Moderate holophilic bacteria for degradation of phenylacetic acid and bacteria agent therefrom

A technology of phenylacetic acid and degrading bacteria, applied in the field of biological high, can solve the problems of wasting fresh water resources, low salt tolerance concentration, increasing treatment cost, etc., and achieves the effects of low production and use cost, good broad-spectrum, and good removal effect.

Inactive Publication Date: 2005-02-23
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Due to the double inhibition of microorganisms by the high concentration of inorganic salts and refractory toxic substances in saline wastewater, the previous biological treatment mode encountered the problems of low removal rate and low salt tolerance concentration
At present, most of the saline industrial wastewater in our country is untreated or diluted with a large amount of fresh water before treatment and discharge, which not only wastes a lot of fresh water resources, but also increases the treatment cost. Therefore, the research on the biochemical treatment of saline wastewater is of great significance It is very important, and the key to biochemical treatment is to obtain strains with both degradation ability and salt tolerance

Method used

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Examples

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Embodiment Construction

[0016] Examples of the present invention are described below.

[0017] Activate the original seed of the microorganism BYS-1 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prepared to inoculate seed tanks. The seed tank is 500 liters, the feeding amount is 400 liters, the medium formula is: glucose 0.8%, (NH4)2SO4 1%, K2HPO4 0.2%, MgSO4 0.05%, NaCl 1%, CaCO3 0.3%, yeast extract 0.02%, pH value 7.2-7.5. After the feeding is completed, 115°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultured shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultivate until the logarithmic growth phase, and the stirring speed is 220 rpm / min,...

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Abstract

A degrading bacteria BYS-1 for degrading phenylacetic acid under hyperhaline condition is disclosed. It is halomonas sp, belongs to moderate halophil. Its characteristics are G-, nemaline, (0.8-1.0)X(1.5-2.5)mum, flagellate, opal, semi-transparent. Contact enzyme and oxidase are positive, and it can hydrolyze starch and ferment glucose, reduce NO3- and growth under condition of 0-3.4M NaCl. It can be used in waste water biochemical treating system by phenylacetic acid.

Description

1. Technical field [0001] The invention discloses a moderately halophilic phenylacetic acid-degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, utilize microorganisms to degrade phenylacetic acid, and are suitable for biological treatment of modern industrial saline wastewater. 2. Technical background [0002] With the development of the economy, people pay more and more attention to the problem of environmental pollution. Water pollution is the top priority of environmental pollution. Saline wastewater occupies a large proportion of wastewater. It not only contains high salt content, but also generally contains a large amount of Refractory or toxic organic substances. Most of these substances are carcinogenic and teratogenic substances, and they are persistent in the environment for a long time. If they are discharged into the environment without treatment, they will accumulate in organisms through the enrichment of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 李顺鹏洪青何健陈立伟顾向阳
Owner NANJING AGRICULTURAL UNIVERSITY
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