Method for measuring total flavone content in compositions extracted from gingko biloba leaves
A composition and a technology of total flavonoids, applied in the field of ginkgo biloba composition and its preparation, can solve the problems of harmful drugs, endangering the health of operators, and environmental threats, and achieve the effects of increasing safety, inhibiting allogeneic rejection, and reducing volume
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example 1
[0104] The invention provides a kind of method for preparing ginkgo composition, and the steps are as follows:
[0105] (a) Extraction: Ginkgo biloba green leaves are collected from late summer to early autumn. At this time, the content of effective therapeutic ingredients is high and the water content is lower than 8%. The 60% aqueous ethanol was refluxed twice under normal pressure, each time for 3 hours, the solid residue was removed by filtration and then refluxed for the third time with 10 times the volume of water for 0.5 hour, and the three filtrates were combined.
[0106] (b) Concentration: Concentrate the filtrate obtained in step a under reduced pressure to an alcohol-free viscous extract (density d=1.2-1.25, temperature t=60° C.).
[0107] (c) Precipitation: dissolve the viscous extract of step (b) with 2 times the amount of hot water, form a precipitate after cooling, and filter to remove the precipitate.
[0108] (d) column chromatography: the macroporous resin ...
example 2
[0115] The invention provides a method for preparing ginkgo composition, the steps are as follows:
[0116] (a) Extraction: Gather ginkgo green leaves in late summer and early autumn, when the content of effective therapeutic ingredients is high and the water content is lower than 8%, after drying, it is pulverized into leaves less than 4mm in a pulverizer. Then use 12 times the amount of solvent system to reflux twice, each time for 1 to 3 hours. The solvent system can be water, water-ethanol and acetone-water, filter to remove solids or residue, and combine the above two filtrates.
[0117] (b) Concentration: the filtrate obtained in step (a) is vacuum concentrated into an extract that does not contain organic solvents, (d=1.2~1.25, t=60°C)
[0118] (c) Precipitation: Dissolve the extract obtained in step (b) in 2 times the amount of boiling water, cool to about 12°C, a precipitate forms, and separate by filtration.
[0119] (d) Column chromatography: put the extract obtain...
example 3
[0123] The invention provides a method for preparing ginkgo composition, the steps are as follows: reflux 100kg of dry ginkgo leaf powder and 1000kg, 50% ethanol solution for 2 hours, filter, and then use 1000kg of filter residue, reflux with 50% ethanol for 2 hours, filter , combined two filtrates, concentrated in vacuum at 60° C. to no alcohol (d=1.2), dissolved the concentrate in 300 kg of water and left it for 48 hours, and filtered. The mixed column of macroporous resin and polyamide on the filtrate is respectively eluted with 100kg of water, 100kg of 30% ethanol solution, 100kg of 60% ethanol solution, and 100kg of 90% ethanol solution, and combines 30%, 60%, and 90% ethanol solution The aqueous solution was used to eluate, the combined solution was concentrated in vacuo to about 70 kg of extract, the extract was extracted three times with n-hexane, and the aqueous phase was concentrated and dried in vacuo. 1.5kg of the final product contains less than 5% water and 47.2%...
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