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Nano microgel and its preparing process and use

A microgel and nanotechnology, applied in the direction of pharmaceutical formulations, etc., can solve the problems of inconvenient preparation, unstable system, no protein and polysaccharide to prepare microgels, etc., achieving good embedding effect, good edible absorption effect, The effect of broad application prospects

Inactive Publication Date: 2005-08-17
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drug / nutrient carriers formed by biomacromolecules still have the following disadvantages: they cannot effectively entrap, enrich and control release of charged hydrophilic small molecule drugs; At the micron scale; some systems are unstable and need to add stabilizers; the preparation is not easy
Although food proteins and polysaccharides can easily form macroscopic gels when heated, so far, no reports have been found on the use of proteins and polysaccharides to prepare microgels
In addition, there is no report on the preparation of nano-microgels using a simple heating method.

Method used

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  • Nano microgel and its preparing process and use
  • Nano microgel and its preparing process and use
  • Nano microgel and its preparing process and use

Examples

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Effect test

example 1

[0044] Example 1. Ovalbumin (Ovalbumin) and egg white lysozyme (Lysozyme) form microgels in alkaline aqueous solution.

[0045] Ovalbumin and egg white lysozyme were dissolved in deionized water to prepare 2mg / mL and 0.624mg / mL protein solutions. Under magnetic stirring, 1.2 mL of albumin aqueous solution was added dropwise to 3 mL of lysozyme aqueous solution, and the pH of the solution was adjusted to 10.3 with 0.1 mol / L NaOH and stirred for 60 minutes to make it evenly mixed. Then the mixed solution was placed in a water bath at 80°C and heated for 1.5 hours to obtain a stable microgel solution. Measure the obtained microgel solution with dynamic laser light scattering: the average particle diameter is 147nm, and the polydispersity coefficient is 0.127 ( Figure 4 ). The transmission electron microscope measured that the average particle size of the microgel was only 80nm ( image 3 ). The microgel solution is extremely stable, and there is no obvious change after 120 d...

example 2

[0048] Example 2. Ovalbumin (Ovalbumin) and egg white lysozyme (Lysozyme) form microgels in acidic aqueous solution.

[0049] Ovalbumin and lysozyme were dissolved in deionized water to prepare 2mg / mL and 0.624mg / mL protein solutions. Under magnetic stirring, add 600 μL of albumin aqueous solution dropwise to 3 mL of lysozyme aqueous solution, adjust the pH of the solution to 10.0 with 0.06mol / L NaOH and stir for 3 minutes to make it evenly mixed, and then quickly add 0.01mol / L HCl The pH of the solution was adjusted to 5.0, and then the mixed solution was quickly placed in a water bath at 80° C. and heated for 10 minutes to obtain a stable microgel solution. The obtained microgel solution was measured by dynamic laser light scattering: the average particle diameter was 71.4 nm, and the polydispersity coefficient was 0.224.

[0050] In the above system, if the molar ratio of albumin to lysozyme is controlled within the range of 0.05-5, and the pH of the solution is adjusted w...

example 3

[0051] Example 3. Bovine Serum Albumin (Bovine Serum Albumin) and lysozyme (Lysozyme) form microgels in alkaline or acidic aqueous solutions.

[0052] Dissolve bovine serum albumin and lysozyme in deionized water respectively to prepare 2.838mg / mL and 0.624mg / mL protein solutions. Under magnetic stirring, 1.5 mL of bovine serum albumin aqueous solution was added dropwise to 3 mL of lysozyme aqueous solution, and the pH of the solution was adjusted to 8.90 with 0.1 mol / L NaOH and stirred for 15 minutes to make it evenly mixed. Then the mixed solution was placed in a water bath at 80°C and heated for 1 hour to obtain a stable microgel solution. The obtained microgel solution was measured by dynamic laser light scattering: the particle size was 151.8 nm, and the polydispersity coefficient was 0.0880.

[0053] In the above system, the molar ratio of bovine serum albumin and lysozyme is controlled within the range of 0.05 to 5, and the pH of the solution is adjusted to the range o...

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Abstract

The present invention provides one kind of nanometer micro gel prepared with protein and polysaccharide and its preparation process, and belongs to the fields of biochemistry, high molecular chemistry and medicine preparation. The nanometer micro gel is formed with two kinds of protein or one kind of protein and one kind of polysaccharide differently charged in the same pH conditioned, and through heating to denature protein. Differently charged protein and polysaccharide forms electrostatic composite, micelle or deposit, and through pH regulation and heating, nanometer micro gel in core-shell structure is obtained. The nanometer micro gel may have medicine or nutriment coated through coating / diffusion to obtain nanometer edible medicine or nutriment colloid. Or, it may have perfume, cosmetics or dye coated for releasing in special environment.

Description

technical field [0001] The invention belongs to the fields of biochemistry, polymer chemistry and pharmaceutical preparations, and provides a protein / polysaccharide nano microgel. The invention also provides the preparation method and application of the nano microgel. Background technique [0002] Micro- and nano-sized drug carriers, which can be colloids, vesicles, or microspheres, have aroused widespread interest in the scientific and industrial communities. They can be loaded with living cells, enzymes, aroma oils, drugs, vitamins, agrochemicals, catalysts, etc., and have many advantages: liquids can be embedded in solids and used as solids; toxic substances can be handled safely; unstable Compounds can be protected; smell and taste can be effectively shielded; drugs can be controlled and selectively released, etc. (International Journal of Pharmaceutics 242 (2002) 163-166). In the food, pharmaceutical and cosmetic industries, drug / nutritional carriers formed from natur...

Claims

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Application Information

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IPC IPC(8): A61K47/36C08J3/075C08L37/00C08L89/00
Inventor 姚萍喻绍勇江明
Owner FUDAN UNIV
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