Process for producing peptide

A technology of target peptide and cystyl peptide, applied in the field of peptide preparation
CN1509336AInactive Publication Date: 2004-06-30SHIMADZU SEISAKUSHO CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHIMADZU SEISAKUSHO CO LTD
Publication Date
2004-06-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

An object of the present invention is to provide a method that can efficiently and mass-produce a target peptide by using a gene recombination method. The method of the present invention based on the combination of right-hand scissors (S-cyanation reaction) and left-hand scissors (cyanogen bromide treatment, enterokinase, factor Xa treatment, etc.) to cut out the target peptide and tandem repeat method can be used for large-scale synthesis of gene-based Peptides of recombinant technology, especially low molecular weight peptides.
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Description

technical field

[0001] The present invention relates to a method for preparing a target peptide or a salt thereof by stably expressing the target peptide in a microorganism in the form of a repeatedly linked precursor protein, and then breaking the peptide bond of the precursor protein. Background technique

[0002] There are three known methods for synthesizing peptides: an organic chemical method, a method using enzymes, and a method using genetic recombination technology.

[0003] Among them, it is very difficult to synthesize a peptide by a direct expression method in terms of a method using gene recombination technology. The reason is that even if the peptides can be directly expressed, the prepared peptides are rapidly decomposed one by one by the protease in the bacteria.

[0004] Therefore, when using genetic recombination technology to synthesize peptides, the fusion protein method of expressing them in the form of a fusion protein with a protective protein is gene...

Claims

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