Finally, it was centrifuged and washed four times with 12000 g of normal saline, and the precipitated hemoglobin microcapsules were collected. (The weight ratio of copolymer and hemoglobin in the microcapsule is 1:2, and the particle size is less than 3 μm).

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Abstract

A haematoglobin microsoft capsule as the blood substitute contains the copolymer of one or more polylactic acids and monomethoxy polyethanediol and haematoglobin, and is prepared through dissolving said copolymer in organic solvent, dissolving haematoglobin in it, high-speed homogenizing or ultrasonic dispersing to generate W1 / O emulsion, adding it to re-emulsifier, high-speed homogenizing or ultrasonic dispersing to become W1 / O / W2 re-emulsion, adding to solidifying liquid, removing organic solvent, centrifugal washing and collecting haematoglobin microsoft capsules.

Description

field of invention [0001] The invention belongs to the field of biomedicine, in particular to a hemoglobin microcapsule blood substitute and a preparation method thereof. Background technique [0002] Nowadays, the shortage of clinical blood sources and cross-infection of blood have become a worldwide problem. Seeking safe and effective blood substitutes has become a research and development hotspot in the field of international medical biotechnology. [0003] The hemoglobin molecule is a tetramer composed of 2 α subunits and 2 β subunits, with a diameter of 5.5 nm and a molecular weight of 64,500 dal. Hemoglobin and O 2 The binding is reversible. The oxygen affinity of hemoglobin refers to the firmness of hemoglobin to oxygen binding, which is expressed by the partial pressure of oxygen required for the oxygen in hemoglobin to reach half-saturation, denoted as P 50 . The combination and release of oxygen by hemoglobin is a highly coordinated a...

Claims

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Application Information

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IPC IPC(8): A61K38/42A61P7/08A61P43/00
Inventor 苏志国孟凡涛赵东旭
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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