Separation and purification process of lentinan

Abstract

The separation and purification process of lentinan uses the lentinan crude product as raw material and adopts the following steps: using hot water to make extraction, using micrometer-grade filter to make prefiltration, ultrafiltration membrane separation whose molecular weight trapping value is 5-100000 dalton, ultrafiltration membrane concentration whose molecular weight trapping value is 0.6-50000 dalton, using conventional filter to make end filtration, ethyl alcohol precipilation and drying so as to obtain the invented purified lentinan powder.

Description

technical field [0001] The invention relates to the extraction technology of lentinan, in particular to a process for separating and purifying lentinan from crude lentinan obtained by submerged fermentation. Background technique [0002] At present, Lentinus polysaccharide is widely used as a biological response modifier for viral hepatitis, low immune function, and In the treatment and adjuvant therapy of malignant tumors, etc. But most of the existing lentinan preparations used clinically, especially oral preparations, mostly have the following deficiencies: the content of lentinan in the preparations is too low, generally in the range of 15% to 28%, and the impurity components therein are too high. It makes its active ingredients neither pure nor stable, thereby affecting the therapeutic effect; meanwhile, the specificity of Lentinus polysaccharides in the preparation is too poor, and the molecular weight is not strictly controlled, ranging from 10,000 t...