Mineralized fibrion/macromolecule composite porous material and preparation method

A technology of polymer materials and porous materials, applied in the field of mineralized silk protein/polymer composite porous materials and their preparation, can solve the problems of safety, high cost, complicated purification process, etc., and achieve low cost and good biological phase. Capacitive, simple process effect

Inactive Publication Date: 2005-01-12
TSINGHUA UNIV
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  • Abstract
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Problems solved by technology

The material contains collagen. At present, domestic medical collagen mostly relies on imports. In addition, the collagen purification process is complicated and the cost is high. However, the source of collagen produced abroad is mainly from animals, such as cattle and pigs, and there is a certain degree of safety. sexual problems

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  • Mineralized fibrion/macromolecule composite porous material and preparation method
  • Mineralized fibrion/macromolecule composite porous material and preparation method

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preparation example Construction

[0026] The preparation method of the framework material proposed in the present invention is mainly based on the mineralized silk protein material, and further compounding polymer materials to prepare the framework material for bone repair.

[0027] It mainly includes the following steps:

[0028] (1) Add a solution containing calcium ions dropwise in the silk protein solution, so that the mass percentage concentration of silk protein in the solution is finally 0.3%-30%, and the molar concentration of calcium ions is finally 0.01-2mol / L. Continuous stirring is required; continue to add phosphate ions dropwise to the solution in a molar ratio of Ca:P=1 to 2:1 under stirring, and finally adjust the pH value to 7.4 to 13;

[0029] (2) Stir the above solution and let it stand. After the precipitation and the supernatant are layered, remove the supernatant, centrifuge to separate the precipitation, wash repeatedly with deionized water until neutral, put the precipitation into a lyo...

Embodiment 1

[0037] 1) In the 500ml silk protein solution with a concentration of 0.9%, add dropwise 500ml of Ca(OH) with a concentration of 0.25mol / 2 After solution, continue to slowly add NaH with a concentration of 0.25mol / L 2 PO 4 The solution is 300ml, and the pH needs to be adjusted to 13 by constant stirring during the dropwise addition.

[0038] 2) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a lyophilizer to freeze dry, and then grind the dried product A dry powder was prepared.

[0039] 3) Weigh 1.0g PLA into a flask, add 10ml 1,4-dioxane to obtain a 10% solution, stir the mixture at room temperature for 2 hours, add 1.0g of the prepared mineralized silk protein, and mix well.

[0040] 4) Pour the mixed solution into a mold, freeze and freeze in a freeze dryer.

[0041]5) Demoulding the above-mentioned molding materials, washing...

Embodiment 2

[0043] 1) In the 900ml silk protein solution with a adjusted concentration of 33%, add 50ml of CaCl with a concentration of 0.2mol / L 2 After the solution, continue to slowly drop Na with a concentration of 0.2mol / L 2 HPO 4 Solution 50ml, mix well with a stirrer while adding dropwise. Slowly add NaOH solution dropwise to the solution in the first step above while stirring until the pH value is stable at 7.4.

[0044] 2) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a lyophilizer to freeze dry, and then grind the dried product A dry powder was prepared.

[0045] 3) Weighing 1.5g of sodium alginate, adding 1.0g of the prepared mineralized silk protein, and then mixing evenly.

[0046] 4) Pour the mixed solution into the mold, put the mold into 5% calcium chloride solution and cross-link for 12 hours, then remove the film, soak in ...

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Abstract

A mineralized porous silk protein/high-molecular material composition with high biocompatibility and biodegradability for repairing bone is prepared through preparing the aqueous solution of deglued silk protein, dropping the Ca ions contained solution in it while stirring, slowly dropping PO4 ions contained solution, regulating pH value, stirring, laying aside, centrifugal separation and washing several times, vacuum drying, grinding, and mixing with the biocompatible high-molecular material chosen from polylactic acid, copolymer of lactic acid and hydroxy acetic acid, sodium alginate, etc.

Description

technical field [0001] The invention relates to a mineralized silk protein / polymer composite porous material for bone repair and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Natural bone is a complex biomineralization system with a finely graded structure. Its organic components are mainly type I collagen fibers, and its inorganic components are mainly calcium phosphate salts and a small amount of carbonate apatite. Collagen molecules are regularly arranged in an array staggered by a quarter of each other to form collagen fibers, and form a periodic structure in which pore areas and overlapping areas alternate with each other, with a period of about 67 nanometers. Pore ​​regions provide mineral nucleation sites and regulate the orientation of minerals. Calcium-phosphorus salts in bone are mainly irregularly shaped flaky nanocrystals with a crystal thickness of 4-6 nanometers and a width of about 30-45 nanometers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44
Inventor 崔福斋孔祥东张伟
Owner TSINGHUA UNIV
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