Preparation method of D-amino acid oxidase

Abstract

The preparation method of D-amino acid oxides is characterized by that is uses colibacillus gene engineering bacterium to make shake-flask strain culture in culture medium, fermentation strain culture and fermentation culture. Said invention is a preparation method which utilizes the colibacillus gene engineering bacterium to make fermentation production, utilizes centrifuge to collect thallus, soakes to extract enzyme liquor, and makes the enzyme liquor undergo the processes of plate-frame filtration, ultra filtration and concentration, purification and fixation so as to obtain the invented product.

Description

Technical field: [0001] The invention relates to a method for fermenting and extracting D-amino acid oxidase used for the key intermediate of semi-synthetic cephalosporin synthesis, 7-aminocephalosporanic acid. Background technique: [0002] Cephalosporins are antibiotics with important efficacy, while semi-synthetic cephalosporins have stronger antibacterial ability or can improve pharmacokinetic properties. And the key intermediate of semi-synthetic cephalosporin is 7-aminocephalosporanic acid (7-ACA), the method of traditional production in the past mainly is by chemical method, has four major steps, and process is complicated, and used reagent is expensive, and pollution is serious, And the yield is low and the quality is poor. The biotransformation method is a better method. Studies have shown that cephalosporin C can be oxidized and deaminated by D-amino acid oxidase, and further decarboxylated to generate GL-7-ACA, and finally generate 7-ACA, so D-amino acid oxidase...

AI Technical Summary

Problems solved by technology

And the key intermediate of semi-synthetic cephalosporin is 7-aminocephalosporanic acid (7-ACA), the method of traditional production in the past mainly is by chemical method, has four major steps, and process is complicated, and used reagent is expensive, and pollution is serious, And the yield is low, the quality is poor