Black aspergillus mutation of high active alpha-galactosldase and its ferment tech. on solid substrate

A technology of galactosidase and glucosidase Aspergillus niger, applied in the directions of enzymes, enzymes, biochemical equipment and methods, etc., can solve the problems of reducing the nutritional value of feed, retention of chyme, enlargement of the pancreas, etc., so as to improve the production of livestock and poultry. The effect of performance, low production cost and high enzymatic activity

Inactive Publication Date: 2005-11-09
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are high levels of α-galactosides in bean seeds and cake feed, which not only reduce the nutritional value of the feed, but also easily cause chyme

Method used

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  • Black aspergillus mutation of high active alpha-galactosldase and its ferment tech. on solid substrate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Bacteria culture:

[0028] 1, slant strain cultivation: Aspergillus niger varietal RM45# of the present invention is inoculated on the slant of Potato Ru glucose agar medium (PDA), cultivated at a constant temperature of 72-96 hours at 28-30° C., after growing plump yellow-brown spores , make spore suspension with sterile water (concentration is 10 7 -10 8 pcs / ml), for use.

[0029] 2. Solid seed culture: 1.5kg bran is added 1% (NH 4 ) 2 SO 4 The solution, after being thoroughly mixed, is made into a solid seed medium. The medium is sterilized at 121° C. for 30 minutes, and after cooling, 1-5% of the culture medium is added to the spore suspension of slant strains, fully stirred evenly, and 30 grams of water-containing and inoculated medium is filled into each 500 ml triangular flask.

[0030] 3. The inoculated solid seed culture medium is incubated at a constant temperature of 28-30°C for 72 hours. After the surface and inside of the medium grow plump yellow-...

Embodiment 2

[0046] 1. Bacteria culture:

[0047] 1, slant strain cultivation: Aspergillus niger variant RM45# of the present invention is inoculated on the slant of Potato Ru glucose agar medium (PDA), cultivated at a constant temperature for 96 hours at 28-30° C., after growing plump yellow-brown spores, use Spore suspension was prepared in sterile water (concentration of 10 7 -10 8 pcs / ml), for use.

[0048] 2. Solid seed culture: 1.5kg bran is added 1% (NH 4 ) 2 SO 4 The solution, after being thoroughly mixed, is made into a solid seed medium. The culture medium is sterilized at 121° C. for 30 minutes, and after cooling, 1-5% of the culture medium is added to the suspension of spores of slant bacteria, fully stirred evenly, and 30 grams of water-containing and inoculated culture medium is filled into each 500 ml triangular flask.

[0049] 3. The inoculated solid seed culture medium is incubated at a constant temperature of 28-30°C for 72 hours. After the surface and inside of th...

Embodiment 3

[0065] 1. Bacteria culture:

[0066] 1, slant strain cultivation: Aspergillus niger variant RM45# of the present invention is inoculated on the slant of Potato glucose agar medium (PDA), cultivated at a constant temperature of 72-96 hours at 28-30° C., after growing plump yellow-brown spores , make spore suspension with sterile water (concentration is 10 7 -10 8 pcs / ml), for use.

[0067] 2. Solid seed culture: 1.5kg bran is added 1% (NH 4 ) 2 SO 4 The solution, after being thoroughly mixed, is made into a solid seed medium. The culture medium is sterilized at 121° C. for 30 minutes, and after cooling, 1-5% of the culture medium is added to the suspension of spores of slant bacteria, fully stirred evenly, and 30 grams of water-containing and inoculated culture medium is filled into each 500 ml triangular flask.

[0068] 3. The inoculated solid seed culture medium is incubated at a constant temperature of 28-30°C for 72 hours. After the surface and inside of the medium g...

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Abstract

An Aspergillus niger variant (CGMCC No.1182) of the high-activity alpha-galactosidase is disclosed. It is suitable to grow in Cha's culture medium at 25 deg.C for 7 days and its spore shows tawny. It can be used to produce the high-activity alpha-galactosidase by solid fermenting. Its average enzyme activity can reach 286-345 IU/g, increasing it by 2.26 times.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a high-activity α-galactosidase Aspergillus niger variant and its fermentation process on a solid substrate. Background technique [0002] α-galactosidase is an enzyme that catalyzes the decomposition of α-galactosides into monosaccharides such as oligosaccharides, sucrose, and fructose, and is widely found in microorganisms, plants, and animals. α-Galactosidase has very important application value in all aspects of food and pharmaceutical industries. In the beet sugar industry, the raffinose in molasses is decomposed by α-galactosidase, which can not only increase the yield, but also improve the efficiency. In addition, it is also widely used in medicine and modification of polysaccharide gums. Studies in recent years have shown that this enzyme, as a specific exogenous feed enzyme, also has a broad application prospect in the feed industry. There are high levels of α-galactoside...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 许尧兴许少春姚晓红李孝辉范永仙
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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