Organism preparation method of agricultural amino acid and fertilizer product

[0017] 1. Expanded culture of liquid strains

[0018] Preserved strains → slant activation (NB medium, cultured at 37 degrees for 24-48 hours) → shake flask seed culture (seed medium, cultured at 37 degrees for 24-36 hours, speed 120-200 rpm) → fermented seed culture ( Seed medium, 37 degrees seed tank culture for 24-36 hours) → fermentation culture (liquid fermentation production formula, 37 degrees fermentation tank culture for 24-36 hours) → fermentation liquid → solid matter adsorption to form solid fermentation strains → low temperature (4- 10 degrees) save for later use.

[0019] The formula of the seed culture medium is: 0.1-0.5% of beef extract, 0.25-0.8% of peptone, 0.15-0.7% of glucose, 0.15-0.6% of sodium chloride, and pH7.0-7.5.

[0020] The formula of the liquid fermentation production medium is: 1.25-2.0% of corn flour, 1.8-3.0% of cake, 0.2-0.75% of sodium chloride, 0.01-0.1% of sodium carbonate, and 0.1%-0.2% of amylase.

[0021] 2. Preparation of solid fermentation material

[0022] Dry mix the raw material cake with 1-2% calcium oxide and sodium bicarbonate for 5-10 minutes, then add about 1m of tap water while stirring 3 Around, the water content is controlled at 60-65%, the pH is adjusted to 6.0-7.5, and the materials are required to be mixed evenly, loose and not agglomerated.

[0023] 3. Solid fermentation regulation and control

[0024]In the prepared material, inoculate the liquid strain (fermentation liquid) according to the inoculum amount of 1-5%, build a heap (about 2m in height) of the inoculated material to ferment, observe the temperature and pH changes of the heap, and start when the heap temperature rises to 50 degrees Turn over the pile for the first time, and the temperature of the pile will continue to rise rapidly in the next 1-2 days. Pay attention to turning over the pile to cool down, and keep the pile temperature below 50 degrees. During the fermentation process, the pH of the material drops first and then rises. When the pH rises to 8.0, ammonia At this time, combined with the turning process, the MSG waste liquid (pH1-3) is sprayed to adjust the pH and water content, so that the pH is always maintained at 6.0-7.5, and the water content of the material is maintained at 60-65%. Continue to ferment, after 5 days There is a certain ammonia smell to stop the enzymatic hydrolysis process, immediately spray the waste liquid of monosodium glutamate to reduce the pH to about 5.0, and at the same time carry out low-temperature drying or air drying to obtain a mixed material containing 10%-12% of free amino acids.

[0025] 4. Manufacture of amino acid-containing fertilizer products

[0026] The obtained amino acid mixed material after enzymatic hydrolysis can be directly used as fertilizer, and can also be used as raw material for producing amino acid organic fertilizer, amino acid organic-inorganic compound fertilizer and amino acid foliar fertilizer. Field biological test results also show that direct application of this amino acid mixture fertilizer increases the yield by 16% (compared with fertilizers of equal price) than direct application of raw material cakes; Fertilizer yield increases by 9% (compared with equal-priced fertilizers); using the amino acid foliage fertilizer produced from the amino acid mixture as a raw material increases yield by 6% (while the product price is 50% lower) than the amino acid foliage fertilizer obtained by chemical hydrolysis.

[0027] With the amino acid-containing mixed material obtained by the inventive method as the main raw material after crushing, compound with organic compost to make amino acid-containing organic fertilizer (see table 1), and compound with inorganic fertilizer to make amino acid-containing organic-inorganic compound fertilizer (see table 2 ), granulated and dried to make corresponding products.

[0028] total organic matter

[0029] total organic matter

[0030] The developed amino acid-containing organic fertilizer (Table 1) has a very significant effect on increasing the yield of tomato and watermelon crops on the continuous cropping soil after being applied to the soil (see Table 3). Applying this product, the yield of tomato and watermelon is very low, while the yield on the same soil without continuous cropping obstacles is higher, indicating that the soil used in the test has serious continuous cropping obstacles. After applying this product on the soil with continuous cropping obstacles, the yield of tomato and watermelon Yield increased (the 3rd line of Table 3), and they respectively reached 84.8% and 85.2% of the soil yield without continuous cropping obstacles, indicating that this product is very obvious in overcoming the effect of economic crops continuous cropping obstacles.

[0031] Fresh weight of tomato fruit (g/plant)

[0032] Note: All fertilizers are applied at one time

[0033] The developed amino acid-containing organic-inorganic compound fertilizer (table 2) is very obvious to the promotion of tobacco growth and tobacco leaves. From table 4, it can be seen that the tobacco plant height, The leaf number and leaf size are significantly higher than those treated with local organic-inorganic compound fertilizer and local conventional fertilization. It can also be seen from the field growth and appearance that the tobacco treated with amino acid organic-inorganic compound fertilizer is better than the other two treatments.

[0034] Table 4: Effects of amino acid-containing organic-inorganic compound fertilizers on tobacco growth

[0035]

[0036] Note: T1: amino acid organic-inorganic compound fertilizer, T2: local organic-inorganic compound fertilizer, T3: local conventional fertilization.

[0037] It can be seen from Table 5 that the tobacco yield and the proportion of top-grade tobacco leaves treated with amino acid organic-inorganic compound fertilizers are significantly higher than those of local organic-inorganic compound fertilizers and local conventional fertilization. Therefore, the application of the amino acid organic-inorganic compound fertilizer can significantly increase the economic income of tobacco farmers, showing that the fertilizer has a broad market prospect.

[0038] deal with

[0039] Note: T1: amino acid organic-inorganic compound fertilizer, T2: local organic-inorganic compound fertilizer, T3: local conventional fertilization.