Method for preparing fixed yeast cell micro capsule

A technology of immobilizing yeast and microcapsules, which is applied in the direction of fixing on/in the organic carrier, so as to reduce the leakage of yeast cells, ensure the effective utilization rate, and increase the yield

Inactive Publication Date: 2005-05-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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High-power high-voltage pulse...

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  • Method for preparing fixed yeast cell micro capsule
  • Method for preparing fixed yeast cell micro capsule

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example 1

[0022] Example 1: Please refer to figure 1 , the suspension culture yeast GS115 in the logarithmic growth phase was centrifuged to collect the bacteria, and mixed evenly with 15g / L sodium alginate solution. Under the action of a high-voltage electric field of a high-power microcapsule preparation instrument, drop into 100mmol / L calcium chloride solution for gelation reaction for 30 minutes to prepare calcium alginate gel beads with a particle size of 300μm, and then mix with 0.5g / L The mixed solution of polylysine and 5g / L chitosan was film-forming reaction for 10 minutes, 55mmol / L sodium citrate solution was liquefied for 10 minutes after washing with normal saline for 3 times, and polylysine was prepared after washing with normal saline for 3 times / Chitosan microencapsulated yeast with a thickness of 65 μm. The whole preparation process is completed under physiological conditions, and the survival rate of yeast cells is maintained at 100% during the preparation process. ...

example 2

[0023] Example 2: After the prepared polylysine and chitosan solutions used to prepare microcapsule membranes were fully contacted with yeast cells for 30 minutes, the dilution plate method was used to measure its effect on the activity of yeast cells. The results showed that the same as the control group. Compared with the number of live yeast cells after contacting with polylysine and chitosan, the number of live yeast cells did not decrease, which confirmed that polylysine and chitosan solutions had no effect on the activity of yeast cells.

example 3

[0024] Example 3: Place the prepared sodium alginate-polylysine / chitosan and calcium alginate microgel beads containing yeast cells in the YPD culture medium, culture in a shaker flask, 28°C, 180rpm, after 24 hours of cultivation (see figure 2 a and figure 2 b), Calculate the yeast cell leakage rate. The results showed that the leakage rate of cells in the sodium alginate-polylysine / chitosan microcapsule group was more than 3 times lower than that in the calcium alginate microgel beads group.

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Abstract

The present invention relates to immobilized yeast cell sodium alginate-polylysine/chitosan microcapsule preparing process. On the basis of original immobilized alginate cell, one great power microcapsule preparing instrument is adopted to make the sodium alginate solution with homogeneously mixed yeast cell under high voltage electrostatic field form homogeneously distributed liquid drops of controllable particle size in 100-500 micron and form micro bead with fixing calcium solution. The surface of the micro bead has one layer of microcapsule polylysine/chitosan film formed via ion complexing reaction, and calcium in calcium alginate gel is replaced with sodium citrate solution to form liquefied liquid environment inside the microcapsule. The present invention is prepared under mild completely physiological condition of pH 5.0-7.4, 4-30 deg.c and no shearing and vibration. The preparation process has yeast cell activity 100 % maintained and the culture process has cell leakage less than 10 %.

Description

technical field [0001] The invention relates to a method for preparing microcapsules, in particular to a method for preparing microcapsules with immobilized yeast cells. Background technique [0002] Yeast cell immobilization technology was mainly used in the brewing industry at first, but with the development of genetic engineering technology, the advantages of yeast cells as host cells for gene recombination are increasingly manifested. Therefore, the study of yeast cell immobilization has new application value. At present, alginate immobilization is widely used [Document 1: L. Kurillova, P. Gemeiner, A. Vikartovska, H. Mikova, M. Rosenberg and M. Ilavsky. Calcium pectate gel beads for cell entrapment. 6. Morphology of stabilized and hardened calcium pectate gel beads with cells forimmobilized biotechnology. J. microencapsulation, 2000, 17(3): 279-296]. But there are still many problems, such as calcium alginate particles are larger, which affects the diffusion of solutes...

Claims

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Application Information

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IPC IPC(8): C12N11/02C12N11/04
Inventor 马小军于炜婷雄鹰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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