Biodegradable material microsphere blood vessel suppository containing liposome cell factor and its preparation and uses

A technology of vascular embolic agents and cytokines, which is applied in the field of microsphere vascular embolic agents containing biodegradable materials containing liposome cytokines and its preparation and application, can solve problems such as poor stability, limited clinical application, and short half-life. Achieve strong hydration, improve curative effect, and reduce toxic and side effects

Active Publication Date: 2006-12-13
BEIJING SHENGYIYAO SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Due to the use of cytokines and their application in the body can produce many adverse reactions, limiting its clinical application
IL-2 has a short half-life in the b

Method used

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  • Biodegradable material microsphere blood vessel suppository containing liposome cell factor and its preparation and uses
  • Biodegradable material microsphere blood vessel suppository containing liposome cell factor and its preparation and uses
  • Biodegradable material microsphere blood vessel suppository containing liposome cell factor and its preparation and uses

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Comparison scheme
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Embodiment Construction

[0055] Preparation of IL-2 Liposomal Cytokine

[0056] 1. Materials and methods (reverse-phase evaporation method)

[0057] Membrane material: soybean lecithin (SYPC): cholesterol=1:1 (molar ratio) The organic phase was prepared with ether.

[0058] Water phase: IL-2 0.1M PBS. Organic phase: water phase = 3:1 Dissolve the membrane material in 30 ml of ether, add IL-2 solution, sonicate for 2-3 minutes between ice baths (0.5 minutes, 0.5 minutes) to form a homogeneous emulsion, and the ether was removed by rotary evaporation at 20-25 degrees to obtain a white homogeneous liposome suspension.

[0059] 2. Encapsulation rate determination

[0060] The prepared liposome suspension was centrifuged at high speed and low temperature (30000G, 60 minutes at 4 degrees), the supernatant was absorbed, the precipitate was washed with PBS, and centrifuged again, and repeated twice, and the centrifuged lower liposomes were added to ether to make the water phase. Separate from the lipid pha...

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Abstract

The invention discloses the degradable biomaterial microsphere vessel suppository and preparing method. The method comprises the following steps: extracting polysaccharose sodium salt or gelatin from natural agonic acid, using immunocyte factor, such as interleukins-2, tumor necrosis factor and interferon, as presidium, making liposome cytokine micro spheres whose sizes are 50 mum-75 mum, 75 mum-150 mum, 100 mum-200 mum, 200 mum-300 mum, 200 mum-450 mum, 100 mum-300 mum, 300 mum-500 mum, 500 mum-700 mum, and 700 mum-900 mum. The degradable biomaterial microsphere vessel suppository can be used to cure late hepatic carcinoma, kidney cancer, bladder cancer, carcinoma of colon, and other carcinoma arterial embolisms.

Description

technical field [0001] The present invention relates to a vascular embolic agent of biodegradable materials and its preparation and application, in particular to polysaccharide sodium salt or gelatin coated with lipid which is extracted from natural plant alginic acid and has good biodegradability and biocompatibility. Cytokines wrapped in plastids, made into microspheres of 50μm-75μm, 75μm-150μm, 100μm-200μm, 200μm-300μm, 200μm-450μm, 100μm-300μm, 300μm-500μm, 500μm-700μm, 700μm-900μm microspheres, used for Tumor arterial embolization and locally targeted immunochemotherapy. Background technique [0002] Tumor arterial embolization therapy is a new therapy that injects embolic substances into the arterioles dominating the cancer tissue to cause mechanical embolization of the arterioles, thereby inhibiting tumor growth. In 1981, Kato first proposed chemoembolization, which combined chemotherapy drugs and embolic substances effectively and organically, and proposed a new met...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61K38/20A61K38/21A61K9/16A61K9/14A61K9/127A61K47/36A61P35/00
Inventor 李小平崔恒魏丽惠冯捷洪宏李新建齐宪荣
Owner BEIJING SHENGYIYAO SCI & TECH DEV
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