Method of producing large quantity thick wall spore by thick wall spore verticillium liquid fermentation

A liquid fermentation and chlamydospore technology, applied in the field of mycology, can solve the problems of small production scale, difficult to realize industrialization, etc., and achieve the effect of great practical value

Inactive Publication Date: 2004-07-21
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In solid fermentation, more chlamydospores can be produced, but solid fermentation is difficult to realize industrialization and the production scale is small, which limits its application
The world's authority on the study of Verticillium chlamydospores, Kerry from the United Kingdom once instructed his students to conduct research on the production of chlamydospores by liquid fermentation, but there is still no breakthrough

Method used

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  • Method of producing large quantity thick wall spore by thick wall spore verticillium liquid fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of solid strains

[0023] Mix 100 grams of sweet potato powder, 75 grams of corn flour, 150 grams of bran, 150 grams of corncob, 5 grams of superphosphate, 5 grams of calcium carbonate, and 15 grams of bean cake powder, then add water to make the water 60%. Mix it with 40-mesh fine sand at a ratio of 1:0.2 (volume ratio), put it into a conical flask and sterilize it at 120°C for 40 minutes, then insert the cultured test tube species V.chlamydosporium ZK7, and cultivate it at 22°C for 18 days to obtain Solid bacteria.

[0024] 2. Inhibitor preparation (prepare 100 parts of inhibitor according to 10000 milliliters of culture medium)

[0025] 250 mg of 40% thiophanate-methyl, 450 mg of 50% carbendazim, and 1,000 mg of sodium diatomate, mixed well before use.

[0026] 3. Prepare the biocontrol agent of the present invention

[0027] Add 120 grams of molasses, 30 grams of ammonium sulfate, 40 grams of sodium chloride, 10 grams of potassium dihydrogen sulfat...

Embodiment 2

[0036] 1. The preparation of solid strains is the same as in Example 1.

[0037] 2. Inhibitor preparation (preparation of 100 parts of inhibitor according to 10000 milliliters of culture medium)

[0038] 100 mg of 40% thiophanate-methyl, 200 mg of 50% carbendazim, and 500 mg of sodium diatomate, mixed well before use.

[0039] 3. Prepare the biocontrol agent of the present invention

[0040] Add 120 grams of molasses, 30 grams of ammonium sulfate, 40 grams of sodium chloride, 10 grams of potassium dihydrogen sulfate, 20 grams of trisodium citrate, 80 grams of sucrose, and 80 milliliters of glycerin in 10000 milliliters of PDA. Then add the inhibitor formulated in the above 2, in the same way as in Example 1, culture after sterilization and inoculation, and observe the formation of chlamydospores by microscopic examination.

[0041] In addition, 500 milliliters of culture medium was prepared by using the above-mentioned liquid culture medium formula, and no inhibitor was adde...

Embodiment 3

[0045] 1, the preparation of solid strains is the same as in Example 1

[0046] 2. Inhibitor preparation (preparation of 100 parts of inhibitor according to 10000 milliliters of culture medium)

[0047] 400 mg of 40% thiophanate-methyl, 600 mg of 50% carbendazim, and 1500 mg of sodium diatomate, mixed well before use.

[0048] 3. Prepare the biocontrol agent of the present invention

[0049] Add 120 grams of molasses, 30 grams of ammonium sulfate, 40 grams of sodium chloride, 10 grams of potassium dihydrogen sulfate, 20 grams of trisodium citrate, 80 grams of sucrose, and 80 milliliters of glycerin in 10000 milliliters of PDA. Then add the inhibitor formulated in the above 2, in the same way as in Example 1, culture after sterilization and inoculation, and observe the formation of chlamydospores by microscopic examination.

[0050] In addition, 500 milliliters of culture medium was prepared by using the above-mentioned liquid culture medium formula, and no inhibitor was adde...

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Abstract

A process for greatly producing chlamydospores by liquid fermentation of verticillium chlamydosporium features that on the basis of previous technique, the solid bacterial strain instead of liquid one is used, and the depressant composed of thiophanate-methyl, carbendazim and sodium silicoalginate is added to create the condition benefiting growth of chlamydospores.

Description

Technical field: [0001] The invention relates to a method for producing a large amount of chlamydospores by liquid fermentation of Verticillium chlamydospores, which belongs to the technical field of mycology. Background technique: [0002] Plant parasitic nematodes are a common plant disease worldwide, causing huge losses to crops every year. In the biological control of nematodes, Verticillium chlamydospora is an important class of fungi. At present, nematode biological agents developed by using Verticillium chlamydospora have been marketed. The patent "A Microorganism and Its Method for Producing Biological Nematode Preparations" applied by the inventor in the early stage is to use Verticillium chlamydospora as the starting strain to develop a biological pesticide for preventing and controlling root-knot nematodes. The currently developed The product C. elegans Bic has been launched in large quantities. [0003] The ability to produce chlamydospores is a very important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04C12N1/14C12N1/38C12N3/00
Inventor 莫明和张克勤周薇
Owner YUNNAN UNIV
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