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Production bacterial strain of abamectin with high yield and high secreation rate and new method of extracting AVM

A high-secretion rate bacterium and abamectin technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve problems such as poor taste, and achieve good health, The effect of reducing the dosage and reducing the cost of refining

Inactive Publication Date: 2006-04-12
武汉天惠生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But without exception, a certain kind of traditional Chinese medicine is added to ordinary tea, so that it can achieve the purpose of treating a certain disease, but the taste of various Chinese medicinal materials is not very good.

Method used

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  • Production bacterial strain of abamectin with high yield and high secreation rate and new method of extracting AVM
  • Production bacterial strain of abamectin with high yield and high secreation rate and new method of extracting AVM

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Abamectin was produced by batch fermentation using Streptomyces avermitilis AVHF-31 strain.

[0089] The slant was prepared according to the formula of malt powder starch inorganic salt agar slant medium described above. After 3 days of pre-cultivation, if no bacteria growth was found, the AVHF-31 strain was inoculated. Cultivate in an incubator at 28°C for 5 days, harvest the spores after they mature, and store them in a refrigerator at 4°C for fermentation.

[0090] Prepare a 10L seed tank with a feeding factor of 65%. The ingredients of the seed medium are: 3% starch, 0.8% bean cake powder, 0.9% peanut cake powder, 0.45% yeast extract, 0.45% yeast powder, 0.3% corn steep liquor, 0.003% cobalt chloride, 0.06% defoamer, before disinfection Adjust the pH to 7.2 with sodium hydroxide solution. Steam sterilize at 121°C for 30 minutes, and inoculate the spore suspension of the AVHF-31 strain after the culture medium is cooled to 28°C. The amount of inoculation is to ino...

Embodiment 2

[0093] Abamectin was produced by batch fermentation using Streptomyces avermitilis AVHF-66 strain.

[0094] The slant was prepared according to the formula of malt powder starch inorganic salt agar slant medium described above. After 3 days of pre-cultivation, if no bacteria growth was found, the AVHF-66 strain was inoculated. Cultivate in a 27°C incubator for 7 days, collect the spores after they mature, and store them in a 4°C refrigerator for fermentation.

[0095] Prepare a 10L seed tank with a feeding factor of 65%. The ingredients of the seed medium are: 4% starch, 0.5% bean cake powder, 1.2% peanut cake powder, 0.3% active dry yeast powder, 0.5% corn steep liquor, 0.0015% cobalt chloride, 0.04% defoamer, and oxidized with hydrogen before disinfection Adjust the pH to 7.2 with sodium solution. Steam sterilize at 121°C for 30 minutes, and inoculate the spore suspension of AVHF-66 strain after the culture medium is cooled to 28°C. The amount of inoculum is to inoculate ...

Embodiment 3、4、5

[0098] Adopt exactly the same step as Example 1, just replace AVHF-31 bacterial strain with AVHF-27, AVHF-49 and AVHF-61 bacterial strain respectively and produce Abamectin by batch fermentation.

[0099] The yields of these three strains are shown in Table 1:

[0100] Example

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Abstract

A process for preparing abamectin (AVM) by use of the bacterium with high AVM secreting power includes such steps as mutagenizing the initial streptomyces avermitilis (CCTCC No.M200028), choosing the bacteria (CCTCC No.M204069 and CCTCC No.M204070) with high potency and high secretion rate, fermenting to obtain wax ball suspension of AVM, and extracting AVM by five-step method.

Description

【Technical field】 [0001] The invention relates to a novel production strain for fermenting and producing abamectin, and a new method for fermenting and extracting abamectin which is matched with it. 【Background technique】 [0002] Avermectin (AVM) is a class of macrolide antibiotics fermented by Streptomyces. It includes 8 chemical components with very close structures, called AVMA1a, AVMA1b, AVMA2a, AVMA2b, AVMB2a, AVMB2b. Among these eight components, AVMB1a has the strongest insecticidal and acaricidal activity. Therefore, abamectin products at home and abroad are currently AVMB1a products. The killing activity of AVMB1a against most harmful mites and pests in agriculture and forestry is several to dozens of times higher than that of general chemical pesticides, and the dosage in the field is only 0.015 grams to 1.5 grams per mu. The antibiotic is safe to most kinds of beneficial insects, has a short residual time in plants, and is quickly decomposed into non-toxic sub...

Claims

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Application Information

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IPC IPC(8): C12P19/62A01N63/00A01N25/12C12R1/465
Inventor 林开春朱时炎骆雪梅喻方春张用梅
Owner 武汉天惠生物工程有限公司
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