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Method for promoting microbe to synthesize, 1,3-propylene glycol by adding fumaric acid from extraneous sources

A technology for fumaric acid and microbial synthesis, applied in fermentation and other directions, to achieve the effects of simple operation, reduced fermentation cost, and low additional investment

Active Publication Date: 2005-11-16
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current research, there is no report that exogenous addition of fumaric acid promotes the synthesis of 1,3-propanediol by microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] (1) Bacteria: Klebsiella pneumoniae M5a1

[0018] (2) culture medium

[0019] LB slant medium: every liter of medium contains 5.0 g of yeast powder, 10.0 g of peptone, 15.0 g of agar powder, and 10.0 g of NaCl, and the pH is adjusted to 7.0.

[0020] The ingredients of seeds and fermentation medium are shown in Table 1

[0021] Table 1

[0022] Medium components Seed medium Fermentation medium Trace element solution

[0023] ( / l) ( / l) (mg / l)

[0024] Glycerin 20g 20g ZnCl 2 70

[0025] K 2 HPO 4 ·3H 2 O 4.45g 2.225g MnCl 2 4H 2 O 100

[0026] (NH 4 ) 2 SO 4 2.0g 2.0g H 3 BO 3 60

[0027] K H 2 PO 4 1.3g 0.65g CoCl 2 ·6H 2 O 200

[0028] MgSO 4 ·7H 2 O 0.2g 0.2g NiCl 2 ·6H 2 O 25

[0029] Yeast powder 1.0g 1.5g NiCl 2 ·H 2 O 27.64

[0030] Trace element solution 2ml 2ml Na 2 MoO 4 2H 2 O 35

[0031] liquid

[0032] CaCO 3 2.0g CuCl 2 ·H 2 O 20

[0033] Defoamer 0.1ml...

example 2

[0045] (1) bacterial classification: with example 1

[0046] (2) culture medium: with example 1

[0047] (3) Training method:

[0048] A. Seed Activation

[0049] Pick one ring of bacteria preserved in the glycerol tube and transfer to LB slope for activation, and cultivate at 30°C for 12 hours to obtain activated seeds.

[0050] B. Seed culture

[0051] Seed culture adopts aerobic culture, using a 500ml Erlenmeyer flask with a liquid volume of 100ml. The culture temperature is 30°C, the rotation speed of the shaker is 140rpm, and the culture time is 14-16h.

[0052] C. Fermentation culture

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PUM

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Abstract

A process for preparing 1,3-propanediol by adding fumaric acid to activate microns includes preparing culture media, seed culture and fermenting. It features that the fumaric acid is added to the fermentive culture medium for speeding up the utilization of glycerine by bacterial spawn.

Description

technical field [0001] The invention belongs to the field of biochemical technology, and in particular provides a method for promoting the synthesis of 1,3-propanediol by microorganisms by adding fumaric acid from an external source. Background technique [0002] 1,3-Propanediol (PDO for short) is an important chemical raw material, which can be used as an organic solvent in ink, printing and dyeing, paint, lubricant, antifreeze and other industries. The most important use of 1,3-propanediol is as a monomer for polyester and polyurethane synthesis, especially polytrimethylene terephthalate (PTT), which is polymerized with terephthalic acid, shows a ratio of 1,2- Polymers synthesized from propylene glycol, butanediol, and ethylene glycol have better performance. At present, the world consumes tens of millions of tons of polyethylene terephthalate (PET) every year, and the chemical stability and biodegradability of PTT are comparable to PET, but the pollution resistance, toug...

Claims

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Application Information

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IPC IPC(8): C12P7/18
Inventor 刘德华林日辉刘宏娟
Owner TSINGHUA UNIV
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