Toad skin extract and its medicine prepn and their prepn

A technology of extracts and traditional Chinese medicine preparations, applied in the field of toad skin extracts, can solve the problems of incomplete extraction of active ingredients, high impurity content, high ointment rate, etc., and achieve the effect of convenient clinical use, small dosage, and small volume

Inactive Publication Date: 2007-03-21
周亚球
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

"A preparation method of cinobufacin freeze-dried powder injection" (patent application number 200510115451.0), in which the toad skin extract is still extracted by water and alcohol precipitation method, the extraction of active ingredients is not complete, the cream yield is high, and the impurity content is large

Method used

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Examples

Experimental program
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preparation example Construction

[0035] The preparation method of the present embodiment toad skin extract is to operate as follows:

[0036] 1. Take dry toad skin, wash it, add 6-7 times the amount of 85% methanol or ethanol or acetone to soak for 2 hours, reflux extraction for 60 minutes, let it cool, filter, add 5-6 times the amount of 85% methanol or ethanol or Acetone, reflux extraction for 50 minutes, let cool, filter, and combine the filtrates;

[0037] 2. The filtrate obtained in step 1 is concentrated under reduced pressure until it has no solvent smell, and is dissolved by adding water equivalent to 1 to 2 times the amount of medicinal materials, and filtered after standing for 24 hours;

[0038] 3. The macroporous adsorption resin column on the filtrate obtained in step 2, adopts medium polarity or polar macroporous adsorption resin, specifically polystyrene type or cross-linked propylene resin, including HPD-400, DM301 or HPD-600, The particle size is 0.3mm~1.25mm. First use 2 to 3 times of colu...

Embodiment 1

[0040] Take 500g of dried toad skin, wash it, soak in 6 times the amount of 85% methanol for 2 hours, reflux for extraction for 60 minutes, let cool, filter, add 5 times the amount of 85% methanol to the residue, reflux for 50 minutes, let it cool and filter, and combine the filtrates.

[0041] Concentrate the filtrate under reduced pressure until it has no alcohol smell, add 1.5 times the amount of water to dissolve it, let it stand for 24 hours, and filter.

[0042] The filtrate was put on a HPD-400 type macroporous adsorption resin column, first eluted with 3 times column volume of water, and then 4 times column volume of 50% ethanol, collected the ethanol eluate, concentrated under reduced pressure, and dried in vacuo at 60°C. Get toad skin extract 4.0g.

Embodiment 2

[0044] Take 500g of dry toad skin, wash it, add 6 times the amount of 85% ethanol to soak for 2 hours, reflux extraction for 60 minutes, let cool, filter, add 5 times the amount of 85% ethanol to the residue, reflux extraction for 50 minutes, let it cool, filter, and combine the filtrates .

[0045] Concentrate the filtrate under reduced pressure until there is no alcohol smell, add 2 times the amount of water equivalent to the medicinal materials, dissolve, let stand for 24 hours, and filter.

[0046] The filtrate was put on a HPD-600 type macroporous adsorption resin column, first eluted with 3 times column volume of water, and then 3 times column volume of 50% ethanol, collected the ethanol eluate, concentrated under reduced pressure, and dried in vacuo at 60°C. Get toad skin extract 3.5g.

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Abstract

The present invention is toad skin extract and its medicine preparation and their preparation, and features that the toad skin extract has indole alkaloid content of 25-40 % and total cinobufagin and lipobufogenin content of 0.01-0.05 %. During the preparation, the indole alkaloid content and the total cinobufagin and lipobufogenin content is raised as possible. The said Chinese medicine preparation has high quality, small size, small dosage, high clinical curative effect and lowered side effect.

Description

Technical field: [0001] The invention relates to a toad skin extract, a preparation method of the extract and various traditional Chinese medicine preparations prepared by adding the toad skin extract to medicinal auxiliary materials. Background technique: [0002] The toad skin is the dried whole skin of Bufo bufo gargarizans Cantor or Bufomelanostictus Schneider, which are Bufoidae animals. The chemical components of toad skin mainly include cinobufonitoxin, repobufagenin, bufalin, etc., and the esters formed by them with succinic acid, succinic acid, arginine, etc., such as bufafolin-3- Succinyl arginine ester, bufofabin-3-succinyl arginate, cinobufagin-3-succinyl arginine ester, cinobufagenin-3-succinyl arginate Amino acid ester etc. It also contains indole alkaloids, such as 5-hydroxytryptamine, bufatryptamine, bufaquatamine, bufotinin and dehydrobufatryptamine, etc., and also contains amino acids, polypeptides and sterol compounds. Toad skin has the functions of cle...

Claims

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Application Information

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IPC IPC(8): A61K35/56A61P35/00A61P1/16A61P31/20A61P17/06A61P17/12A61P43/00A61K35/65
Inventor 周亚球刘金旗吴涛
Owner 周亚球
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