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A technology of therapeutic drugs and preventive drugs, applied in drug combination, food science, animal feed, etc., can solve unknown problems

Inactive Publication Date: 2006-06-21
TAKARA HOLDINGS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, insulin-like effects such as anti-diabetic effects and anti-obesity effects are still unknown

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The preparation of embodiment 1 long fruit kind jute extracting component

[0083] Add 40ml of distilled water, ethanol or ethyl acetate to every 2g of dried jute powder, extract at room temperature for 30 minutes, and centrifuge the extract and residue. Next, the extraction operation was repeated twice with 30 ml of the same solvent on the residue. The water extraction was carried out at 60°C, and the ethanol extraction and ethyl acetate extraction were carried out at room temperature. The resulting extract was collected and concentrated using a rotary evaporator. Finally, the water extract was dissolved in 20ml of distilled water to obtain the water extract fraction of C. longiflora jute. The ethanol extract was dissolved in 5ml dimethyl sulfoxide to obtain the ethanol extract fractions of C. jute. The ethyl acetate extract was dissolved in 5 ml of dimethyl sulfoxide to obtain the ethyl acetate extract fraction of C.

Embodiment 2

[0084] Example 2 The effect of long fruit seed jute extract components on differentiation induction into adipocytes

[0085] (1) Induction of differentiation into adipocytes

[0086] Induction of differentiation into adipocytes was carried out by partially improving the method of Rubin C.S. et al. (J. Biol. Chem., Vol. 253, No. 20, pages 7570-7578 (1978)). In Dulbecco's modified Eagle's medium (SIGMA, D6046) containing 200 μM ascorbic acid containing 10% fetal bovine serum (manufactured by GIBCO) (hereinafter referred to as A-D-MEM medium), 4 × 10 3 Each / ml suspended preadipocyte strain 3T3-L1 (ATCC CCL-92.1) was added to each well of a 12-well microtiter plate with 2 ml, and cultured at 37° C. for 7 days in the presence of 5% carbon dioxide. Medium exchange was performed with the same medium on days 2 and 4. On the 7th day, change to the A-D-MEM medium containing 0.25 μ M dexamethasone, then add the water extract component of the long-carved species jute prepared in Example...

Embodiment 3

[0090] Example 3 The promoting effect of long fruit seed jute extract components on glucose intake

[0091] (1) Preparation of mature adipocytes

[0092] Induction of differentiation into mature adipocytes was carried out by partially improving the aforementioned method of Rubin C.S. et al. In Dulbecco's modified Eagle's medium (manufactured by SIGMA, D6046) containing 200 μM ascorbic acid containing 10% fetal bovine serum, 4×10 33T3-L1 cells per ml were suspended, 2 ml were added to each well of a 12-well microtiter plate, and cultured at 37° C. for 7 days in the presence of 5% carbon dioxide. On the 7th day, it was replaced with 2 ml of 10% fetus containing 200 μM ascorbic acid, 0.25 μM dexamethasone, 10 μg / ml insulin, 0.5 mM 3-isobutyl 1-methylxanthine (manufactured by Nacalai Tesque, 19624-44). Dulbecco's Modified Eagle's Medium with Bovine Serum. After 45 hours, replace with 2 ml of Dulbecco's modified Eagle's medium containing 10% fetal bovine serum containing 200 μM ...

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Abstract

The present invention relates to a drug for treating or preventing diseases accompanied by abnormal insulin levels or insulin responses, an insulin-like agent, food, drink or feed for treating or preventing diseases accompanied by abnormal insulin levels or insulin responses, and an agent for promoting glucose uptake by cells , and an inducer that differentiates into adipocytes, characterized in that it contains at least A plant treatment is used as an active ingredient.

Description

technical field [0001] The present invention relates to a drug, food, drink or feed effective in the treatment or prevention of insulin-related diseases in vivo, such as diabetes and obesity. Background technique [0002] Insulin is a hormone necessary for normal carbohydrate, protein and fat metabolism in mammals. Type 1 diabetics cannot produce enough insulin, a life-sustaining hormone, and therefore require external administration of insulin in order to survive. Patients with type II diabetes have abnormal blood sugar levels due to insufficient insulin production, insulin resistance, etc., and in order to control the blood sugar level to an appropriate level, it is necessary to administer insulin or insulin secretion-stimulating drugs. However, among patients with type 2 diabetes, for those whose main cause is insulin resistance caused by hyperinsulinemia, insulin receptor abnormality, and insulin receptor downstream signaling abnormality, even if insulin or insulin secr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A23K1/16A23L1/30A23L2/00A61K36/185A61K36/28A61K36/282A61K36/287A61K36/70A61K36/704A61K36/886A61K36/896A61K36/906A61P3/10
CPCA61K36/185A61K36/28A61K36/282A61K36/287A61K36/70A61K36/704A61K36/886A61K36/896A61K36/906A61K36/9066A23K20/10A23L33/105A61P3/10
Inventor 榎龙嗣小川衣子大野木宏杉山胜美村木信子工藤庸子佐川裕章加藤郁之进
Owner TAKARA HOLDINGS
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