Chemical prepn process of composite hydroxyapatite/collagon layer on the surface of Ni-Ti alloy

Abstract

The present invention belongs to the field of biological bone material preparing technology. The chemical preparation process of composite hydroxyapatite / collagen layer on the surface of Ni-Ti alloy includes the following steps: polishing, washing and drying the Ni-Ti alloy substrate and acid-alkali treatment; pre-calcifying of the substrate inside Na2HOP4 and Ca(OH)2 solution; and finally setting in simulating body fluid containing collagen to grow the composite hydroxyapatite / collagen layer. The process has the features of simple activating treatment and low acid-alkali treatment temperature. In the simulating body fluid containing collagen, hydroxyapatite and collagen are deposited simultaneously to form the composite hydroxyapatite / collagen layer with homogeneous surface and firm combination to the substrate. The present invention is suitable for implant with complicated shape.

Description

Technical field:

[0001] The invention relates to a chemical preparation method of a hydroxyapatite / collagen composite layer on the surface of a nickel-titanium alloy, which belongs to the preparation technology of biological bone materials. Background technique:

[0002] On the basis of analyzing the micro-assembly, biological function and formation mechanism of natural biomaterials, the developed biomedical biomaterials are widely used in the repair and replacement of human tissues and organs. Nickel-titanium memory alloy not only has a special shape memory effect, but also has high strength, small specific gravity, low elastic modulus, and good fatigue strength, which has attracted widespread attention. However, the application of nickel-titanium alloy has been questioned and limited due to its poor biocompatibility. If a coating with better biocompatibility and stability is covered on the surface of NiTi alloy, the good mechanical properties of NiTi alloy and the excelle...

Examples

example 1

[0011] Take a NiTi alloy matrix bulk sample (10×10×3mm 3 ), polished to 800# step by step with water sandpaper, then ultrasonically cleaned in deionized water, and then sealed and dried at room temperature. The dried sample was put into 30% nitric acid and treated at 50°C for 30min. After ultrasonic cleaning with deionized water and airtight drying at room temperature, place it in a sodium hydroxide solution with a concentration of 1.2M and soak for 2 hours at 100°C. After that, the sample needs to be ultrasonically cleaned with deionized water and airtightly dried at room temperature deal with. The obtained sample was placed in Na 2 HPO 4 Soak in a supersaturated aqueous solution at 40°C for 8 hours, and then in Ca(OH) 2 Soak in supersaturated aqueous solution at 25°C for 8h. After that, the pre-calcified sample was ultrasonically cleaned with deionized water and airtightly dried at room temperature, and then soaked in the composition: Na + 213.0mmol / L, Cl - 187.5mmo...

example 2

[0013] Take a NiTi alloy matrix bulk sample (10×10×3mm 3 ), polished to 800# step by step with water sandpaper, then ultrasonically cleaned in deionized water, and then sealed and dried at room temperature. The dried sample was put into 50% nitric acid and treated at 60°C for 10 minutes. After ultrasonic cleaning with deionized water and airtight drying at room temperature, place in 2M sodium hydroxide solution and soak for 5 hours at 100°C. After that, the sample needs to be ultrasonically cleaned with deionized water and airtightly dried at room temperature. . The obtained sample was placed in Na 2 HPO 4 Soak in a supersaturated aqueous solution at 40°C for 4 hours, and then in Ca(OH) 2 Soak in supersaturated aqueous solution at 40°C for 12h. After that, the pre-calcified sample was ultrasonically cleaned with deionized water and airtightly dried at room temperature, and then soaked in the composition: Na + 213.0mmol / L, Cl - 187.5mmol / L, HCO 3 - 40.5mmol / L, K + ...

example 3

[0015] Take a NiTi alloy matrix bulk sample (10×10×3mm 3 ), polished to 800# step by step with water sandpaper, then ultrasonically cleaned in deionized water, and then sealed and dried at room temperature. The dried sample was put into 30% nitric acid and treated at 60°C for 30min. After ultrasonic cleaning with deionized water and airtight drying at room temperature, place it in a sodium hydroxide solution with a concentration of 1.2M and soak for 6 hours at 100°C. After that, the sample needs to be ultrasonically cleaned with deionized water and airtightly dried at room temperature deal with. The obtained sample was placed in Na 2 HPO 4 Soak in a supersaturated aqueous solution at 40°C for 15 hours, and then in Ca(OH) 2 Soak in supersaturated aqueous solution at 25°C for 8h. After that, the pre-calcified sample was ultrasonically cleaned with deionized water and airtightly dried at room temperature, and then soaked in the composition: Na + 213.0mmol / L, Cl - 187.5mm...

