Wheel leaf black algae engineering seedling fast breeding method
A black algae, engineering technology, applied in the biological field, can solve the problems of limited provenance, root damage, native habitat damage, etc., and achieves the effects of convenient operation, simple steps and low cost
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[0025] Example 1. Collect the provenance of Hydra verticillium from East Taihu Lake in Suzhou in November, cut the stem section with winter buds, soak in soapy water for 4h and rinse with tap water. Grow in a greenhouse in a 2L beaker. Tap water was changed daily and Hoagland nutrient solution was added once a week. The light intensity in the greenhouse is about 1000lx, the daily light is 10h, and the temperature is about 25°C. After 15 days, select 30 winter buds with strong stems and no defects in leaves, soak them in 70% ethanol for a few seconds, then soak them in NaClO bleach containing a certain amount of Tween-80 diluted in an appropriate ratio for a few minutes, rinse thoroughly with sterile water, Inoculate in a sealed Erlenmeyer flask containing 40mL 1 / 2Ms liquid medium, and cultivate in the tissue culture room. The average light intensity is 1500lx, the daily light is 10h, and the temperature is about 25°C. Thereafter, daily observation was performed, and contami...
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