Controlled release formulation for anti entity tumour
A kind of technology of sustained-release preparation and sustained-release injection
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Embodiment 1
[0142] Put 80 mg of polylactic acid (PLGA, 50:50) with a peak molecular weight of 25,000-40,000 into a container, add 100 ml of dichloromethane, dissolve and mix well, add 10 mg of collagenase and 10 mg of methotrexate, and re-shake Dry in vacuo to remove the organic solvent. The dried solid composition is shaped immediately, subpackaged and then sterilized by radiation to obtain the anti-cancer sustained release containing 10% collagenase and 10% methotrexate. All are percentages by weight. The drug release time of the anti-solid tumor pharmaceutical composition in physiological saline in vitro is 15-25 days, and the drug release time in mouse subcutaneous is 25-50 days.
Embodiment 2
[0144] As described in Example 1, the difference is that the peak molecular weight of polylactic acid is 35000-60000 (PLGA, 75:25), and the anticancer active ingredient and weight percentage are one of the following:
[0145] (1) 5% hyaluronidase and 10% epothilone, epothilone B, doxorubicin, epirubicin, mitomycin C, actinomycin D or dactinomycin combination; or
[0146] (2) 10% relaxin and 10% fluorouracil, 6-mercaptopurine, methotrexate, flumethorexate, calcium leucovorin, calcium folinate, carmofur, tegafur, and Youfudine , topotecan, a combination of topotecan hydrochloride, cytarabine, cyclocytidine, or hydroxyurea.
Embodiment 3
[0148] Put 80mg of polyphenylpropane (p-carboxyphenylpropane (p-CPP): sebacic acid (SA) at 20:80) copolymer into a container, add 100ml of dichloromethane, dissolve and mix well, then add 10mg of pancreatic Protease and 10 mg fluorouracil were re-shaken and spray-dried to prepare microspheres for injection containing 10% trypsin and 10% fluorouracil. Then suspend the microspheres in physiological saline containing 15% mannitol to prepare the corresponding suspension-type sustained-release injection with a viscosity of 20cp-300cp (at 20°C-30°C). The drug release time of the slow-release injection in physiological saline in vitro is 15-25 days, and the drug release time in mice subcutaneous is about 30-40 days.
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Abstract
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