Aspergillus niger strain with Agrobacterium tumefaciens inhibiting effect and its use

A technology of Agrobacterium tumefaciens and Aspergillus niger strains, applied in the fields of application, fungi, and methods based on microorganisms, can solve problems such as storage and ready-to-use, affecting the production of fruit tree products, and affecting the development of agricultural economy, etc., to achieve Reasonable technical route, easy cultivation and preservation, and strong tolerance

Inactive Publication Date: 2006-10-18
GUIZHOU UNIV
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Problems solved by technology

[0003] Root cancer is a worldwide disease that does great harm to fruit trees, directly affecting the production of fruit tree products, thus affecting the development of agricultural economy
Therefore, the prevention and treatment of this disease has attracted the attention of countries all over the world. In view of the pollution of the environment and the destruction of ecological balance by chemical pesticides, the biological control of plant root cancer has been paid attention to. Bacteriocins are used to prevent and inhibit the growth of Agrobacterium tumefaciens, but the strains are mainly non-pathogeni

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0021] Isolation steps of Aspergillus niger xj that has inhibitory effect on Agrobacterium tumefaciens:

[0022] (1) The tested strain: Agrobacterium tumefaciens T-37 was purchased from the Soil and Fertilizer Research Institute of the Chinese Academy of Agricultural Sciences and kept in the Institute of Fungal Resources of Guizhou University;

[0023] (2) Separation and purification of antagonistic bacteria: accurately weigh 10.0g of fruit tree root soil sample, with the help of aseptic technique, add the soil sample into a triangular flask containing 90ml of sterile water and glass beads, shake for 10 minutes to make the soil Disperse the sample evenly into a diluent, and then absorb 1m of the soil diluent into 9ml of sterile water, mix well, and become a soil suspension. Inoculate by mixed bacteria method, draw 1ml of suspension into a sterile petri dish with a diameter of 9cm, then pour the double-anti-Martin's medium that has been melted and cooled to 45°C, mix well, and afte...

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Abstract

The present invention discloses one kind of Aspergillus niger strain possessing Agrobacterium tumefaciens inhibiting effect. Morphologically, it features that after Aspergillus niger strain is cultured in Czapek's medium at 26 deg.c for 10 days, colony of diameter 4.0 cm and with black carbon colored surface is formed and there are compactly inserted spore head, overspread conidiophore structures, developed aerial hyphae, and aerial hyphae differentiated conidiophores. The present invention has wide applicable natural condition range, good tolerance on poor environment, easy culturing, preservation and industrial production, and high Agrobacterium tumefaciens inhibiting effect.

Description

technical field [0001] The invention relates to an Aspergillus niger strain capable of inhibiting Agrobacterium tumefaciens and its application. Background technique [0002] Root cancer caused by Agrobacterium tumefaciens is distributed all over the world. DeCleene and DeLey have reported that Agrobacterium tumefaciens can infect dicotyledonous plants and a few gymnosperms in 93 families, 331 genera and 643 genera. In 1980, Kennedy reported that among the crop losses caused by prokaryotic plant pathogens in the United States, root cancer of fruit trees and grapes ranked as the most important. Grape root cancer occurs in varying degrees in 13 provinces in the northern region of my country. Cherry root cancer occurs seriously in Shandong, Dalian, Hebei and other places. Peach tree root cancer occurs commonly in Jiangsu, Zhejiang, Fujian and the suburbs of Shanghai. Severe orchard plant disease rate is more than 90%. [0003] Root cancer is very harmful to fruit trees and is...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04C12R1/685
Inventor 李祝梁宗琦肖洋周礼红李颖
Owner GUIZHOU UNIV
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