Organism preparation method of agricultural amino acid and fertilizer product
An amino acid and product technology, applied in the directions of organic fertilizer, organic fertilizer preparation, biological organic part treatment, etc., can solve problems such as threats to tobacco production and sustainable development of tobacco leaves, sharp reduction of soil microbial activity, and poor control of decomposing degree. Achieve the effect of low product price, stimulating plant growth and less investment in fixed assets
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[0017] 1. Expanded culture of liquid strains
[0018] Preserved strains → slant activation (NB medium, cultured at 37 degrees for 24-48 hours) → shake flask seed culture (seed medium, cultured at 37 degrees for 24-36 hours, speed 120-200 rpm) → fermented seed culture ( Seed medium, 37 degrees seed tank culture for 24-36 hours) → fermentation culture (liquid fermentation production formula, 37 degrees fermentation tank culture for 24-36 hours) → fermentation liquid → solid matter adsorption to form solid fermentation strains → low temperature (4- 10 degrees) save for later use.
[0019] The formula of the seed culture medium is: 0.1-0.5% of beef extract, 0.25-0.8% of peptone, 0.15-0.7% of glucose, 0.15-0.6% of sodium chloride, and pH7.0-7.5.
[0020] The formula of the liquid fermentation production medium is: 1.25-2.0% of corn flour, 1.8-3.0% of cake, 0.2-0.75% of sodium chloride, 0.01-0.1% of sodium carbonate, and 0.1%-0.2% of amylase.
[0021] 2. Preparation of solid ferme...
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