Wheat gibberella resistance major effect QTL linked molecular label and its application

A wheat scab and molecular marker technology, applied in the fields of application, botanical equipment and methods, plant gene improvement, etc., can solve the problems that there is no molecular marker yet, it is obviously better than Sumai 3, and has been used by breeders, etc. , to achieve the effect of improving accuracy and breeding efficiency and reducing workload

Inactive Publication Date: 2003-12-03
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0004] Wheat line Ning 894037 (Jiangsu Agricultural Science Journal, 1998, p129-134) is a somaclonal mutant obtained from Yangmai 3 in our laboratory through young panicle culture. The lev

Method used

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  • Wheat gibberella resistance major effect QTL linked molecular label and its application
  • Wheat gibberella resistance major effect QTL linked molecular label and its application

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Experimental program
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Effect test

specific Embodiment approach

[0018] Specific embodiments: materials and methods: 1. Ning 894037 / Alondra recombinant inbred line population construction and inoculation anti-red identification:

[0019] F 1 bastard, F 1 F 2 Offspring, F 2 The 219 individual plants randomly select a seed to plant, adopt the method of single-seed transmission, and propagate to F 8 generation, resulting in 219 families.

[0020] The identification of head blight resistance adopts the method of single flower dripping. The concentration of the conidia suspension of Fusarium graminearum is 50000 / ml. Select the ears that have just bloomed, and add 10ul of the spore suspension to the middle floret, and mist the greenhouse to keep it moist. For 3 days, in the field, bagging or artificial fogging was used to keep moisture. Each line was inoculated with 10 to 12 ears, and the rate of diseased spikelets was investigated 21 days after inoculation. Diseased spikelet rate=(number of sick spikelets / total spikelet number)×100%

[002...

Embodiment 1

[0024] Molecular markers linked to major QTLs for wheat head blight resistance were obtained by the following methods:

[0025] a) The wheat scab-resistant variety Ning 894037 (♀) was crossed with the wheat scab-susceptible variety Alondra (♂) to obtain the hybrid F 1 .

[0026] b) by Hybrid F 1 Self-pollination to obtain F 2 Multiple offspring, get F by single grain pass (SSD) method 8 generation of recombinant inbred lines.

[0027] c) Molecular marker analysis is performed on each strain of the recombinant inbred line, and the genotype of each strain of the recombinant inbred line is described; the specific method: separate the genomic DNA of each strain of the recombinant inbred line, and use SSR Primers carry out PCR amplification, amplified products are separated by electrophoresis on polyacrylamide gel of 6% (containing 5.7 gram acrylamide and 0.3 gram methylene acrylamide in 100ml polyacrylamide gel solution), after silver staining, obtain each strain genotype.

...

Embodiment 2

[0033] Using the screened molecular markers linked to the main QTL for scab resistance in Ning 894037, a preliminary test was carried out on the advanced breeding materials to test the practical value of this method in molecular marker-assisted selection for wheat scab resistance. Select 6 high-generation breeding materials or wheat varieties with Ning 894037 and its derivative varieties (lines) as male or female parents, isolate their genomic DNA, and then use SSR primers Xgwm533, BARC075 and BARC133 to perform PCR reactions on these DNA, electrophoresis Finally, determine whether there is a corresponding marker according to the size or presence of the DNA band. If there are 2-3 markers at the same time, it means that the resistance of the variety or strain to scab has reached the level of "resistance", and if it does not exist, it is susceptible , at the same time, it was verified by the results of inoculation identification and molecular marker detection of scab resistance. ...

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Abstract

A molecular marker associated with the major QTL of wheat resistance to head blight is disclosed. The SSR labelling technique is used to analyze the 894037/Alondra F4 recombinant selfing population. The existance of major QTL on the chromosome 3B of Ning 894037 is detected, and this can be used to explain 42.8% of head blight resistance. The obtained molecular markers Xgwm 533-130, BARC 133-115 and BARC 075-110 for the major QTL of Ning 894037 can be used to culture the disease-resistant wheat variety with high efficiency.

Description

technical field [0001] The invention relates to the field of wheat breeding and biotechnology, in particular to selecting and utilizing molecular markers linked to major QTLs for resistance to scab resistance in wheat for auxiliary breeding, so as to improve the efficiency of wheat scab resistance breeding. Background technique [0002] Wheat head blight caused by Fusarium graminearum (Schw) is a worldwide disease that seriously affects wheat yield and grain quality. It is also one of the important factors that plague small production in our country. In the winter wheat areas in the middle and lower reaches of the Yangtze River, South China winter wheat areas, and Northeast wheat areas, the affected area exceeds 100 million mu, accounting for a quarter of the total wheat area in the country. With global warming, changes in farming systems and methods, wheat redness The scope of mildew disease gradually expanded. In China, it is expanding to the Huaihai and Yellow River bas...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 任丽娟陆维忠沈晓蓉周淼平张旭马鸿翔
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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