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Method for separating vindoline , catharanthine, vinblastine and vincristine from Vinca rosea

A technology of vinblastine and vincristine, which is applied in the field of producing high-purity vinblastine alkaloids, can solve the problems of long extraction time of vinblastine, low extraction rate and yield, difficulty in popularization, etc., achieves less solvent consumption, Fully utilized, high-purity effects

Inactive Publication Date: 2006-07-12
NORTHEAST FORESTRY UNIVERSITY +1
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AI Technical Summary

Problems solved by technology

[0006] The above-mentioned production method of vinblastine generally has the disadvantages of longer extraction time, lower extraction rate and yield, high production cost, complicated procedures and not easy to popularize.

Method used

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Examples

Experimental program
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Embodiment 1

[0024] Get new periwinkle 100kg (moisture content 80%) and be divided into 25 equal parts, be placed in the beater respectively, add the pre-prepared nutrient solution 10kg (the nutrient solution formula is: sodium acetate 0.02 μ mol / L, ethephon 1mg / L, Abscisic acid 0.2mg / L, L-tryptophan 25μmol / L, acetylsalicylic acid 15μmol / L, hemoglobin 50μg / L, heme 50μg / L, hydrogen peroxide 0.15mmol / L, phenylalanine 3mg / L, jasmine methyl ester 0.25 μmol / L, horseradish peroxidase 0.0065 μmol / L), the refining time is 3 minutes, and the final particle size of the material is 0.02-8 μm. Then pour it into a culture tank with a filter membrane, use negative pressure 0.06-0.1MPa suction or positive pressure 0.1-0.6MPa air intake to carry out homogenate culture every 2h, and each culture time is 10min. After culturing for 24 hours, remove the culture medium, mix the remaining material with 100kg of 70% ethanol extractant and put it into a homogenizer for homogenate extraction, negative pressure cav...

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PUM

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Abstract

The invention relates to a method for separating catharanthine, eurosine and vincristin, which comprises: expelling the foreign matter of Catharanthus roseus, homogenate training the mushrooms, expelling the training liquid, hollowing the turbid liquor, ejecting the liquid, filtering, the extracting liquid negative pressure forming film condensation, double-water phase extracting, extracting liquid condensation, Catharanthus roseus total alkaloid, continues middle compression leg colour printing gradient cleaning to obtain the catharanthine, eurosine and vincristin. The eurosine uses negative pressure mold method to obtain the eurosine with high purity.

Description

Technical field: [0001] The present invention relates to a kind of method for separating mandolin, vinblastine, vinblastine and vincristine from periwinkle, in particular to a method for producing high-purity Process method of vinca alkaloids. Background technique: [0002] Currently, vinblastine and its analogues are generally extracted from Catharanthus roseus, a plant of the family Apocynaceae. [0003] The current extraction methods of vinca alkaloids are: [0004] 1. A new process for extracting anti-cancer active ingredients from periwinkle, China Patent Office Publication No.: CN1365978a; Publication Date: 2002.08.28; Application No.: 011319879.9. This invention relates to extracting vinblastine, The process method of vincristine and ursolic acid is to use periwinkle dry material grinding → benzene percolation → 3-10% tartaric acid liquid countercurrent extraction → acid soluble matter separated by column chromatography → vinblastine and vincristine, from Ursolic a...

Claims

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Application Information

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IPC IPC(8): C07D519/04C07D471/18C07D471/16
Inventor 祖元刚罗猛牟璠松唐中华赵修华祖柏实张琳田浩付玉杰张宝友
Owner NORTHEAST FORESTRY UNIVERSITY
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