Facility vegetable mycorrhiza production method
A production method and technology of mycorrhizal seedlings, applied in the field of production of vegetable mycorrhizal seedlings in facilities, can solve the problems of serious soil salinization, poor facility environment regulation ability, soil continuous cropping obstacles, etc., and achieve enhanced disease resistance, salt tolerance and drought tolerance , Improve fertilizer and water utilization rate and fertilizer efficiency, and reduce the effect of soil-borne diseases
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[0030] Embodiment: the production method of facility vegetable mycorrhizal seedling
[0031] (1) Propagation of strains and production of bacterial agents: plant susceptible crops cucumber, corn, sorghum or clover by inoculating strains on sterilized organic soil or soil, and cultivate plants to allow mycorrhizal microorganisms to amplify and reproduce in root growth The fibrous roots and mycorrhizal spores obtained with high density and high degree of infection can be used for the production of mycorrhizal seedlings as inoculants. (The root mycorrhizal infection rate (F) of susceptible crops is more than 70%, the infection intensity (M) is more than 40%, and the relative mycorrhizal rate (a) is more than 20% as the inoculum standard)
[0032] (2) Disinfection of seedling soil: Sterilization is mainly to eliminate various bacteria in the soil. The method is to put the seedling soil or seedling substrate into an oven at 160°C for 2 hours, then continue to dry at 160°C for 2 hou...
experiment example 1
[0035] Experimental Example 1: Comparison of the propagation effects of inoculating G.m mycorrhizal agents on different substrates (Table 1)
[0036] Utilize cultivated cucumber to inoculate AMF in different cultivation substrates, compare its infection rate, obtain the AMF inoculum agent with good infection rate, its infection effect is shown in table (table 1), can be seen from the table and adopt organic soil to proliferate The mycorrhizal rate of the inoculum is significantly higher than that of the original inoculum purchased in Hungary, so the AMF inoculum propagated with organic soil can be directly used in production tests, followed by the inoculum propagated in the seedling substrate prepared by peat and vermiculite.
[0037] Table 1 Comparison of root infection rate of AMF in different seedling substrates
[0038]
experiment example 2
[0039] Experimental Example 2: Comparison of different AMF bacterial strain seedling raising conditions (Table 2):
[0040] Table 2 Effects of different AMF strains on dry matter weight, mycorrhizal infection rate and mycorrhizal dependence of tomato seedlings
[0041]
[0042] The tested strains were Glomus diuphauam (G.d), Glomus mossea (BEG167), Glomus intraradices (BEG141), Glomus etuni-catuml (BEG168), Glomus versiforme (G.v) and Glomus mosseae-2 (G.m).
[0043] Conclusion: From the above experiments, it can be seen that the mycorrhizal infection rate and mycorrhizal dependence of different AMFs on tomato roots are also significantly different. Although the dry weights of shoots and roots of G.m and G.v treatments are not much different, they are similar to The other treatments had extremely significant differences, indicating that they had the strongest effect on promoting tomato growth. It can be seen that different strains have different effects. Glomusmossea-2 (G....
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