Blood substitute of hemoglobin microcapsule and preparing method thereof
A technology of blood substitutes and hemoglobin, which is applied in the field of hemoglobin microcapsule blood substitutes and its preparation, can solve the problems of low concentration of embedded hemoglobin, easy peroxidation of phospholipids, and poor stability, so as to improve the embedding rate and facilitate amplification , the effect of reducing particle size
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[0041] Example 1:
[0042] Dissolve 50mg 30,000 (5000) (PLA / MPEG molecular weight is 30,000, of which MPEG molecular weight is 5000, the same below) dissolved in 3ml dichloromethane, add 600ul bovine hemoglobin, colostrum at 10000rpm for 30s, pour 100ml NaCl 0.9%, PVA6 % Double emulsion, re-emulsify at 8000 rpm for 1 min, then pour it into 200 ml of physiological saline, and stir magnetically at 1000 rpm under normal temperature and pressure for 1.5 hours. Finally, centrifugation and washing four times with 10000g physiological saline to collect the hemoglobin microcapsule precipitation. (The weight ratio of copolymer and hemoglobin in the microcapsules is 1:2, and the particle size is less than 4 μm).
Example Embodiment
[0043] Example 2:
[0044] Dissolve 40mg 60 thousand (5000) in a mixed solvent of 2ml dichloromethane and 1ml ethyl acetate, add 800ul hemoglobin, colostrum at 10000rpm for 30s, pour into 100ml 1% glycerol, PVA6% double emulsion, re-emulsify at 8000rpm for 1min, Then pour it into 200ml of normal saline and stir it magnetically at 1000rpm under normal temperature and pressure for 1.5 hours. Finally, centrifugation and washing four times with 10000g physiological saline to collect the hemoglobin microcapsule precipitation. (The weight ratio of copolymer and hemoglobin in the microcapsules is 1:3, and the particle size is less than 4 μm).
Example Embodiment
[0045] Example 3:
[0046] Dissolve 20mg 20 thousand (1000) and 30mg 60 thousand (10000) in 3ml dichloromethane, add 600ul hemoglobin, 12000rpm colostrum for 30s, pour into 100ml 2% glucose, PVA4% double emulsion, re-emulsify at 10000rpm for 1min, then pour Into 200ml of 2% glucose aqueous solution, magnetic stirring at 1000rpm under normal temperature and pressure for 1.5 hours. Finally, it was centrifuged and washed four times with physiological saline at 12000g to collect the hemoglobin microcapsule precipitate. (The weight ratio of copolymer and hemoglobin in the microcapsules is 1:2, and the particle size is less than 3μm).
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