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Decolur yeast of manganese producing depended pervxidase and its application

A technology of peroxidase and yeast, which is applied in the fields of microbiology and environmental protection, can solve the problems of dye mineralization and easy loss, and achieve effective decolorization effect

Inactive Publication Date: 2004-07-21
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned aerobic bacteria all have the following disadvantages: 1. The dye cannot be completely mineralized; 2. It can only act on a few simple dye molecules (such as p-aminoazobenzene) (Chung and Cerniglia, 1992); 3. The specificity of the decolorizing enzyme is strong, and one kind of bacteria can only act on one kind of dye molecule; 4. The decolorizing gene is generally in the plasmid and is easy to lose
And so far there is no report about yeast producing lignin degrading enzymes and decolorizing dyes

Method used

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  • Decolur yeast of manganese producing depended pervxidase and its application
  • Decolur yeast of manganese producing depended pervxidase and its application
  • Decolur yeast of manganese producing depended pervxidase and its application

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Embodiment 1

[0046] The two yeast strains of the invention can effectively decolorize the dye culture solution containing 100-1000mg / L reactive black 5 within 24 hours, and can decolorize 100% of the dye culture solution below 300mg / L. (see accompanying drawings 5 ​​and 6).

[0047] These two strains of yeast can produce manganese-dependent peroxidase (MnP) under the condition that active black 5 exists, and the activity of this enzyme can reach 2000U / L or higher in culture fluid; See accompanying drawing 7 and Fig. 8 .

Embodiment 2

[0049] The two yeast strains of the invention also have obvious decolorization effects on other azo reactive dyes with azo bond and benzene ring structure and on anthraquinone dyes without azo bond. Debaryomyces polymorphus and Candida tropicalis under the condition of aerobic culture to the concentration of 100mg / L following several dyes: anthraquinone dye reactive blue RB19, azo dye reactive red (Reactive Red) M-3BE, azo dye reactive yellow (Reactive Yellow) ) M-3RE, azo dye reactive brilliant red (Reactive Brilliant Red) K-2BF, azo dye Procion Scharlach H-E3G, and the decolorization rate of azo dye Procion MarineH-EXL in 24 and 48 hours are shown in Fig. 9 and 10. However, the present invention is not limited to the decolorization and degradation of compounds.

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Abstract

A decoloring yeast to generate the Mn-dependent peroxidase used to decolor and degradate the industrial sewage generated by textile, printing and dyeing, and preparing dye is obtained from natural environment through selective culture. It includes Debaryomyces polymorphus (CGMCC No.0813) and candiba tropicalis (CGMCC No.0814).

Description

field of invention [0001] The invention belongs to the technical field of microbiology and environmental protection, in particular to two decolorizing yeasts producing manganese-dependent peroxidase and application thereof Background of the invention: [0002] Azo reactive dyes are currently the most widely used chemically synthesized dyes. Their molecular structures contain azo bonds and benzene rings, and there are many different substituent groups, which can form a wide range of color changes. At the same time, this type of dye is widely used in textile, paper and other industries because of its high-quality dyeing properties. A large amount of dye-containing wastewater produced by these industries has the characteristics of high toxicity, many changes in composition, and difficult biodegradation. Traditional biological treatment methods are difficult to play a role. As a major producer, consumer and exporter of dyestuffs in the world, the wastewater treatment of dyestuf...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/16C12N9/02C12N15/31D21C9/10
Inventor 杨敏杨清香韩云张书军李红岩
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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