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Medical device with coating that promotes endothelial cell adherence and differentiation

A medical device and endothelial cell technology, which can be applied to devices of human tubular structures, medical preparations containing active ingredients, organic active ingredients, etc., and can solve the problems of cells losing endothelial cell function

Inactive Publication Date: 2005-06-15
祥丰医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microsurgery 18:263-256), most likely due to the failure of the cells to adhere properly to the graft and / or the loss of endothelial function of the cells due to ex vivo manipulation

Method used

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  • Medical device with coating that promotes endothelial cell adherence and differentiation
  • Medical device with coating that promotes endothelial cell adherence and differentiation
  • Medical device with coating that promotes endothelial cell adherence and differentiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0161] Phenotypic characterization of endothelial progenitor cells

[0162] A method for the isolation of putative endothelial cells for angiogenesis was recently described (Asahara T, Murohara T, Sullivan A et al. science , 1997; 275:964-967), endothelial progenitor cells (EPC) can be isolated by CD34+ magnetic bead separation method (Dynal Biotech) or enriched medium separation method. The method is generally as follows: obtain peripheral venous blood from healthy male volunteers, and separate the mononuclear cell composition by density gradient centrifugation. The cell material was plated in culture medium on culture slides (Bcton Dickinson) coated with human fibronectin. The medium was EC basal medium-2 (EBM-2) (Clonetics) supplemented with 5% fetal bovine serum, human vascular endothelial growth factor-A, human fibroblast growth factor-2, human epidermal growth factor , insulin-like growth factor-1 and ascorbic acid, the medium was changed every 48 hours, and the endot...

Embodiment 2

[0166] Capture of Inner Cells by Stainless Steel Disc Coated with Anti-CD34 Antibody

[0167] Human umbilical vein endothelial cells (HUVEC) (American Culture Collection) were grown in endothelial cell growth medium throughout the experiments. Cells were grown on samples coated with CMDX and gelatin with or without bound antibody on the surface, or on bare stainless steel (SST) samples. After incubation, the medium was removed and the samples were washed twice with phosphate buffered saline (PBS). Cells were fixed in 2% paraformaldehyde (PFA) for 10 minutes, and washed three times with phosphate buffered saline for 10 minutes each to ensure that the fixative was completely removed. Each sample must be incubated with blocking solution for 30 minutes at room temperature to block any non-specific blocking phenomena. After the samples were washed once with phosphate-buffered saline, they were exposed to VEGF receptor-2 antibody diluted 1:100 and incubated overnight. The samples...

Embodiment 3

[0172] Staining Effects of Vascular Endothelial Growth Factor Receptor-2 and Tie-2 on Progenitor Endothelial Cells

[0173] Progenitor cells were isolated from human blood as described in Example 1 and cultured in growth medium in vitro for 24 hours, 7 days, and 3 weeks. After incubation, the medium was removed and samples were washed twice with phosphate-buffered saline. The cells were then fixed with 2% paraformaldehyde (PFA) for 10 minutes, and then washed with phosphate buffered saline three times, 10 minutes each time. To ensure removal of all fixative components. Each sample was incubated with 440 microliters of goat (for VEGF receptor-2) or equine (for Tie-2) blocking solution for 30 minutes at room temperature. to block all non-specific binding. The samples were washed once with phosphate-buffered saline, then vascular endothelial cell growth factor receptor-2 or Tie-2 antibody diluted 1:100 with blocking solution was added, and the samples were incubated overnight....

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PUM

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Abstract

Various compositions and methods of producing a medical device are provided herein. Such medical devices include, for example, stents, stent-grafts, synthetic artificial blood vessels, heart valves, etc., all coated with biocompatible matrices containing various antibodies, antibody fragments, or small molecule substances Clothes. The biocompatible matrix has the ability to recognize, bind and / or interact with surface antigens of progenitor cells, thereby immobilizing said cells on the surface of the device. The coating material used for this device may also contain a compound or growth factor that can promote the process of adhesion, growth and differentiation of the progenitor endothelial cells on the surface of the device from a bound cell state to a mature one. and the ability of functional endothelial cells to prevent intimal proliferation. Methods are disclosed herein relating to such medical devices, their various components, and their use in patients with various vascular diseases, e.g., vascular valve restenosis, atherosclerosis, or other types of vascular disorders. Various methods of treatment for problem mammals.

Description

[0001] This application claims priority to US Provisional Patent Application Serial No. 60 / 354,680, filed 2002-02-06. [0002] scope of invention [0003] The present invention relates to various medical devices for implantation in blood vessels or hollow organs of the body. In particular, the present invention relates to various artificial medical devices placed in a cavity, the surface of which is in direct contact with blood, for example, various coated stents, stent grafts Medical devices such as stent grafts, synthetic artificial blood vessels, heart valves, catheters, and vascular prosthetic filters. The coating material on the implanted medical device can promote the adhesion, growth and differentiation of ancestral endothelial cells on the surface of the implanted medical device, and can form a functional endothelial cell layer, thereby inhibiting the Intimal proliferation of blood vessels and organs. Back...

Claims

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Application Information

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IPC IPC(8): A61M1/14A61BA61F2/82A61K31/721A61K39/395A61LA61L17/00A61L27/00A61L27/34A61L27/54A61L29/00A61L31/00A61L31/10A61L31/16A61M25/00A61M29/02
CPCA61K31/721A61L27/34A61L27/54A61L31/10A61L31/16A61L2300/256A61F2/07A61L29/04A61F2/24A61M25/00
Inventor M·J·B·库特里克R·J·小科托尼S·M·罗兰德M·A·库里泽斯基
Owner 祥丰医疗有限公司
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