Biological preparation for controlling parasitic nema of plant

A biocontrol agent, nematode technology, applied in the directions of plant growth regulators, biocides, animal repellents, etc., can solve the problems of high price, residue, groundwater pollution, etc., achieve stable control effect, easy industrial production, comprehensive properties Good results

Inactive Publication Date: 2004-02-25
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of nematodes mainly relies on chemical nematicides, but chemical nematicides are mostly destructive and highly toxic pesticides, which cause pollution and residues in groundwater, making their use subject to certain restrictions
At the same time, the chemical nematicides currently used in China are mainly imported products from abroad, and the price is too expensive

Method used

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  • Biological preparation for controlling parasitic nema of plant

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] Example 1: Biocontrol agents parasitize nematode eggs

[0031] 1. Preparation of test preparations

[0032] The GAUZ 412 strain was inoculated in a petri dish for culture, and the medium formula was 1000 ml of PDA medium with 6 grams of sucrose and 6 ml of glycerol, and after culturing at 25°C for 8 days, the spores were washed with sterile water, and the spores were washed with a hemocytometer. Count and make a spore content of 1×10 8 / ml of spore suspension.

[0033] 2. Test method

[0034] Take 5 milliliters of spore suspension with a pipette and place it in a sterile petri dish with a diameter of 5 cm, then insert the root-knot nematode egg (Meloidogyne) suspension, and inoculate 1000 eggs per dish.

[0035] Egg preparation: cut up the root system with a large number of root-knot nematodes and mix it with soil, then transplant 4-week-old tomato seedlings, and reproduce once every 5 months. When in use, take out the root system that has been inoculated with root-...

example 2

[0040] Example 2: Preparation of biocontrol agent by submerged fermentation and efficacy test on root-knot nematode

[0041] 1. Preparation of biocontrol agents by submerged fermentation

[0042] (1) Inoculate the hyphae or spores of GAUZ 412 on the slant of the solid medium of the test tube. The medium formula is 1000 ml of PDA medium, add 6 grams of sucrose and 6 glycerol, and cultivate it at 25 ° C for 8 days to obtain the test tube species.

[0043] (2) the slant seeds are inoculated into the triangular flask (500 milliliters) liquid medium, and the medium formula is to add 12 grams of molasses, 3 grams of ammonium sulfate in 1000 milliliters of PDA, 4 grams of sodium chloride, 1 gram of potassium dihydrogen sulfate, 2 g trisodium citrate, 8 g sucrose, 8 ml glycerin. At 26° C., cultured on a shaker (rotating speed 250 rpm) for 10 days.

[0044] (3) Insert the fermented triangular flask liquid strain into a 1 ton fermenter, the culture medium formula is the same as that o...

example 3

[0062] Example 3: Preparation of Biocontrol Agents by Solid Fermentation

[0063] Adopt aforementioned example two (1) to prepare solid test tube slant bacterial classification, example two (2) prepare triangular flask liquid bacterial classification, the triangular flask liquid bacterial classification that will cultivate is inserted in solid medium, and the formula of solid medium is 15% Bean cake powder, 20% bran, 55% crop straw bran, 10% crab shell powder or shrimp shell powder or chitin, cultured at 25°C for 20 days, dried at 42°C to make powder or granule.

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Abstract

The present invention relates to a biological control preparation for controlling plant parasite nematode. It makes said strain undergo the processes of solid or liquid culture to obtain somatic culture including hypha, conidium and chlamydospore, and mixes said somatic culture with crab shell powder, shrimp shell powder, chitin, active carbon or straw carbon and other auxiliary materials and adsorption carrier together according to a certain ratio, and then make them into powder or microgranules preparation for controlling plant parasite nematode.

Description

Technical field: [0001] The invention relates to a biocontrol preparation for preventing and treating plant parasitic nematodes, belonging to the technical field of biological pesticides. Background technique: [0002] Plant parasitic nematodes are a common plant disease worldwide. There are more than 70 known types of root-knot nematodes alone, which damage more than 3,000 plants and cause huge losses every year. Plant parasitic nematodes in my country mainly harm economic crops such as tobacco, flowers, vegetables, cotton, soybeans, peanuts, Panax notoginseng, American ginseng, etc. The losses caused by nematodes are astonishing. The global annual losses due to nematodes reach 100 billion US dollars (Weischer, 1994). At present, the control of nematodes mainly relies on chemical nematicides, but most of these chemical nematicides are destructive and highly toxic pesticides, which cause pollution and residues in groundwater, making their use subject to certain restrictions....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00
Inventor 周薇张克勤莫明和
Owner YUNNAN UNIV
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