Cosmetic composition material containing exogenous ligand-receptor system and its application
A technology for cosmetic compositions and coordination compounds, which can be applied to cosmetics, cosmetic preparations, and medical preparations containing active ingredients, etc., can solve the problems of adhesion of cosmetic active agents, fiber degradation, toxicity, etc., which are difficult to resist shampoo.
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Embodiment 1
[0296] Example 1: Shampoo resistance of anti-FITC-dextran complexes
[0297]In this example, anti-FITC (anti-fluorescein isothiocyanate) was adsorbed on the hair, and then the FITC-dextran complex was attached to the hair.
[0298] a) Anti-FITC adsorbed to the hair surface
[0299] Five hairs (ie, about 10 mg) from one strand of washed natural hair were placed as individual samples in siliconized glass flasks.
[0300] A 50 μg / ml anti-FITC (Sigma F-5636) solution was prepared in PBS buffer (0.1 M, pH 7.2). 800 μl of anti-FITC solution was brought into contact with the hair sample. The mixture was stirred at room temperature for 24 hours.
[0301] The sample was rinsed once with 1ml 0.05% Tween 20 PBS solution, then rinsed once with 1ml PBS solution, rinsed three times with distilled water (3×1ml distilled water vortexed for 1 minute)
[0302] b) Adsorption and immobilization of the FITC-dextran complex on the treated hair surface
[0303] A 100 µg / ml FITC-dextran (Sigma, ...
Embodiment 2
[0305] Example 2: Shampoo Resistance of Anti-FITC-Biotin Complexes
[0306] In this example, anti-FITC was adsorbed to the hair, and then the FITC-biotin complex was attached to the hair.
[0307] a) Anti-FITC adsorbed to the hair surface
[0308] (see Example 1)
[0309] b) Adsorption and immobilization of FITC-biotin complex on the treated hair surface
[0310] A 100 µg / ml FITC-biotin (Molecular Probes, ref: B-1370) solution was prepared in phosphate buffer (pH 7.2). Untreated hair and anti-FITC-treated hair were incubated in 1 ml of this solution for 2 hours at room temperature. Samples were rinsed 3 times with distilled water (3 x 1 ml distilled water vortexed for 1 min). Controls prepared in parallel corresponded to hair treated with anti-FITC but not exposed to FITC-biotin.
[0311] 50 μg / ml of avidin-peroxidase (Sigma, ref: A3151) was prepared in phosphate buffer (pH 7.2). The samples were incubated in 1 ml of this solution for 1 hour at room temperature and then ...
Embodiment 3
[0314] Example 3: Shampoo Resistance of Anti-FITC-Pigment Complexes
[0315] In this example, PEI-FITC (polyethyleneimine-FITC) was adsorbed to the hair, and then the anti-FITC-pigment complex was attached to the hair.
[0316] a) Preparation of PEI-FITC solution
[0317] 500 [mu]l of 100 mg / ml FITC (Pierce 46110) was prepared in DMF. 400 μl of FITC solution was gradually added to a small beaker containing 2 ml of 5% PEI (pH 8.5). The mixture was stirred at room temperature for 2 hours under the protection of an aluminum foil and then purified on a PD10 column (Amersham 17-0851-01) (mobile phase: 3.5 ml deionized water).
[0318] b) Adsorption of anti-FITC to the pigment surface
[0319] 100 mg ocher pigment (iron oxide) was washed with 1 ml PBS pH 7.2. 1 ml of anti-FITC solution at a concentration of 500 μg / ml in PBS pH 7.2 was added to the pigment. The mixture was stirred at room temperature for 2 hours and then centrifuged. Pigment-anti-FITC was washed 6 times with 1 ...
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