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Process of extracting effective component from papaya

An active ingredient, the technology of papaya, applied in the field of extracting active ingredients of papaya, can solve the problem of easy oxidation in the purification process, and achieve high selectivity

Inactive Publication Date: 2007-03-21
王有才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a method for extracting active ingredients from papaya, so as to overcome the defect that the purification process of the prior art is easy to oxidize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Detection method:

[0017] Determination of flavonoid content:

[0018] Flavonoid extraction: Weigh about 0.5g of dry sample, add 10ml of 70% ethanol to soak, shake intermittently for 6 hours at 70°C, filter with G3 sand core funnel, add 10ml of 70% ethanol to soak, and soak at 70°C Intermittent shaking for 6h, repeated 3 times. Combine the filtrate, dilute to 50ml, to be tested;

[0019] Determination of flavonoids: make standard curve: take 18 50ml volumetric flasks, draw 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34ml 100ug / L rutin solution (prepared in 30% ethanol), add 10ml 30% ethanol, 0.7ml 5% NaNO2, add 10% Al(NaNO3)3 after 5min, add 5ml 40%NaOH after 6min, mix well, use Dilute to volume with 30% ethanol, develop color in a water bath at 35°C, and measure OD510nm at 510nm after 10 minutes to obtain the regression equation: Y(100ug / ml)=0.5605+91.9819 OD510nm[r=0.99 ** ], and then convert to calculate the content of flavonoids.

[0020] De...

Embodiment 2

[0029] The dry powder of 1 kg of wrinkled papaya was placed in the extraction kettle, and CO was continuously fed at a speed of 2 kg / hour. 2 Carry out extraction and elution, collect the CO that the extraction time is 0.5 hours flowing out of the extraction kettle 2 , the pressure is relieved to normal pressure, and the residue after pressure relief is obtained, which is the active ingredient of wrinkled papaya.

[0030] CO in the extraction kettle 2 The temperature is 32℃, and the pressure is 8~15Mpa.

[0031] Adopt the method of embodiment 1 to detect, wherein:

[0032] Total flavonoids: 460.25mg / 100g FW, Vc: 209.35mg / 100g FW, total saponins: 896.44mg / 100g FW, oleanolic acid: 62.15mg / 100g FW.

[0033] Example 2

[0034] The dry powder of 1 kg of wrinkled papaya pulverized was placed in the extraction kettle, and CO was continuously fed at a speed of 25 kg / hour. 2 Carry out extraction and elution, add 10 kilograms of 35% aqueous ethanol solutions ...

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PUM

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Abstract

The process of extracting effect component from papaya includes the following steps: setting crushed dry papaya powder inside an extracting kettle, introducing supercritical CO2 to extract and elute, collecting CO2 effluent in the extracting time of 0.5-5 hr, relieving pressure to normal pressure, blowing down CO2 or pressurizing for reuse, and collecting the remainder after pressure relief as the required effective component. The said process of extracting effective component in papaya has high selectivity and the product can meet relevant requirement.

Description

technical field [0001] The invention relates to a method for extracting effective components of papaya. Background technique [0002] Chaenomeles speciosa (Sweet) Nakai [C.lagenaria (Loisel) Koidz], is the fruit of the Rosaceae papaya plant. [0003] According to modern medical evidence, the wrinkled papaya fruit contains: saponins, malic acid, vitamins, superoxide dismutase (referred to as SOD) and flavonoids and other nutrients needed by the human body. It mainly has the functions of relaxing meridians and activating collaterals, and eliminating dampness in the stomach, enhancing collective immune function; reducing liver cell necrosis, promoting its repair, preventing liver cell swelling and balloon deformation, and significantly reducing serum alanine aminotransferase levels; Tumor effect; has obvious bacteriostatic effect on Staphylococcus, in addition to Shigella Shigella, Shigella flexneri, Shigella sonnei and its variants, pathogenic Escherichia coli, common Escheri...

Claims

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Application Information

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IPC IPC(8): A61K36/732A61P37/04A61P1/14A61P1/16A61P31/04A61P35/00A61K131/00
Inventor 王有才
Owner 王有才