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Method for trapping Empoasca vitis Gothe imago/nymph and Aleurocanthus spiniferus imago

A technology for the false-eyed green leafhopper and black thorn whitefly, which is applied to the device, application, animal husbandry and other directions of catching or killing insects, can solve the problem of not involving the attracting effect of tea tip volatile matter and the like, and achieves low cost and convenience. The effect of transportation and stable performance

Inactive Publication Date: 2005-01-26
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this tea leafhopper is not the Empoasca vitis Gthe we studied; [7] , yellow-green or yellow can also be subdivided into many types; moreover, this article does not involve the attracting effect of tea tip volatiles

Method used

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  • Method for trapping Empoasca vitis Gothe imago/nymph and Aleurocanthus spiniferus imago
  • Method for trapping Empoasca vitis Gothe imago/nymph and Aleurocanthus spiniferus imago

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Using adsorption method to separate and identify 9 kinds of volatiles from robust tea shoots: trans-2-hexenal, ocimene, cis-3-hexenyl ethyl ester, cis-3-hexen-1-ol , 3-hexenyl butyrate, linalool, 1-octanol, geraniol and indole

[0048] Make a hollow glass column with a small opening of 7 mm in diameter at both ends, with a volume of 5 liters. The inlet is connected to a compressed air bottle, and the outlet is connected to a Tenax TA adsorption column and a vacuum pump in turn. The TenaxTA column was activated with nitrogen at 275°C for 4 hours prior to adsorption of volatiles. Add 200 grams of strong tea tip into the glass container, blow in air, and draw air from the back of the adsorption column at the same time, control the flow with a flow meter before the inlet and behind the Tenax TA column, and the air inlet and outlet flow rates are both 300 ml / min.

[0049] After 12 hours, the adsorption column was removed, rinsed with distilled ether, and then slo...

Embodiment 2

[0050] Example 2: Determination of cis-3-hexen-1-ol, trans-2-hexenal and linalool as volatile components with attractant activity by bioassay

[0051] According to specific embodiment 2, using n-hexane as a solvent, 9 kinds of tea volatiles such as cis-3-hexenal, trans-2-hexenal, and linalool were formulated into solutions at a concentration of 1 μg / ml, as a taste source, using n-hexane as a control, using a Y-shaped olfactometer to measure the number of leafhopper adults, leafhopper nymphs, and whitefly adults towards the taste source. It was found that among the nine tested volatiles, the number of leafhopper adults, leafhopper nymphs, and whitefly adults tending to cis-3-hexenal, trans-2-hexenal and linalool, compared with the tendency of the control ( n-hexane) the difference between the number of insects reached a significant level (P<0.05).

Embodiment 3

[0052] Embodiment 3: cis-3-hexen-1-ol, trans-2-hexenal and linalool are made into flavor sources with a concentration of 0.01-20 μg / ml respectively, and then the three flavor sources are pressed 1: 0.2-1.5: 0.1-1 combination, prepared as attractant

[0053] Using n-hexane as a solvent, make cis-3-hexenal ethanol, trans-2-hexenal and linalool respectively into flavor sources with a concentration of 1 μg / ml, and then mix the three flavor sources at a ratio of 1:1 : 1 ratio combination, the bioassay by the "Y" shape olfactometer shows that the attracting effect is stronger than that of the single component. This is the attractant for the adults, nymphs and adults of the leafhopper and the adult blackleaf.

[0054] Soak rubber tips made of natural rubber in absolute ethanol for 24 hours and let dry. Soak such rubber head with attractant again for 24 hours, just made lure core.

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Abstract

The invention discloses a trap method for Empoasca vitis Gothe imago,nympha and spiny white fly imago, comprising the steps: 1)separating and authenticating 9 volatile matter from sturdy tea twig using adsorption method 2) determining three volatile ingredient with allure activity using biological determination method 3)respectively preparing three active ingredient, concentration 0.01-20mu g / ml, mixing according to 1:0.2-1.5:0.1-1, preparing attractant, 4)determining three colors with alluring effect to Empoasca vitis Gothe and spiny white fly from ten colors in the tea garden allure experiment, three colors being parrot green, bud green and jasmine yellow 5) combining attractant with bud green and jasmine yellow, making trapper for Empoasca vitis Gothe and spiny white fly The advantage of the invention : 1)no pollution, adapting to organic tea and nuisance free tea production, 2) low cost.

Description

technical field [0001] The invention relates to a method for trapping adult nymphs of the false-eyed green leafhopper and adults of the blackthorn whitefly in a tea garden. Background technique [0002] my country's tea garden area, output and export volume rank first, second and third in the world. Empoasca vitis Gthe and Aleurocanthus spiniferus (Quaintance) are important pests of tea trees. False-eyed green leafhopper belongs to Homoptera leafhopper family Cicadellidae, originally a polyphagous pest on weeds and woody plants. In the 1950s and 1960s, a large area of ​​land was reclaimed for tea plantation in my country. The leafhopper was transferred from host plants such as weeds and shrubs to tea trees, and gradually evolved into a dominant species. It is widely distributed in tea areas of my country and tea areas of neighboring countries such as Vietnam and Myanmar. The generations overlap, from the Qinling Mountains and Huaihe River region...

Claims

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Application Information

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IPC IPC(8): A01M1/20
Inventor 韩宝瑜董文霞崔林
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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