Aspergillus niger strain with Agrobacterium tumefaciens inhibiting effect and its use

Abstract

The present invention discloses one kind of Aspergillus niger strain possessing Agrobacterium tumefaciens inhibiting effect. Morphologically, it features that after Aspergillus niger strain is cultured in Czapek's medium at 26 deg.c for 10 days, colony of diameter 4.0 cm and with black carbon colored surface is formed and there are compactly inserted spore head, overspread conidiophore structures, developed aerial hyphae, and aerial hyphae differentiated conidiophores. The present invention has wide applicable natural condition range, good tolerance on poor environment, easy culturing, preservation and industrial production, and high Agrobacterium tumefaciens inhibiting effect.

Description

technical field [0001] The invention relates to an Aspergillus niger strain capable of inhibiting Agrobacterium tumefaciens and its application. Background technique [0002] Root cancer caused by Agrobacterium tumefaciens is distributed all over the world. DeCleene and DeLey have reported that Agrobacterium tumefaciens can infect dicotyledonous plants and a few gymnosperms in 93 families, 331 genera and 643 genera. In 1980, Kennedy reported that among the crop losses caused by prokaryotic plant pathogens in the United States, root cancer of fruit trees and grapes ranked as the most important. Grape root cancer occurs in varying degrees in 13 provinces in the northern region of my country. Cherry root cancer occurs seriously in Shandong, Dalian, Hebei and other places. Peach tree root cancer occurs commonly in Jiangsu, Zhejiang, Fujian and the suburbs of Shanghai. Severe orchard plant disease rate is more than 90%. [0003] Root cancer is very harmful to fruit trees and is...

AI Technical Summary

Problems solved by technology

[0003] Root cancer is a worldwide disease that does great harm to fruit trees, directly affecting the production of fruit tree products, thus affecting the development of agricultural economy

Therefore, the prevention and treatment of this disease has attracted the attention of countries all over the world. In view of the pollution of the environment and the destruction of ecological balance by chemical pesticides, the biological control of plant root cancer has been paid attention to. Bacteriocins are used to prevent and inhibit the growth of Agrobacterium tumefaciens, but the strains are mainly non-pathogenic Agrobacterium rhizogenes K84 strains. The role of K84 strains in the prevention and treatment of root cancer is to prevent non-cure. It is used as a preventive measure and does not prevent all root cancers, it is only effective against Agrobacterium tumefaciens containing the nopaline Ti plasmid

Although some other non-pathogenic Agrobacteria isolated, such as Agrobacterium tumefaciens J73 and Agrobacterium tumefaciens D286, can make up for the deficiency of the K84 strain, the biocontrol bacteria are not drought-tolerant and have a short lifespan, so they must be stored and processed with them. Ready to use