Separation and purification process of lentinan

Abstract

The separation and purification process of lentinan uses the lentinan crude product as raw material and adopts the following steps: using hot water to make extraction, using micrometer-grade filter to make prefiltration, ultrafiltration membrane separation whose molecular weight trapping value is 5-100000 dalton, ultrafiltration membrane concentration whose molecular weight trapping value is 0.6-50000 dalton, using conventional filter to make end filtration, ethyl alcohol precipilation and drying so as to obtain the invented purified lentinan powder.

Description

technical field [0001] The invention relates to the extraction technology of lentinan, in particular to a process for separating and purifying lentinan from crude lentinan obtained by submerged fermentation. Background technique [0002] At present, Lentinus polysaccharide is widely used as a biological response modifier for viral hepatitis, low immune function, and In the treatment and adjuvant therapy of malignant tumors, etc. But most of the existing lentinan preparations used clinically, especially oral preparations, mostly have the following deficiencies: the content of lentinan in the preparations is too low, generally in the range of 15% to 28%, and the impurity components therein are too high. It makes its active ingredients neither pure nor stable, thereby affecting the therapeutic effect; meanwhile, the specificity of Lentinus polysaccharides in the preparation is too poor, and the molecular weight is not strictly controlled, ranging from 10,000 t...

AI Technical Summary

Problems solved by technology

But most of the existing lentinan preparations used clinically, especially oral preparations, mostly have the following deficiencies: the content of lentinan in the preparations is too low, generally in the range of 15% to 28%, and the impurity components therein are too high. It makes its active ingredients neither pure nor stable, thereby affecting the therapeutic effect; meanwhile, the specificity of Lentinus polysaccharides in the preparation is too poor, and the molecular weight is not strictly controlled, ranging from 10,000 to 1 million Daltons. The molecular weight of bacterial polysaccharides is above 100,000 Daltons, which is far from the average molecular weight of 40,000 to 90,000 Daltons required for clinical treatment. If the molecular weight is too small, the metabolism will be too fast, which is not conducive to resident absorption in the human body. If it is too large, the water solubility will become poor and it will not be easily absorbed by the human body, which will greatly reduce its curative effect on disease prevention

In order to solve the above problems, the Chinese invention patent specification whose authorized announcement number is CN1032428C discloses a new extraction process of lentinan or lentinan, which uses a single-stage ultrafilter to intercept and ethanol precipitation to extract lentinan, although it can improve The content of lentinan, but its yield is only 1-1.5%, and the molecular weight of lentinan, especially the large molecular weight lentinan, has not been controlled, and there are many invalid ingredients and impurities in it, and its quality and curative effect are still unsatisfactory.

The Chinese Invention Patent Application Publication No. CN1140202A provides a method for extracting lentinan for injection from the crude lentinan obtained by submerged fermentation. Although polysaccharides can also improve the content of lentinan, the equipment used in this method has a large volume, a large amount of solvent recovery, and the molecular weight of the obtained lentinan is not controlled, which will also affect its quality and curative effect.