Application of N-acetly glucosamine in the preparation of medicine for treating local injury and full body syndrome due to virus or bacterial infestation

Abstract

An application of N-acetylaminoglucose in preparing the medicines for treating the local injury and general sympton caused by virus or bacterium infection is disclosed. It has high curative effect up to 90%.

Description

technical field

[0001] The invention relates to the application of N-acetylglucosamine or pharmaceutically acceptable salt derivatives thereof in the preparation of medicines for treating local damage and systemic symptoms caused by bacterial and / or viral infections. Background technique

[0002] Symptoms of systemic poisoning caused by bacterial and / or viral infection, including endotoxemia and local damage caused by exotoxins, such as fever, headache, dizziness, delirium, nausea, vomiting, and general malaise. At present, there are mainly two methods for the treatment of systemic poisoning symptoms caused by bacterial infection: 1. antibacterial treatment; 2. supportive therapy.

[0003] Viral infections are mainly seen in coxsackie virus, ECHO virus, orthomyxovirus, paramyxovirus, adenovirus, coronavirus, reovirus, respiratory syncytial virus, rhinovirus, hepatitis A, B, C virus and Japanese encephalitis Viruses, causing fever and other systemic pois...

Examples

Embodiment 1

[0018] Embodiment 1. The wave-promoting test of the compound of formula (I)

[0019] 1. Test materials and methods:

[0020] 1.1 Sample: pure compound of formula (I).

[0021] 1.2 Test material:

[0022] Strains: Proteus mirabilis should meet the following biochemical reaction characteristics: power (+), urease (+), lactose (-), glucose (+), H 2 S (-), phenylalanine deaminase (+).

[0023] Medium: modified LB medium (composition: 1% tryptone, 0.5% yeast extract, 1% sodium chloride, 0.1% glucose, 0.002% TTC, pH7.2-7.4).

[0024] 1.3 Test method:

[0025] Control sample: plant Proteus mirabilis in the center of the LB plate and culture at 37°C for 9 hours;

[0026] Test sample: Add the compound of formula (I) at a final concentration of 0.5% to the LB plate, plant Proteus mirabilis in the same way, and culture at 37° C. for 9 hours.

[0027] 2. Test results and evaluation:

[0028] In the control sample, there are concentric rings that continuously expand outwards at inte...

Embodiment 2

[0030] The toxicological test of embodiment 2. formula (I) compound

[0031] Carry out the toxicology test of formula (I) compound, comprise:

[0032] 1. Acute toxicology test: including oral, intravenous injection and maximum dose administration test;

[0033] 2. Ames test;

[0034] 3. Mouse bone marrow cell micronucleus test;

[0035] 4. Mouse sperm teratogenic test;

[0036] 5. Chromosomal aberration test of mouse testis;

[0037] 6. Chronic lethal test;

[0038] 7. Subchronic toxicity (90-day feeding) test;

[0039] 8. Traditional teratogenicity test;

[0040] Test conclusion shows: when formula (I) compound acute toxicity test dose exceeds 2g / kg, acute poisoning reaction does not yet occur; In long-term toxicity test, the highest dose has reached 1g / kg, through four weeks test observation, no toxic reaction occurs; In the reproduction test, mice were fed from a conventional dose of 7 mg / kg, and after three passages, it was proved that the compound of formula (I) ha...

Embodiment 3

[0041] Example 3. Cytological test for regulation of microheterogeneic changes

[0042] The conventional incomplete 1640 medium was used for cell culture, and B16 tumor cells (purchased from Shanghai Institute of Cell Biology Tumor Cell Bank) were inoculated. In the continuous culture for more than 48 hours, under the condition that the waste of cell metabolism affects the growth environment, the changes of the micro-heterogeneity of the cells and the control effect of N-acetylglucosamine on them were observed. After adding N-acetylglucosamine with a final concentration of 1g / 100ml in the culture medium, the number of cells increased steadily with the prolongation of culture time during the growth process. Control test cells could not proliferate in the same medium and under the same culture conditions in the absence of N-acetylglucosamine. The test shows that under the condition of the compound of formula (1), the cells can adjust the micro-heterologous changes of the cells ...

