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Fluorescent rapid screening method for benzopyrene in food

A technology for food and benzos, which is applied in the field of fluorescence rapid screening of benzopyrene in food, can solve the problems of large sample loss and complicated experimental process, and achieves the effects of simple operation, low cost and reduction of pretreatment process.

Inactive Publication Date: 2005-06-29
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this detection method is a quantitative method recognized by the state, the experimental process is extremely complicated, and the sample loss is also large, which brings a lot of inconvenience to the quantitative work.

Method used

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  • Fluorescent rapid screening method for benzopyrene in food
  • Fluorescent rapid screening method for benzopyrene in food
  • Fluorescent rapid screening method for benzopyrene in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Sea oyster cake sample: After the sea oyster cake is ground, weigh 5g, wrap the sample in a filter paper, put it into a reagent bottle, soak it in 50mL of dichloromethane, let it stand, take out the supernatant with a straw, and transfer it into the fluorescence spectrophotometer. Conventional quartz fluorescence sample cell, for derivative-constant energy simultaneous fluorescence spectrum mapping. The instrument parameters are set as follows: constant energy difference Δv=1400cm -1 ; The scanning start excitation wavelength is 250nm, and the scanning stop wavelength is 600nm. get as figure 1 The constant energy synchronized fluorescence spectrum shown by figure 1 The benzo(a)pyrene peaks shown can be used for the identification and rough determination of benzo(a)pyrene.

Embodiment 2

[0036] Similar to Embodiment 1, the difference is that the second-order derivation function is added to obtain as figure 2 Derivative-constant energy synchronized fluorescence spectra shown. The signal intensity values ​​at 384 and 394nm of the spectrum were respectively read by the adjacent peak-valley method, and the sum of their absolute values ​​was the derivative fluorescence intensity for quantitative calculation. At the same time, take another 5 parts of the supernatant, add benzo(a)pyrene standard solution, and measure the derivative-constant energy synchronous fluorescence spectrum in the same way. Mapping such as image 3 standard addition curve. The linear fitting equation of the standard addition curve is Y=144.4+72.0*X, the correlation coefficient is 0.9992, and the linearity is good. Thus, the supernatant liquid contained 2.01 ng / mL of benzo(a)pyrene, which was converted into 20.1 μg / kg of benzo(a)pyrene in the sea oyster cake sample.

Embodiment 3

[0038] Shredded meat sample: cut the sample into pieces, grind it, weigh 5g, wrap it with filter paper, soak it in 25mL of dichloromethane solvent, place it, take out the supernatant with a straw, and transfer it to the conventional quartz fluorescence sample cell of the fluorescence spectrophotometer , for simultaneous fluorescence detection at constant energy. The instrument parameters are set as follows: constant energy difference Δv=1400cm -1 ; The scanning start excitation wavelength is 250nm, and the scanning stop wavelength is 450nm. get as Figure 4 The constant energy synchronous fluorescence spectrum shown can be used for the identification and rough determination of benzo(a)pyrene from the illustrated peak of benzo(a)pyrene. Adding the second-order derivation function, we get as Figure 5 Derivative-constant energy synchronized fluorescence spectra shown. The signal intensity values ​​at 384 and 394nm of the spectrum were respectively read by the adjacent peak-v...

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Abstract

This invention relates to fluorescence rapid filter of the benzopyrene in food, which comprises the following steps: to process sample, that is to shear and rub the food and dip them in the carrene and to take the upper liquid into the sample pool; to measure and to use the fluorescence spectrophotometer with derivative-constant energy simultaneously scanning; to add second derivative to be used in the said fluorescence spectrum; to map the constant energy simultaneously fluorescence spectrum or derivative-constant energy simultaneously spectrum; to read data ; to process data by use of standard sum method.

Description

technical field [0001] The invention relates to a screening method for benzo(a)pyrene, in particular to a rapid fluorescence screening method for benzo(a)pyrene in food. Background technique [0002] Benzo(a)pyrene (also known as 3,4-benzopyrene), as a kind of polycyclic aromatic hydrocarbons, is one of the strongest carcinogenic compounds known so far, and is often used as a typical representative of polycyclic aromatic hydrocarbons , is the most important food pollutant in more than 200 kinds of polycyclic aromatic hydrocarbons that have been found (Xu Mudan, Mao Gennian, Food Safety and Analysis and Testing, Beijing: Chemical Industry Press, first edition, 2003). Therefore, benzo(a)pyrene is the most commonly detected polycyclic aromatic hydrocarbon in food, and it is used as an indicator of polycyclic aromatic hydrocarbons to judge the pollution situation of polycyclic aromatic hydrocarbons. The source of benzo(a)pyrene in food is complex, and there are roughly the foll...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N21/31G01N21/64
Inventor 李耀群李呐林丹丽何立芳
Owner XIAMEN UNIV
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