Fluorescent capillary reaction device and fluorescent capillary analysis method

A technology of reaction device and fluorescence analyzer, which is applied in the directions of fluorescence/phosphorescence, material analysis by optical means, measurement device, etc., can solve the problem of inability to realize the fluorescence analysis of immobilized enzyme, and achieve the reduction and increase of fluorescence self-quenching phenomenon. Opportunity to be irradiated, the effect of increasing the light-receiving area

Inactive Publication Date: 2012-02-08
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] d). Using the test solution tank, only liquid enzyme fluorescence analysis can be performed, and immobilized enzyme fluorescence analysis cannot be realized

Method used

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  • Fluorescent capillary reaction device and fluorescent capillary analysis method
  • Fluorescent capillary reaction device and fluorescent capillary analysis method
  • Fluorescent capillary reaction device and fluorescent capillary analysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Utilize the present invention to riboflavin (VB 2 ) standard solution (S) concentration was determined. Measuring conditions: excitation light wavelength 500nm, emission light wavelength 550nm, room temperature 20°C, sample volume 10 μL. When measuring, place the fixed seat (5) of the fluorescent capillary reaction device in the light path of the fluorescence analyzer; use the capillary (7) to inhale VB with concentrations of 0.10, 0.50, 1.00, 1.50, 2.00, and 2.50 μg / L respectively. 2 After the standard solution, insert the capillary (7) into the insertion hole (6) on the top of the holder (5) respectively, and measure its fluorescence intensity, the results are as follows: Figure 4 shown. VB 2 There is a good linear correlation between concentration and fluorescence intensity in the concentration range of 0.10-2.50μg / L.

Embodiment 2

[0063] In this embodiment, the concentration of reduced coenzyme I (NADH) is determined by using the present invention. Measuring conditions: the wavelength of excitation light is 352nm, the wavelength of emission light is 456nm, the room temperature is 20°C, and the sample volume is 10 μL. When measuring, place the fixed seat (5) of the fluorescent capillary reaction device in the light path of the fluorescence analyzer; use the capillary (7) to inhale NADH standard solutions with concentrations of 50, 100, 150, 200, 250, and 350 μmol / L respectively , insert the capillary (7) into the insertion hole (6) on the top of the holder (5) respectively, and measure the fluorescence intensity, the results are as follows Figure 5 shown. There is a good linear correlation between NADH concentration and fluorescence intensity in the concentration range of 50-350μmol / L. With oxidized coenzyme I, coenzyme II (NAD + or NADP + ) as a coenzyme dehydrogenase reaction, the final product is...

Embodiment 3

[0065] This example is based on the determination of ethanol concentration by the immobilized enzyme FCA method. Measuring conditions: the excitation light wavelength is 352nm, the emission light wavelength is 456nm, the room temperature is 20°C, and the amount of the liquid to be tested is 10 μL. Before the measurement, the enzyme is fixed on the inner wall of the capillary (7) to make an ethanol capillary bioreactor. During the measurement, the fixed seat (5) of the fluorescent capillary reaction device is placed in the light path of the fluorescence analyzer; the ethanol standard solution and the reaction concentration of 2, 4, 8, 10, 15 g / L are inhaled respectively with the ethanol capillary bioreactor. After the mixed solution of the reagent is inserted into the insertion hole (6) of the fixed seat (5) respectively, the fluorescence intensity is measured, and the results are as follows: Figure 6 shown. There is a good linear correlation between ethanol concentration an...

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Abstract

The invention discloses a fluorescent capillary reaction device and a fluorescent capillary analysis method (FCA). The device includes a fixed seat, a capillary insertion hole, an excitation light incident window, a fluorescence emission window and a capillary; the device is used to implement the FCA method, and the fixed seat is placed in the optical path of the fluorometer, and then the capillary is sucked into the liquid to be measured and inserted into the fixed seat The capillary at the top is inserted into the hole, and the excitation light is irradiated into the capillary through the incident window of the fixed seat, and the liquid under test is excited to generate fluorescence, which is emitted from the emission window of the fixed seat, and reaches the detector through the monochromator to be detected. The present invention replaces the conventional test solution tank with a fluorescent capillary reaction device to form the FCA method, which can significantly reduce the amount of the test solution, save expensive reagents, and reduce waste; the inner wall of the capillary is fixed with different chemical reagents, enzyme preparations or DNA probes, which can form Dedicated capillary bioreactor, fluorescent capillary test box. The invention is applicable to the determination of the concentrations of various components in samples such as medicine, sanitation, industry, food and environment.

Description

technical field [0001] The invention relates to a fluorescent capillary reaction device and a fluorescent capillary analysis (FCA), belonging to the technical field of biochemical analysis and quantification. The invention is applicable to the fluorescence analysis of various trace components in trace samples of medicine, medicine, food, sanitation, industry, agriculture and forestry, environment and the like. Background technique [0002] When many substances absorb ultraviolet light and radiation in the visible spectral region, their electrons transition from the ground state to the excited state. When the electrons in the excited state return to the ground state, part of the energy is released in the form of thermal radiation, while the other part is in the form of light. This light is called fluorescence. The method of chemical analysis of unknown samples using fluorescence is called fluorescence analysis. [0003] The fluorescence analysis method has good selectivity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/01
Inventor 李永生高秀峰
Owner SICHUAN UNIV
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