technical field

[0001] The invention relates to the application of N-acetylglucosamine or pharmaceutically acceptable salt derivatives thereof in the preparation of medicines for treating local damage and systemic symptoms caused by bacterial and / or viral infections. Background technique

[0002] Symptoms of systemic poisoning caused by bacterial and / or viral infection, including endotoxemia and local damage caused by exotoxins, such as fever, headache, dizziness, delirium, nausea, vomiting, and general malaise. At present, there are mainly two methods for the treatment of systemic poisoning symptoms caused by bacterial infection: 1. antibacterial treatment; 2. supportive therapy.

[0003] Viral infections are mainly seen in coxsackie virus, ECHO virus, orthomyxovirus, paramyxovirus, adenovirus, coronavirus, reovirus, respiratory syncytial virus, rhinovirus, hepatitis A, B, C virus and Japanese encephalitis Viruses, causing fever and other systemic pois...

Examples

Embodiment 1

[0018] Embodiment 1. The wave-promoting test of the compound of formula (I)

[0019] 1. Test materials and methods:

[0020] 1.1 Sample: pure compound of formula (I).

[0021] 1.2 Test material:

[0022] Strains: Proteus mirabilis should meet the following biochemical reaction characteristics: power (+), urease (+), lactose (-), glucose (+), H 2 S (-), phenylalanine deaminase (+).

[0023] Medium: modified LB medium (composition: 1% tryptone, 0.5% yeast extract, 1% sodium chloride, 0.1% glucose, 0.002% TTC, pH7.2-7.4).

[0024] 1.3 Test method:

[0025] Control sample: plant Proteus mirabilis in the center of the LB plate and culture at 37°C for 9 hours;

[0026] Test sample: Add the compound of formula (I) at a final concentration of 0.5% to the LB plate, plant Proteus mirabilis in the same way, and culture at 37° C. for 9 hours.

[0027] 2. Test results and evaluation:

[0028] In the control sample, there are concentric rings that continuously expand outwards at inte...

Embodiment 2

[0030] The toxicological test of embodiment 2. formula (I) compound

[0031] Carry out the toxicology test of formula (I) compound, comprise:

[0032] 1. Acute toxicology test: including oral, intravenous injection and maximum dose administration test;

[0033] 2. Ames test;

[0034] 3. Mouse bone marrow cell micronucleus test;

[0035] 4. Mouse sperm teratogenic test;

[0036] 5. Chromosomal aberration test of mouse testis;

[0037] 6. Chronic lethal test;

[0038] 7. Subchronic toxicity (90-day feeding) test;

[0039] 8. Traditional teratogenicity test;

[0040] Test conclusion shows: when formula (I) compound acute toxicity test dose exceeds 2g / kg, acute poisoning reaction does not yet occur; In long-term toxicity test, the highest dose has reached 1g / kg, through four weeks test observation, no toxic reaction occurs; In the reproduction test, mice were fed from a conventional dose of 7 mg / kg, and after three passages, it was proved that the compound of formula (I) ha...

Embodiment 3

[0041] Example 3. Cytological test for regulation of microheterogeneic changes

[0042] The conventional incomplete 1640 medium was used for cell culture, and B16 tumor cells (purchased from Shanghai Institute of Cell Biology Tumor Cell Bank) were inoculated. In the continuous culture for more than 48 hours, under the condition that the waste of cell metabolism affects the growth environment, the changes of the micro-heterogeneity of the cells and the control effect of N-acetylglucosamine on them were observed. After adding N-acetylglucosamine with a final concentration of 1g / 100ml in the culture medium, the number of cells increased steadily with the prolongation of culture time during the growth process. Control test cells could not proliferate in the same medium and under the same culture conditions in the absence of N-acetylglucosamine. The test shows that under the condition of the compound of formula (1), the cells can adjust the micro-heterologous changes of the cells ...

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