Compound hemostatic originated from venom
A hemostatic drug and snake venom technology, applied in the field of enzyme preparations, can solve problems such as glomerular damage, adverse immune response, and limited dosage, and achieve remarkable drug effects
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Embodiment 1
[0040] Example 1 coagulation factor X activator (FXA) in vitro coagulation test
[0041] The method for measuring the activity of similar products was used for the in vitro coagulation test. The specific test method was as follows: take 0.2ml of human-citrate anticoagulant plasma, put it into a test tube with a diameter of 1cm, put it in a water bath at 37°C for 3 minutes, and add it to preheat at 37°C Mix 0.2ml of the sample solution immediately, start at 40 seconds, check the plasma coagulation situation, record the initial coagulation time, measure 3 tubes at the same time, the error of the initial coagulation time of the three tubes should be less than 20 seconds. If the initial coagulation time is less than 40 seconds, dilute the test solution appropriately, and record the concentration of the test solution coagulated within 60 ± 20 seconds. The amount of enzyme coagulated, defined as one unit. Calculate the potency and record the test results.
[0042]
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Embodiment 2
[0046] Example 2 Class thrombin (batroxobin) and coagulation factor X activator synergistic in vitro coagulation test
[0047] Use the thrombin-like enzyme (batroxobin) with a concentration of 500u / ml and the coagulation factor X activator with a concentration of 500u / ml to prepare the test solution according to the required ratio in the table below to ensure that the theoretical value of the final solution is: 500u / ml. Positive control 1 was 1 unit of thrombin-like enzyme (batroxobin), positive control 2 was 1 unit of coagulation factor X activator, and negative control was normal saline.
[0048]
Embodiment 3
[0049] Embodiment 3 thrombin (batroxobin) and blood coagulation factor X activator alone and synergistic in vivo hemostasis test
[0050] In accordance with the clinical research guidelines, the in vivo coagulation test adopts the mouse tail docking method. Combined with the clinical dosage of thrombin-like enzyme (batroxobin) we choose: 20~0.1 unit / 60kg This range selects four points respectively for thrombin-like enzyme (batroxobin) and blood coagulation factor X activator as the blood coagulation in mice In the test, the dosage of the mice was adjusted accordingly according to the human dosage, and the dosage adjusted according to the relevant ratio was 8-0.04u / kg.
[0051] The specific method is as follows: get coagulation factor X activator and thrombin-like enzyme (batroxobin) respectively to configure solutions with appropriate concentrations as the test solution.
[0052] Take healthy mice, divide them into 7 groups at random, 5 in each group, inject 0.5ml into the ta...
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