Process for preparing Danshensu

A technology of danshensu and salvia miltiorrhiza, applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of conflicting purity, yield and processing capacity, low processing capacity, high cost, etc., and achieve low cost and high recovery rate High, guaranteed separation performance

Inactive Publication Date: 2005-09-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF6 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adsorption resin and ion exchange resin method product quality is better (purity is more than 80%), but yield is only about 30%
Because the properties of water-soluble impurities are similar to those of Danshensu, and the amount is large, the puri

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1

[0032] 1. Crushing and degreasing

[0033] 2 kg of salvia root → crushed (1~3mm) → degreasing with petroleum ether (4L×3) → drying → defatted salvia miltiorrhiza powder.

[0034] 2: Extraction, ultrafiltration, concentration

[0035] Defatted salvia powder→16L deionized water extraction (80℃, 2 hours)→12L deionized water extraction (80℃, 2 hours)→8L deionized water extraction (80℃, 2 hours)→combination→ultrafiltration→decompression Concentrate to 2L.

[0036] 3: Conversion

[0037] Salvia miltiorrhiza concentrate → add sodium bisulfite (10g) and saturated NaOH to adjust to pH 9-10 → stir under nitrogen protection for 2 hours → add 2 mol.L -1 HCl to adjust the pH to 3.0.

[0038] 4: Extraction

[0039] Acidified Danshen transformation solution → ethyl acetate extraction (600 mL×5) → ethyl acetate recovery under reduced pressure → crude Danshensu (18 g, light yellow powder, Danshensu purity 65%, protocatechuic aldehyde 16%).

[0040] 5: Silica gel chrom...

Example Embodiment

[0042] Example 2

[0043] 1: Extraction, ultrafiltration, concentration

[0044] Salvia Root 100kg→Pulverization (1~3mm)→800L Deionized Water Extraction (80℃, 2 Hours)→600L Deionized Water Extraction (80℃, 2 Hours)→400L Deionized Water Extraction (80℃, 2 Hours) →Merge →Ultrafiltration →Concentrate under reduced pressure to 100L.

[0045] 2: Conversion

[0046] Salvia miltiorrhiza concentrate → add sodium bisulfite (500g) and saturated NaOH to adjust pH to 9-10 → stir under nitrogen protection for 2 hours → add 2 mol.L -1 HCl to adjust the pH to 3.0.

[0047] 3: Extraction

[0048] Acidified Danshen transformation solution→ethyl acetate extraction (30L×5)→recovery of ethyl acetate under reduced pressure→crude Danshensu (920 g, light yellow powder, Danshensu purity 67%, protocatechuic aldehyde 14%).

[0049] 4: Silica gel chromatography

[0050] Crude Danshensu (920g)→adding diatomite (2.5kg) and mixing→vacuum drying→solid powder→put on stainless steel column (silica gel 20k...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for preparing danshensu which comprises: getting extract liquid from the water or alcohol with danshensu and danshensu precursors using danshen as the materials, inverting the danshensu precursors of the said extract liquid into danshensu using diluted alkali, super filtering the macromolecular substance of the said extract liquid after conversion using a hyperfiltration membrane, extracting the danshensu from the said extract liquid after hyperfiltration, and separating the said extraction using the normal phase chromatography to get the danshensu.

Description

Technical field: [0001] The invention relates to a preparation method of medicinal danshensu, especially a preparation method whose process sequence is extraction→dilute alkali conversion→ultrafiltration→extraction→normal phase chromatography. Background technique: [0002] Salvia miltiorrhiza has the effects of expanding arteries, increasing coronary artery flow, improving myocardial anti-hypoxia ability, inhibiting platelet aggregation, anticoagulation, improving blood circulation, anti-oxidation, inhibiting endogenous cholesterol synthesis, anti-atherosclerosis and enhancing learning and memory. It is widely used in the prevention and treatment of diseases such as coronary heart disease, stroke, hyperlipidemia and peptic ulcer. Studies have shown that the water-soluble component Danshensu is its main active ingredient, and the clinical preparation is an injection with Danshensu as the main component. [0003] At present, Danshen injection is a brown clear liquid. In clin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C51/42C07C59/52
Inventor 杨凌何克江杨义
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap