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Reagent box and detecting method for nitrate and nitrite content of vegetables and fruits

A technology of nitrite, vegetables and fruits, which is applied in the direction of color/spectral characteristic measurement, material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, etc. Time and other issues, to achieve the effect of convenient storage and portability, fast detection speed and good reproducibility

Inactive Publication Date: 2005-05-11
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods require special equipment and professional testing personnel to operate, and the testing takes a long time. The measurement of a single sample takes 4 to 5 hours, the testing cost is high, and it is limited to testing in professional laboratories.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A kit for detecting the content of nitrate and nitrite in vegetables and fruits, which is equipped with a chromogenic plate with recesses, a plastic straw, a plastic cup, a small corner spoon, a bottle A containing a nitrate chromogenic agent, and a There are standard color cards of nitrite developer bottle B, nitrate 400mg / Kg, 600mg / Kg, 1200mg / Kg, 3000mg / Kg four color levels and nitrite 4mg / Kg one color level standard color card, in

[0045] The component and content of nitrate developer are (weight mg):

[0046] α-Naphthylamine 30

[0047] p-Sulfuran 10

[0048] Manganese sulfate 70

[0049] Barium sulfate 650

[0050] Zinc powder 20

[0051] Citric acid 220

[0052] The component and content of nitrite chromogen are (weight mg):

[0053] α-Naphthylamine 3

[0054] p-Sulfuran 15

[0055] Manganese sulfate 70

[0056] Barium sulfate 630

[0057] Citric acid 282

[0058] Weigh the above-mentioned components according to the amount, mix them thoroughly and fi...

Embodiment 2

[0060] The formula of nitrate color developer A of the present invention and nitrite color developer B is:

[0061] Nitrate developer A (weight mg):

[0062] Naphthaleneethylenediamine hydrochloride 10

[0063] p-Sulfuran 15

[0064] Manganese sulfate 90

[0065] Barium sulfate 490

[0066] Zinc powder 45

[0067] Citric acid 350

[0068] Nitrite developer B (weight mg):

[0069] Naphthaleneethylenediamine hydrochloride 43

[0070] p-Sulfuran 20

[0071] Manganese sulfate 90

[0072] Barium sulfate 480

[0073] Citric acid 367

[0074] In this embodiment, the nitrate color developer A and the nitrite color developer B are each formulated into 1000 mg according to the above formula, and the rest of the kit is the same as that in Example 1.

Embodiment 3

[0076] The formula of present embodiment nitrate developer A is (weight mg):

[0077] α-Naphthylamine 5

[0078] p-Sulfuran 15

[0079] Manganese sulfate 70

[0080] Barium sulfate 570

[0081] Zinc powder 20

[0082] Citric acid 320

[0083] The formula of nitrite developer B is (weight mg):

[0084] α-Naphthylamine 15

[0085] p-Sulfuran 20

[0086] Manganese sulfate 80

[0087] Barium sulfate 515

[0088] Citric acid 370

[0089] In this embodiment, the nitrate color developer A and the nitrite color developer B are each formulated into 1000 mg according to the above formula, and the rest of the kit is the same as that in Example 1.

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PUM

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Abstract

A kit comprises body, colour developing plate, plastic cup, and suction tube, spoon, colour developing reagent bottle A containing nitrate and bottle B containing nitrite, nitrate and nitrite standard colour card. The detection method includes dropping 1-2 drops of sample solution into cavity of colour developing plate to mix with 20-30 mg of developer, placing it for one minute and confirming nitrate and nitrite content in the sample by comparing its colour to the card for judging whether the content is overproof or not.

Description

technical field [0001] The invention relates to a device and a method for detecting the content of nitrate and nitrite, in particular to a kit for detecting the value range of nitrate and nitrite contained in vegetables and fruits and a detection method thereof. Background technique [0002] Nitrate itself is not harmful to the human body, but nitrate ingested in the body can be reduced to nitrite by bacteria in the gut. Excessive accumulation of nitrite in the human body can make the normal ferroglobin (Fe) in the blood 2+ ) into methaemoglobin (Fe 3+ ), the blood loses its oxygen-carrying function and causes hypoxia poisoning, resulting in methemoglobin prion; the human body can cause poisoning if it takes 0.3-0.5g of nitrite, and 3g can cause death. In addition, under acidic conditions, nitrite can form strong carcinogen N-nitrosamine compounds with secondary amine compounds in the intestine. Among the 120 nitrosamine compounds that have been disc...

Claims

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Application Information

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IPC IPC(8): G01N21/27G01N21/78
Inventor 华楚衍王钫陈健文朱加虹周根香
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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