Method for analyzing inclusion in cells based on microflow controlled chip and special chip

A microfluidic chip and analysis method technology, applied in biochemical equipment and methods, analytical materials, and microbial determination/inspection, etc., can solve the problems of difficult operation, low throughput, insufficient expansion, etc. control effect

Inactive Publication Date: 2005-06-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Capillary electrophoresis is a relatively mature method for analyzing the contents of single cells, but it has disadvantages such as difficult operation, low throughput, and not easy to be fully automated.
[0004] As a new type of analysis platform (also known as lab-on-a-chip, micro-full analysis system, etc.), microfluidic chip has the advantages of easy automation and high degree of integration. Currently, single-cell culture and cell separation have been realized on this platform. , sorting, and cell transportation, manipulation, and lysis, but its work in the analysis and detection of single-cell inclusions has not yet been fully developed worldwide

Method used

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  • Method for analyzing inclusion in cells based on microflow controlled chip and special chip
  • Method for analyzing inclusion in cells based on microflow controlled chip and special chip
  • Method for analyzing inclusion in cells based on microflow controlled chip and special chip

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Embodiment 1

[0025] Add 13 microliters of 10mMPBS buffer solution to the buffer pool and the waste pool successively. After observing under a microscope that each channel is continuously filled with buffer, insert electrodes into the buffer pool and the waste pool, and connect them to 500V and ground respectively. The crushing electrode in the sampling channel is connected to an AC voltage of 5V, thereby generating an electric field strength of 50V / cm in the radial direction of the channel. Add 13 microliters of carp red blood cell solution (the cell concentration is about 10 8 mL) in the cell pool. Cells flow towards the empty cell under static pressure. Cells are broken when flowing through the breaking electrode, and the released nucleic acids bind the buffer-weight intercalating dye. The dye used was YOYO-I (1uM). The laser wavelength is 488nm. After the nucleic acid flows into the cross area, it flows through the laser focal spot driven by the voltage between the buffer pool and t...

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Abstract

A method based on microcurrent controlled chip for analyzing the substances included in cell includes applying an AC field across two walls of a sampling channel on said microcurrent controlled chip to crack the cell, and electrophoretic separation and detection in the separation channel of chip. Said special chip is also disclosed.

Description

Technical field: [0001] The invention relates to a method for realizing analysis of cell content on a microfluidic chip. Background technique: [0002] The traditional method of analyzing cell inclusions is based on a large number of cells to obtain the total value of a certain inclusion (such as protein, nucleic acid, etc.), and the ratio of this value to the number of cells is extrapolated to the content of the substance in a single cell. For relatively homogeneous cell populations, this method is acceptable, but in other cases, such as the early stage of some serious diseases, only the composition of individual cells changes, at this time, the traditional method will average out. The specific change. Therefore, analysis at the single cell level is helpful for early diagnosis of diseases. In addition, single-cell analysis is relatively accurate in information acquisition compared with traditional methods; it is possible to detect unstable intermediates with shorter activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/447G01N33/50
Inventor 盖宏伟於林芬戴忠鹏马银法林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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