Fluorogenic quantitative PCR method for detecting perforin gene expression in peripheral-blood under physiological state

A gene expression and perforin technology, which is applied in the fields of sports training and sports medicine, can solve the problems of lack of accurate and effective detection methods for the determination of immunity level.
CN1629312AInactive Publication Date: 2005-06-22SHANGHAI UNIV OF SPORT
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI UNIV OF SPORT
Publication Date
2005-06-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for performing quantitative determination to immunity-relating cytokine in peripheral blood, the method can be applied for specifically detect the cytokine gene expression (PFR) from 2ml of peripheral blood, and no any stimulation is needed before detection. The method can detect the cytokine gene expression under physiologic status, thus can be used for determining early stage fatigue, preventing decrease of body immunity function caused by exercises of excess sport load. The invention also provides a corresponding detection kit.
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Description

technical field

[0001] The present invention relates to the fields of physical training and sports medicine. Specifically, the present invention relates to a method for quantitatively detecting immune-related cytokines in peripheral blood. This method can specifically detect the gene expression of cytokines (perforin, referred to as PFR) directly from 2ml of peripheral blood, and without any stimulation before the detection, the expression of the above cytokine genes can be directly detected under the physiological state, thereby using It can be used to judge early fatigue and avoid the decline of immune function caused by excessive exercise load training. Background technique

[0002] Human helper T cells (Th) contain two subgroups that mediate cellular immunity and humoral immunity, namely Th1 and Th2. Th1 cells mainly secrete IFN-γ, IL-2 and TNF-α to regulate cellular immune function; Th2 cells mainly secrete IL-4, IL-5 and IL-10 to regulate humoral immune function. TH...

Claims

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