Technical field:

[0001] The invention relates to a chemical preparation method of a hydroxyapatite / collagen composite layer on the surface of a nickel-titanium alloy, which belongs to the preparation technology of biological bone materials. Background technique:

[0002] On the basis of analyzing the micro-assembly, biological function and formation mechanism of natural biomaterials, the developed biomedical biomaterials are widely used in the repair and replacement of human tissues and organs. Nickel-titanium memory alloy not only has a special shape memory effect, but also has high strength, small specific gravity, low elastic modulus, and good fatigue strength, which has attracted widespread attention. However, the application of nickel-titanium alloy has been questioned and limited due to its poor biocompatibility. If a coating with better biocompatibility and stability is covered on the surface of NiTi alloy, the good mechanical properties of NiTi alloy and the excelle...

Examples

example 1

[0011] Take a NiTi alloy matrix bulk sample (10×10×3mm 3 ), polished to 800# step by step with water sandpaper, then ultrasonically cleaned in deionized water, and then sealed and dried at room temperature. The dried sample was put into 30% nitric acid and treated at 50°C for 30min. After ultrasonic cleaning with deionized water and airtight drying at room temperature, place it in a sodium hydroxide solution with a concentration of 1.2M and soak for 2 hours at 100°C. After that, the sample needs to be ultrasonically cleaned with deionized water and airtightly dried at room temperature deal with. The obtained sample was placed in Na 2 HPO 4 Soak in a supersaturated aqueous solution at 40°C for 8 hours, and then in Ca(OH) 2 Soak in supersaturated aqueous solution at 25°C for 8h. After that, the pre-calcified sample was ultrasonically cleaned with deionized water and airtightly dried at room temperature, and then soaked in the composition: Na + 213.0mmol / L, Cl - 187.5mmo...

example 2

[0013] Take a NiTi alloy matrix bulk sample (10×10×3mm 3 ), polished to 800# step by step with water sandpaper, then ultrasonically cleaned in deionized water, and then sealed and dried at room temperature. The dried sample was put into 50% nitric acid and treated at 60°C for 10 minutes. After ultrasonic cleaning with deionized water and airtight drying at room temperature, place in 2M sodium hydroxide solution and soak for 5 hours at 100°C. After that, the sample needs to be ultrasonically cleaned with deionized water and airtightly dried at room temperature. . The obtained sample was placed in Na 2 HPO 4 Soak in a supersaturated aqueous solution at 40°C for 4 hours, and then in Ca(OH) 2 Soak in supersaturated aqueous solution at 40°C for 12h. After that, the pre-calcified sample was ultrasonically cleaned with deionized water and airtightly dried at room temperature, and then soaked in the composition: Na + 213.0mmol / L, Cl - 187.5mmol / L, HCO 3 - 40.5mmol / L, K + ...

example 3

[0015] Take a NiTi alloy matrix bulk sample (10×10×3mm 3 ), polished to 800# step by step with water sandpaper, then ultrasonically cleaned in deionized water, and then sealed and dried at room temperature. The dried sample was put into 30% nitric acid and treated at 60°C for 30min. After ultrasonic cleaning with deionized water and airtight drying at room temperature, place it in a sodium hydroxide solution with a concentration of 1.2M and soak for 6 hours at 100°C. After that, the sample needs to be ultrasonically cleaned with deionized water and airtightly dried at room temperature deal with. The obtained sample was placed in Na 2 HPO 4 Soak in a supersaturated aqueous solution at 40°C for 15 hours, and then in Ca(OH) 2 Soak in supersaturated aqueous solution at 25°C for 8h. After that, the pre-calcified sample was ultrasonically cleaned with deionized water and airtightly dried at room temperature, and then soaked in the composition: Na + 213.0mmol / L, Cl - 187.5mm...

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