Purification process and application of antigen capable of being used in diagnosis of multiple myitis/dermatomyositis

A purification method and dermatomyositis technology, applied to the antigen purification process and application field that can be used for the diagnosis of polymyositis/dermatomyositis, can solve the problems of long time consumption, high cost, low yield, etc.
CN1740196AInactive Publication Date: 2006-03-01SINOGENOMAX +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SINOGENOMAX
Publication Date
2006-03-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to technological process of purifying Jo-1 antigen for diagnosis of multiple myitis / dermatomyositis. The technological process is specific combination of immunoaffinity chromatography and other protein purifying technology. The present invention also relates to the preparation process of Jo-1 immunoaffinity chromatography column, and Jo-1 antigen prepared through the said technological process and with proper biological tissue as material. The present invention also relates to the establishment of high efficiency immunoaffinity chromatography separation condition. The present invention also includes immunoassay kit prepared with purified Jo-1 antigen for clinical application and its application. In addition, the present invention provides one kind of technological way for preparing specific antigen used in detecting other autoimmune diseases.
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Description

technical field

[0001] The present invention relates to a Jo that can be used for the specific diagnosis of autoimmune diseases polymyositis (PM) / dermatomyositis (DM) and anti-Jo-1 antibody syndrome (Anti-Jo-1 Antibody Syndrome) -A convenient and efficient purification process for the 1 antigen, the Jo-1 antigen prepared by the process method is used to manufacture a clinically applicable polymyositis / dermatomyositis and anti-Jo-1 antibody syndrome specific diagnosis Immunoassay methods, such as the development of dot blot (DB) or enzyme-linked immunosorbent assay (ELISA) diagnostic kits, etc. Background technique

[0002] Polymyositis is clinically characterized by symmetrical muscle strength decline in varying degrees at the proximal extremities, and can also involve neck muscles and other skeletal muscles other than facial muscles, and most of them are accompanied by myogenic skeletal muscle enzymes in the blood. Damaged EMG changes, such as creatine kinase (CK), aldolas...

Claims

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