Method for producing protease of thermophilic bacteria by using artificial gene
Patent Information
- Authority / Receiving Office
- CN · China
- Current Assignee / Owner
- FUDAN UNIV
- Publication Date
- 2003-10-08
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
technical field
[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for producing thermolysin by artificial gene. Background technique
[0002] Thermolysin was first isolated from Gram-positive bacteria Bacillusthermoproteolyticus Rokko (Endo, 1962). Due to its high heat resistance (when Ca 2+ In the presence of ions, it can stably exist at a high temperature of 80 degrees), so it is often used in some protein hydrolysis reactions that need to be carried out at higher temperatures. Thermolysin belongs to the M4 family of zinc endopeptidases and is the first endopeptidase to be crystallized.
[0003] Amino acid sequence (K.Titani, M.A.Hermodson, L.H.Ericsson, K.A.Walsh, andH.Neurath, Nature (London) New Biol., 1972,283,35) and three-dimensional structure (P.Mcolman, J.N.Jansonius) of thermolysin , and B.W.Matthews, J.Mol.Biol., 1972, 70, 701) have been determined. The active center of thermolysin is composed of zinc...