A rapid detection method for polyhydroxy compounds based on the capillary siphon effect and the recognition principle of phenylboronic acid

A polyol compound and detection method technology, which is applied in the field of rapid detection of polyol compounds, can solve problems such as inability to obtain quantitative analysis results, and does not involve capillary siphon effect, and achieves the effect of low cost, low price, and less dosage

Active Publication Date: 2016-08-17
QUFU NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in these current analysis methods, the capillary is only the sample separation channel or the immobilization surface of the active substance (such as enzyme), and the suction of the sample still needs to use accessories such as flow injection or pump, which does not involve the siphon effect of the capillary. At the same time, It not only involves the limitations of the aforementioned active substances (such as enzymes), but also needs to rely on external signal detection instruments (such as fluorescence meters and electrochemical meters) to complete quantitative analysis, and quantitative analysis results cannot be obtained quickly and intuitively

Method used

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  • A rapid detection method for polyhydroxy compounds based on the capillary siphon effect and the recognition principle of phenylboronic acid
  • A rapid detection method for polyhydroxy compounds based on the capillary siphon effect and the recognition principle of phenylboronic acid
  • A rapid detection method for polyhydroxy compounds based on the capillary siphon effect and the recognition principle of phenylboronic acid

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Experimental program
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Effect test

Embodiment 1

[0049] Embodiment 1: the rapid measurement of glucose content in blood

[0050] 1) Capillary Surface Modification Functionalized Phenylboronic Acid Derivatives

[0051] Wash the inner and outer walls of the capillary with 2.0 mol / L NaOH-ethanol solution, then wash with ultrapure water, dry at 40 °C, and then use 6wt% hexadecyltrimethylsilane-ethanol solution and 6wt% Soak in 3-aminopropyltriethoxysilane-ethanol solution for 12 hours, take it out, wash it with absolute ethanol, dry it, then soak the capillary with 3wt% glutaraldehyde-water solution for 30 minutes, and then wash it with PBS buffer solution of pH 7. After drying, suck 4 mg / mL 3-aminophenylboronic acid-PBS buffer solution into the dried capillary, let it stand for 12 h, wash it again with PBS buffer solution of pH 7, and dry it to obtain the internally modified functionalized capillaries of phenylboronic acid derivatives;

[0052] 2) Quick test of blood sugar:

[0053] Insert the graduated capillary with intern...

Embodiment 2

[0055] Embodiment 2: Rapid detection of sugars in agricultural product samples

[0056] Taking the determination of sugars in stevia samples as an example, the ultrasonic-assisted water extraction method was used to dry the stevia samples of agricultural products in an oven at 50 °C for 10 h, grind them in a mortar, weigh 0.50 g accurately, and place them in a beaker , add 20 mL of ultrapure water, ultrasonically extract for 30 min, place it at room temperature, dilute to 25 mL, filter through a 0.45 μL microporous membrane to obtain a sample preparation solution, and prepare sample preparation solutions with different carbohydrate contents with PBS buffer Dilute to 1.0 mL to prepare a stevia sample solution with a concentration gradient of known sugar content.

[0057] 1) Capillary Surface Modification Functionalized Phenylboronic Acid Derivatives

[0058] Step is with embodiment 1;

[0059] 2) Rapid determination of sugars in stevia samples

[0060] Insert the scaled capi...

Embodiment 3

[0063] Embodiment 3: the rapid measurement of ethylene glycol content in chemical product

[0064] 1) Capillary Surface Modification Functionalized Phenylboronic Acid Derivatives

[0065] Step is with embodiment 1;

[0066] 2) Rapid measurement of ethylene glycol content

[0067] Insert the scaled capillary with aminophenylboronic acid modified inside into the ethylene glycol standard solution of known concentration gradient, and when the liquid level of the siphon in the scaled capillary no longer changes, record the height difference of the change in the level of the standard solution of the siphon in the scaled capillary , establish the standard curve of ethylene glycol concentration-liquid level difference and the quantitative equation y = 3.7363 + 0.0424 x (such as Figure 8 shown);

[0068] Insert the scaled capillary with aminophenylboronic acid modified inside into the ethylene glycol sample solution to be tested, and when the liquid level in the scaled capillary no l...

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Abstract

The invention relates to a polyhydroxy compound rapid detection method based on a capillary tube siphonic effect and a phenylboronic acid recognition principle. The method is a visual height rapid detection method established based on a specific recognition principle of a functionalization phenylboronic acid derivative to polyhydroxy compounds such as saccharides and the capillary tube siphonic effect. The method comprises the steps that the functionalization phenylboronic acid derivative is modified to the inner wall of a capillary tube, automatic suction of a sample is achieved through the capillary tube siphonic effect, a compound is formed through utilizing the phenylboronic acid on the wall and the polyhydroxy compounds such as monosaccharide with 1,2-glycol groups or 1,3-glycol groups, polysaccharide, epinephrine, ATP and dopamine, swelling of the compound happens, and the hydrophilic-hydrophobic property of the inner wall of the capillary tube is changed, so that the liquid level of the capillary tube with scales rises or declines regularly, and the content of the polyhydroxy compounds of a sample to be tested can be obtained through the liquid level of the sample in the capillary tube with the scales. The polyhydroxy compound rapid detection method based on the capillary tube siphonic effect and the phenylboronic acid recognition principle has the advantages of being little in dosage, low in cost, rapid, visualized, sensitive and the like.

Description

technical field [0001] The invention belongs to the field of chemical and biomedical detection, and relates to a rapid detection method for polyhydroxy compounds based on the capillary siphon effect and the recognition principle of phenylboronic acid. Background technique [0002] Polyhydroxyl compounds such as sugars (such as monosaccharides and polysaccharides) are important components commonly found in animals and plants, agricultural products and chemical products in nature, and have unique biomedical functions. For example, glucose is a kind of A monosaccharide with an aldehyde group, its concentration in human body fluids is an important indicator of human health, especially for diabetics, the monitoring of their blood sugar is very important. At the same time, polyols such as epinephrine, ATP, and dopamine containing 1,2- or 1,3-diol groups in the human body are also closely related to some major human diseases (senile dementia, Parkinson's disease, depression, etc.) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/00
Inventor 王桦张宁李征见陈丽君
Owner QUFU NORMAL UNIV
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