Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for separating and preparing triptolide diol from tripterygium wilfordii

A technology of lactone diol and triptolide, which is applied in the field of separation and preparation of triptolide diol, can solve the problems of unsuitable for large-scale separation and preparation of triptolide diol, cumbersome process, and high cost, and achieve low cost and wide distribution , the effect of little environmental pollution

Inactive Publication Date: 2006-07-12
ZHEJIANG UNIV
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing bibliographical reports separate and obtain the method for triptolide diol generally to adopt Tripterygium wilfordii extract through repeated column chromatography (Lin Sui et al., Acta Pharmaceutica Sinica, 2005, 40 (7): 632-635), and the process is cumbersome and time-consuming , also have literature (Wen-Zhen Gu, et al. International Journal of Immunopharmacology, 1995,17 (5): 351-356) to report adopting the preparation high-performance liquid phase to prepare and separate triptolide diol, is that each injection operation sample is few, The cost is high, and it is also reported that the preparation of triptolide diol by multidimensional countercurrent chromatography (Jun Wei et al., Journal of Liquid Chromatography & Related Technologies, 2005, 28: 1903-1911), but this method is not suitable for triptolide diol Large scale separation preparation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The root bark of Tripterygium wilfordii is extracted by ethanol, ethyl acetate, silica gel column chromatography, gradient elution with methanol-chloroform (3:97, 5:95, 10:90), collected step by step, and concentrated under reduced pressure to contain triptolide The diol part was evaporated to dryness and the resulting solid was used as the raw material. The raw material was extracted with a solvent system of n-butanol:ethanol:water=30:0.1:30 (v / v). First, prepare 0.5l solvent according to the above volume ratio and place it in a separatory funnel, add 10g of raw materials and shake for 3 minutes, let it stand for a period of time, separate the upper and lower phases, and then follow the above steps after the upper phase is concentrated under reduced pressure. Extracted 3 times, combined the lower phases and concentrated to obtain a solid. The obtained solid is dispersed in water, 100ml of ethyl acetate is added, extracted twice according to the ratio of ethyl acetate:...

Embodiment 2

[0017] The root bark of Tripterygium wilfordii is extracted by ethanol, ethyl acetate, silica gel column chromatography, gradient elution with methanol-chloroform (3:97, 5:95, 10:90), collected step by step, and concentrated under reduced pressure to contain triptolide The diol part was evaporated to dryness and the resulting solid was used as the raw material. The raw material was extracted with a solvent system of n-butanol:ethanol:water=30:0.2:30 (v / v). First, prepare 0.5l solvent according to the above volume ratio and place it in a separatory funnel, add 10g of raw materials and shake for 3 minutes, let it stand for a period of time, separate the upper and lower phases, and then follow the above steps after the upper phase is concentrated under reduced pressure. Extracted 3 times, combined the lower phases and concentrated to obtain a solid. The obtained solid was dispersed in water, 100ml of diethyl ether was added, extracted twice according to the ratio of diethyl ethe...

Embodiment 3

[0019] The root bark of Tripterygium wilfordii is extracted by ethanol, ethyl acetate, silica gel column chromatography, gradient elution with methanol-chloroform (3:97, 5:95, 10:90), collected step by step, and concentrated under reduced pressure to contain triptolide The diol part was evaporated to dryness and the resulting solid was used as the raw material. The raw material was extracted with a solvent system of n-butanol:ethanol:water=35:0.1:35 (v / v). First, prepare 0.5l solvent according to the above volume ratio and place it in a separatory funnel, add 10g of raw materials and shake for 3 minutes, let it stand for a period of time, separate the upper and lower phases, and then follow the above steps after the upper phase is concentrated under reduced pressure. Extracted 3 times, combined the lower phases and concentrated to obtain a solid. The obtained solid is dispersed in water, 100ml of ethyl acetate is added, extracted twice according to the ratio of ethyl acetate:...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for separating preparing for triptolide ethylene alcohol from Tripterygium wilfordii. It uses the Tripterygium wilfordii velamen extraction of triptolide ethylene alcohol as raw material and uses mixing reagent to eject a plurality of times to obtain the solid substance which uses organic solution to eject a plurality of times, it collects the organic phase and evaporate to dryness to obtain the solid substance, and then it coats primary silica gel column or normal alumina column and uses carrene, chloroform or dichloromethane to clean it to obtain the triptolide ethylene alcohol course product, and then it uses carreen-ether crystallization to obtain the triptolide ethylene alcohol coarse-grain, it obtains the purifying grain by re-crystallizing two times.

Description

technical field [0001] The invention relates to a method for separating and preparing triptolide diol from tripterygium wilfordii. Background technique [0002] Tripterygium Wilfordii (Tripterygium Wilfordii Hook.f.), a plant of the genus Tripterygium Wilfordii, has 4 species in the world, 4 species produced in China, and 3 species used as medicine (Edited by Li Ruilin et al., Research and Clinical Application of Tripterygium wilfordii, first edition , Beijing, China Science and Technology Press, 1989: 11-12). Because Tripterygium wilfordii has significant physiological activities such as anti-inflammation, immunity, anti-tumor, anti-cancer, and anti-fertility, it has been widely used in the treatment of diseases such as rheumatism, skin disease, and kidney disease (Zhou Xue-ping et al., Journal of Traditional Chinese Medicine (Engl.), 2004, 24(2): 83-87; Hua Guo et al., World Journal of Gastroenterology (Engl.), 2003, 9(7): 1550-1553; Wang Li-Ling et al. , Chinese Journal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/14C07D493/12
Inventor 何潮洪欧阳小琨喻均
Owner ZHEJIANG UNIV