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Wheel leaf black algae engineering seedling fast breeding method

A black algae, engineering technology, applied in the biological field, can solve the problems of limited provenance, root damage, native habitat damage, etc., and achieves the effects of convenient operation, simple steps and low cost

Inactive Publication Date: 2004-12-22
NANJING UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

During the salvage process, the root system is often damaged to a certain extent, and the individual plants are of different sizes, which is not conducive to their successful transplantation.
In addition, most of the natural sources of Hydrilla verticillium carry bacteria, which is not conducive to the safe use in fish, shrimp and crab ponds and indoor ornamental aquariums
Finally, H. verticillium mainly reproduces in winter buds, and the provenance in the natural environment is limited, and excessive collection will easily lead to the destruction of its original habitat.
[0004] So far, there have been no studies and reports on the cultivation of aseptic seedlings of Hydrilla verticillium and its large-scale artificial propagation at home and abroad

Method used

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Examples

Experimental program
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Effect test

example 1

[0025] Example 1. Collect the provenance of Hydra verticillium from East Taihu Lake in Suzhou in November, cut the stem section with winter buds, soak in soapy water for 4h and rinse with tap water. Grow in a greenhouse in a 2L beaker. Tap water was changed daily and Hoagland nutrient solution was added once a week. The light intensity in the greenhouse is about 1000lx, the daily light is 10h, and the temperature is about 25°C. After 15 days, select 30 winter buds with strong stems and no defects in leaves, soak them in 70% ethanol for a few seconds, then soak them in NaClO bleach containing a certain amount of Tween-80 diluted in an appropriate ratio for a few minutes, rinse thoroughly with sterile water, Inoculate in a sealed Erlenmeyer flask containing 40mL 1 / 2Ms liquid medium, and cultivate in the tissue culture room. The average light intensity is 1500lx, the daily light is 10h, and the temperature is about 25°C. Thereafter, daily observation was performed, and contami...

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Abstract

A high-speed reproduction method for the engineered black chara used for cleaning the polluted lake or for the culture of aquatic animals includes tissue culture and reproducing in large scale.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly producing high-quality engineering seedlings of Hydra verticillium. 2. Background technology [0002] Hydrilla verticillata Royle (Hydrilla verticillata Royle) is an aquatic herbaceous plant belonging to the Hydrilla family, which grows in freshwater lakes. The plant has a high tolerance to water pollution, and can be used as a water purification tool species and a vegetation restoration pioneer species in eutrophic water ecological restoration projects. The plant is also widely planted in fish, shrimp and crab ponds, which can not only provide bait for the above-mentioned aquatic products, but also provide them with necessary refuge and spawning places. In addition, the plant is also a commonly used landscaping material in the process of indoor ornamental aquaculture. [0003] At present, there is a great demand for H. verticillium seedlings on the market. H...

Claims

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Application Information

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IPC IPC(8): A01G33/00A01H4/00
CPCY02A40/80
Inventor 安树青周长芳蒋金辉尹大强
Owner NANJING UNIV
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